Telocytes in trachea and lungs

Zheng, Yonghua; Li, Hangchao; Manole, Catalin G.; Sun, Andy; Ge, Jingping; Wang, X.

doi:10.1111/j.1582-4934.2011.01404.x

Cited by 112 publications

(109 citation statements)

References 40 publications

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“…In late gestational stages, CD34 expression is observed in CD31-negative, fibroblast-like cells, with long prolongations. These CD34 ϩ cells have been called "telocytes," and they had been described in the trachea and lungs (16,34,44). However, different from what has been described for telocytes, we could not find c-Kit expression in these CD34 ϩ cells.…”

Section: Discussioncontrasting

confidence: 52%

Wt1-expressing progenitors contribute to multiple tissues in the developing lung

Cano

Carmona

Muñoz‐Chápuli

2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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Cano E, Carmona R, Muñoz-Chápuli R. Wt1-expressing progenitors contribute to multiple tissues in the developing lung. Am J Physiol Lung Cell Mol Physiol 305: L322-L332, 2013. First published June 28, 2013 doi:10.1152 doi:10. /ajplung.00424.2012velop from paired endodermal outgrowths surrounded by a mesodermal mesenchyme. Part of this mesenchyme arises from epithelialmesenchymal transition of the mesothelium that lines the pulmonary buds. Previous studies have shown that this mesothelium-derived mesenchyme contributes to the smooth muscle of the pulmonary vessels, but its significance for lung morphogenesis and its developmental fate are still little known. We have studied this issue using the transgenic mouse model mWt1/IRES/GFP-Cre (Wt1 cre ) crossed with the Rosa26R-EYFP reporter mouse. In the developing lungs, Wt1, the Wilms' tumor suppressor gene, is specifically expressed in the embryonic mesothelium. In the embryos obtained from the crossbreeding, the Wt1-expressing cell lineage produces the yellow fluorescent protein (YFP), allowing for colocalization with differentiation markers. Wt1 cre -YFP cells were very abundant from the origin of the lung buds to postnatal stages, contributing significantly to pulmonary endothelial and smooth muscle cells, bronchial musculature, tracheal and bronchial cartilage, as well as CD34 ϩ fibroblast-like interstitial cells. Thus Wt1 cre -YFP mesenchymal cells show the very same differentiation potential as the splanchnopleural mesenchyme surrounding the lung buds. FSP1 ϩ fibroblast-like cells were always YFP Ϫ ; they expressed the common leukocyte antigen CD45 and were apparently recruited from circulating progenitors. We have also found defects in pulmonary development in Wt1 Ϫ/Ϫ embryos, which showed abnormally fused lung lobes, round-shaped and reduced pleural cavities, and diaphragmatic hernia. Our results suggest a novel role for the embryonic mesothelium-derived cells in lung morphogenesis and involve the Wilms' tumor suppressor gene in the development of this organ. pulmonary development; coelomic epithelium; mesothelium; Wilms' tumor suppressor gene THE DEVELOPMENT OF THE RESPIRATORY system was a key evolutionary event for the air-breathing vertebrates. Lungs arose as specialized endodermal sacs from the digestive tract, and this phylogenetic origin is recapitulated in lung ontogeny. The contribution of mesodermal cells to the endodermal structures is essential for pulmonary development and function. In mouse embryos, the lung primordium sprouts from the ventral foregut by embryonic day (E) 9.5. It consists of a simple tube of endodermal epithelium surrounded by mesenchyme derived from the splanchnic mesoderm and lined by a layer of mesothelium. This primary tube branches in the pair of pulmonary sacs (reviewed in Ref. 17).

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Section: Discussioncontrasting

confidence: 52%

Wt1-expressing progenitors contribute to multiple tissues in the developing lung

Cano

Carmona

Muñoz‐Chápuli

2013

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…TCs have been identified in the interstitial space of many organs, including heart (Popescu et al, 2006;Mandache et al, 2007;Kostin and Popescu, 2009;, pericardium Suciu et al, 2009), pulmonary veins , intestine (Carmona et al, 2011), mesentery , gall bladder (Hinescu et al, 2007), uterus and fallopian tube (Popescu et al, 2005bCretoiu et al, 2009), trachea (Zheng et al, 2011), lungs (Zheng et al, 2011;Popescu et al, 2011a), pleura (Hinescu et al, 2011), skeletal muscle (Popescu et al, 2011b), exocrine pancreas (Popescu et al, 2005c), mammary gland (Gherghiceanu and Popescu, 2005;Popescu et al, 2005a;Radu et al, 2005), or placenta (Suciu et al, 2007(Suciu et al, , 2010. Recently, we firstly identified TCs in mouse trachea and successfully isolated and cultured TCs from mouse lung tissues (Zheng et al, 2011).…”

