Telocytes promote hepatocellular carcinoma by activating the ERK signaling pathway and miR-942-3p/MMP9 axis

Xu, Ying; Tian, Haijun; Luan, Chao; Sun, Kai; Peng, Baojin; Zhang, Hua Yu; Zhang, Nan

doi:10.1038/s41420-021-00592-z

Cited by 14 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study, we elucidated that TCs promoted the metastasis of HCC by secreting MMP9, which is classified as the gelatinase subtype of the MMP family. We also found that multiple growth factors from cancer cells contributed to activating the MAPK signaling pathway, resulting in MMP9 overexpression (Kochi et al, 2020; Xu et al, 2021). Notwithstanding, small molecules take part in the process of oncogenesis, and somatic mutations as a pathogenic element for the onset of malignancy and should not be ignored.…”

Section: Discussionmentioning

confidence: 92%

“…Focusing on tumorigenesis, TCs were reported as an accelerative factor to promote the invasion and migration of cancer and cell proliferation (Klein et al, 2020). For instance, we demonstrated that when PDGF‐α stimulated TCs, the Raf/MEK/ERK signaling pathway was activated to increase MMP9 expression, which was a molecular mechanism by which TCs promote HCC metastasis (Xu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Luan¹,

2022

Cell Biology International

Self Cite

View full text Add to dashboard Cite

Telocytes (TCs) have crucial functions to promote the metastasis of hepatocellular carcinoma (HCC) by over-expressing matrix metalloproteinases (MMPs), but the mechanism by which TCs secrete MMPs in the genome is still unknown. We first cultured and isolated primary TCs from distinct liver cancer tissues and hepatic hemangioma surrounding tissues (Control group). Their whole exon genes were tested by Illumina HiSeq family of platforms and by high-throughput sequencing as well as variant mutations. Moreover, immunohistochemistry, Western blot, and quantitative reverse transcription-polymerase chain reaction assays were utilized to assess the expression of MMPs. The perniciousness of signal-nucleotide polymorphism (SNP) mutations of proteins were predicted by the Polyphen-2 database. Divergent expression and overall survival (OS) of MMPs was screened by StarBase-Pan Cancer plate; and MMPs associated signaling pathways were found by Kyoto Encyclopedia Genes and Genomes. The "competing endogenous RNA (ceRNA)" network was constructed by Cytoscape software. We found that 12 specific types of SNP mutations related to 5

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Luan¹,

2022

Cell Biology International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interestingly, as telocytes release microvesicles, they are thought to be involved in contactless intercellular cross-talk [ 51 ]. In hepatocellular carcinoma, telocytes were found to promote metastasis by the production and secretion of matrix metalloproteinase 9 [ 52 ].…”

Section: Pancreas—basic Relationshipsmentioning

confidence: 99%

Neural Component of the Tumor Microenvironment in Pancreatic Ductal Adenocarcinoma

Gola

Sejda

Godlewski

et al. 2022

Cancers

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive primary malignancy of the pancreas, with a dismal prognosis and limited treatment options. It possesses a unique tumor microenvironment (TME), generating dense stroma with complex elements cross-talking with each other to promote tumor growth and progression. Diversified neural components makes for not having a full understanding of their influence on its aggressive behavior. The aim of the study was to summarize and integrate the role of nerves in the pancreatic tumor microenvironment. The role of autonomic nerve fibers on PDAC development has been recently studied, which resulted in considering the targeting of sympathetic and parasympathetic pathways as a novel treatment opportunity. Perineural invasion (PNI) is commonly found in PDAC. As the severity of the PNI correlates with a poorer prognosis, new quantification of this phenomenon, distinguishing between perineural and endoneural invasion, could feature in routine pathological examination. The concepts of cancer-related neurogenesis and axonogenesis in PDAC are understudied; so, further research in this field may be warranted. A better understanding of the interdependence between the neural component and cancer cells in the PDAC microenvironment could bring new nerve-oriented treatment options into clinical practice and improve outcomes in patients with pancreatic cancer. In this review, we aim to summarize and integrate the current state of knowledge and future challenges concerning nerve–cancer interactions in PDAC.

show abstract

“…The protocol of TCs identi cation in the liver tissue could be referenced in our previous study(Suppl Fig 1-A) [14] .…”

Section: Telocytes Identi Cationmentioning

confidence: 99%

“…Currently, the function of TCs in the development of cancers received more and more attention. In our previous study, we found that low expressions of miR-942-3p in TCs promoted matrix metalloproteinase-9(MMP9) secretion to accelerate the metastasis of HCC [14] , but the molecular mechanism was still unknown. Whether HCC cells-derived exosomes possess the contribution to impact TCs needs to be further elucidated.…”

Section: Introductionmentioning

confidence: 99%

Tumor-derived Exosomal LncRNA SNHG16 Induces Telocytes to promote metastasis of hepatocellular carcinoma via miR-942-3p/MMP9 axis

Luan

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Hepatocellular carcinoma(HCC) cells-derived exosomal LncRNA SNHG16(LncSNHG16) is highly expressed and associated with the poor overall survival of patients. Telocytes(TCs), as novel interstitial cells, possess the function to promote HCC metastasis. Therefore, whether a molecular interaction between exosomal LncSNHG16 and TCs will be revealed in our study.Methods: LncSNHG16 expression in HCC tissues and cell lines were detected, and its bioinformatics analysis was screened. Exosomes were isolated and purified from HCC cells with over-expressed/knockdown LncSNHG16 vectors and co-cultured with TCs. Then, the expression of the LncSNHG16/miR-942-3p/MMP9 axis was tested in TCs. Transwell and cell wound healing assays were designed to examine the invasion and migration of HCC cells after co-incubating with TCs. RNA immunoprecipitation(RIP) assay and Dual-Luciferase gene report were utilized to verify the binding effect of LncSNHG16, miR-942-3p, and MMP9 mRNA. In vivo, experimental animal models were established to confirm the effect of exosomal LncSNHG16-induced MMP9 expression in HCC metastasis.Results: Exosomal LncSNHG16 could be phagocytized by TCs and down-regulated miR-942-3p, which induced the targeted MMP9 up-regulation. LncSNHG16 had special binding sites with miR-942-3p in TCs to facilitate the migration of HCC cells in vitro and in vivo. Exosomal LncSNHG16 was finally supported as competing endogenous RNA of the miR-942-3p/MMP9 axis in TCs.Conclusion: Tumor-derived exosomal LncSNHG16 modulates MMP9 by competitively binding to miR-942-3p in TCs, thus promoting the metastasis of HCC.

show abstract

Telocytes promote hepatocellular carcinoma by activating the ERK signaling pathway and miR-942-3p/MMP9 axis

Cited by 14 publications

References 43 publications

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Matrix metalloproteinase gene mutations and bioinformatics of telocytes in hepatocellular carcinoma

Neural Component of the Tumor Microenvironment in Pancreatic Ductal Adenocarcinoma

Tumor-derived Exosomal LncRNA SNHG16 Induces Telocytes to promote metastasis of hepatocellular carcinoma via miR-942-3p/MMP9 axis

Contact Info

Product

Resources

About