Telomere length in the gastric mucosa after Helicobacter pylori eradication and its potential role in the gastric carcinogenesis

Tahara, Tomomitsu; Tahara, Sayumi; Tuskamoto, Tetsuya; Horiguchi, Noriyuki; Kawamura, Tomohiko; Okubo, Masaaki; Ishizuka, Takamitsu; Nagasaka, Mitsuo; Nakagawa, Yoshihito; Shibata, Tomoyuki; Kuroda, Makoto; Ohmiya, Naoki

doi:10.1007/s10238-017-0458-1

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…About 23 healthy participants without history of H. pylori infection were also enrolled who underwent upper gastroscopy for yearly checkup examination or for the complaints of abdominal discomfort (median age 53, female/male, 11/12). The study participants were partly recruited from our recent study investigating the histological gastritis, telomere length and gastric mucosal architecture in patients after successful H. pylori eradication . Fujita Health University of Medicine approved the protocol, and written informed consent was obtained from all participants.…”

Section: Methodsmentioning

confidence: 99%

“…The study participants were partly recruited from our recent study investigating the histological gastritis, telomere length and gastric mucosal architecture in patients after successful H. pylori eradication. 7,14,15 Fujita Health University of Medicine approved the protocol, and written informed consent was obtained from all participants.…”

Section: Study Populationmentioning

confidence: 99%

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DNA methylation accumulation in gastric mucosa adjacent to cancer after Helicobacter pylori eradication

Tahara

Horiguchi

et al. 2018

Intl Journal of Cancer

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Molecular irreversibleness with Helicobacter pylori (H. pylori) infection might have a role in gastric tumorigenesis after H. pylori eradication. We performed comprehensive DNA methylation profiling of gastric mucosa after H. pylori eradication with or without gastric cancer. Using four different groups of biopsies obtained from gastric body without history of H. pylori infection (Hp-), gastric body without cancer after H. pylori eradication (cancer-free body), gastric body with early gastric cancer diagnosed after H. pylori eradication (EGC body) and their paired samples from adjacent mucosa of cancer (EGC ADJ), methylation status of five candidate genes (MYOD1, SLC16A12, IGF2, RORA and PRDM5) was examined by the bisulfite pyrosequencing. An Infinium Methylation EPIC BeadChip array was also used to characterize the methylation status of greater than 850,000 CpG sites. The EGC ADJ group showed highest methylation levels of five candidate genes among the four groups of biopsies. In the gastric body (cancer-free body + EGC body), methylation levels were significantly decreased in patients with longer period after eradication, while such association was not observed in EGC ADJ group. Hyper methylated samples were associated with shorter telomere, an indicator for rapid cell turnover, and higher DNMT1 protein expression, an enzyme related to methyl transfer reaction. The genome-wide methylation analysis demonstrated strikingly higher methylation levels especially at CpG islands in the EGC ADJ group. Exclusively hypermethylated promoter CpG islands in the same group frequently coded zinc finger proteins. Our data show that DNA methylation accumulation is associated with molecular irreversibleness and gastric carcinogenesis after H. pylori eradication.

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Section: Methodsmentioning

confidence: 99%

Section: Study Populationmentioning

confidence: 99%

DNA methylation accumulation in gastric mucosa adjacent to cancer after Helicobacter pylori eradication

Tahara

Horiguchi

et al. 2018

Intl Journal of Cancer

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“…Tahara et al. demonstrated that H. pylori ‐infected gastric cancer tissues and the adjacent noncancerous tissues possessed shorter telomere length than H. pylori ‐negative gastric tissues, and that the shortening of telomere length was associated with higher expression of IL‐1B and NF‐κB …”