mentioning

confidence: 98%

Telocyte morphologies and potential roles in diseases

Zheng

Bai

Wang

2012

Journal Cellular Physiology

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Telocytes (TCs) are a new type of interstitial cells, a small cellular body with the presence of 2-5 prolongations named as telopode (Tp)-very thin (less than 0.2 µm) and extremely long (10-1,000 µm), a moniliform aspect, and caveolae, containing a nucleus surrounded by a small amount of cytoplasm. The nucleus occupies about 25% of TC body volume and contains clusters of heterochromatin attached to the nuclear envelope. The perinuclear cytoplasm is rich in mitochondria and contains a small Golgi complex, rough and smooth endoplasmic reticulum and cytoskeletal elements. TCs have several immunophenotypes such as CD34, c-kit, and vimentin. TCs were found in many organs of mammals with potential biological functions, even though the exact function remains unclear. Recently, we identified and isolated TCs from the trachea for the first time and confirmed the existence of TC in lung tissues, which could have the potential significance in the pathogenesis of pulmonary diseases. Future efforts are required to clarify pathophysiological functions of TCs in the disease.

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“…FIB-SEM tomography, the most advanced and powerful technique to visualize cells confirmed the existence of TCs [16]. Since their identification in 2010, TCs have been found in various mammalian organs and tissues and contribute to form a complex interstitial network for the maintenance of tissue homeostasis, such as heart [17][18][19][20][21], lung [22][23][24], placenta [25], pancreas [26,27], skin [28,29], skeletal muscle [30][31][32], uterus [33][34][35][36][37][38], urinary system [39][40][41], and others [42][43][44][45][46][47][48][49][50][51][52][53][54][55]. Furthermore, increasing evidence has demonstrated the presence of TCs within stem cell niches [23,29,31,49,56,57] and indicated the potential involvement of TCs in tissue regeneration/r...…”

Section: Introductionmentioning

confidence: 99%

Telocytes in Pregnancy-Induced Physiological Liver Growth

Wang

Bei

Zhao

et al. 2015

Cell Physiol Biochem

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Background/Aims: We previously documented the presence of Telocytes (TCs) in liver and further indicated the potential roles of TCs in liver regeneration after hepatectomy. Pregnancy-induced liver growth, other than liver regeneration after hepatectomy, is a physiological hepatic adaption to meet the enhanced nutritional and metabolic demands. However, the possible roles of TCs in pregnancy-induced liver growth remain unknown. Methods: Pregnant mice were sacrificed at different time points (pregnancy day 0.5, 4.5, 8.5, 10.5, 12.5, 14.5, 16.5, and 18.5). The liver weight was used to evaluate the liver growth during pregnancy. Hepatocytes proliferation was determined by albumin and 5-ethynyl-2'- deoxyuridine (EdU) double immunostaining while TCs were counted by double immunolabeling for CD34/PDGFR-α. Results: Pregnancy-induced liver growth was preceded by increased proliferation of hepatocytes at pregnancy day 4.5, 8.5, 14.5 and 16.5. Furthermore, the number of TCs in liver detected by double immunolabeling for CD34/PDGFR-α was significantly increased at pregnancy day 4.5 and day 14.5, that was coincident with the occurrence of two peaks of hepatic cell proliferation during pregnancy. Conclusion: Our results suggest a possible relationship between TCs and hepatocyte proliferation in pregnancy-induced liver growth.

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Telocytes in trachea and lungs

Cited by 112 publications

References 40 publications

Wt1-expressing progenitors contribute to multiple tissues in the developing lung

Wt1-expressing progenitors contribute to multiple tissues in the developing lung

Telocyte morphologies and potential roles in diseases

Telocytes in Pregnancy-Induced Physiological Liver Growth

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