Section: Deregulation Of Host Functionsmentioning

confidence: 99%

“…Telomere shortening in genomic DNA is an indirect footprint, which reflects an accumulation of oxidative stress and chromosomal instability as a result of lifelong infection. Tahara et al demonstratedthat H. pylori-infected gastric cancer tissues and the adjacent noncancerous tissues possessed shorter telomere length than H. pylorinegative gastric tissues, and that the shortening of telomere length was associated with higher expression of IL-1B and NF-κB 41.…”

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confidence: 99%

Pathogenesis of Helicobacter pylori infection

2017

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Helicobacter pylori is responsible for the most commonly found infection in the world's population. It is the major risk factor for gastric cancer development. Numerous studies published over the last year provide new insights into the strategies employed by H. pylori to adapt to the extreme acidic conditions of the gastric environment, to establish persistent infection and to deregulate host functions, leading to gastric pathogenesis and cancer. In this review, we report recent data on the mechanisms involved in chemotaxis, on the essential role of nickel in acid resistance and gastric colonization, on the importance of adhesins and Hop proteins and on the role of CagPAI-components and CagA. Among the host functions, a special focus has been made on the escape from immune response, the ability of bacteria to induce genetic instability and modulate telomeres, the mechanism of autophagy and the deregulation of micro RNAs.

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“…Tahara T demonstrated that telomere length in gastric cancer or adjacent noncancerous tissues with H. pylori infection was shorter than that in gastric tissues without H. pylori infection, and the shortened telomere was associated with upregulated expression of IL-1B and NF-κB. 7 Vacuolating cytotoxin antigen (VacA) is another important virulent factor of H. pylori, and almost all H. pylori strains carry the vacA gene, but only 50%-60% of strains express VacA, and different vacA genotypes have different virulence. In acidic environment, VacA can act on the Na + -K + ATPase of target cells and disrupt the ion-protein function, damage the lysosomes and endoplasmic reticulum, eventually resulting in cellular vacuolar degeneration.…”

Section: Introductionmentioning

confidence: 99%

Advances in Helicobacter pylori vaccine research: From candidate antigens to adjuvants—A review

Yunle,

Tong,

Jiyang

et al. 2023

Helicobacter

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BackgroundHelicobacter pylori is a Gram‐negative, spiral‐shaped bacterium that infects approximately 50% of the world's population and has been strongly associated with chronic gastritis, peptic ulcers, gastric mucosa‐associated lymphoma, and gastric cancer. The elimination of H. pylori is currently considered one of the most effective strategies for the treatment of gastric‐related diseases, so antibiotic therapy is the most commonly used regimen for the treatment of H. pylori infection. Although this therapy has some positive effects, antibiotic resistance has become another clinically prominent problem. Therefore, the development of a safe and efficient vaccine has become an important measure to prevent H. pylori infection.MethodsPubMed and ClinicalTrials.gov were systematically searched from January 1980 to March 2023 with search terms—H. pylori vaccine, adjuvants, immunization, pathogenesis, and H. pylori eradication in the title and/or abstract of literature. A total of 5182 documents were obtained. Based on the principles of academic reliability, authority, nearly publicated, and excluded the similar documents, finally, 75 documents were selected, organized, and analyzed.ResultsMost of the candidate antigens used as H. pylori vaccines in these literatures are whole‐cell antigens and virulence antigens such as UreB, VacA, CagA, and HspA, and the main types of vaccines for H. pylori are whole bacteria vaccines, vector vaccines, subunit vaccines, nucleic acid vaccines, epitope vaccines, etc. Some vaccines have shown good immune protection in animal trials; however, few vaccines show good in clinical trials. The only H. pylori vaccine passed phase 3 clinical trial is a recombinant subunit vaccine using Urease subunit B (UreB) as the vaccine antigen, and it shows good prophylactic effects. Meanwhile, the adjuvant system for vaccines against this bacterium has been developed considerably. In addition to the traditional mucosal adjuvants such as cholera toxin (CT) and E. coli heat labile enterotoxin (LT), there are also promising safer and more effective mucosal adjuvants. All these advances made safe and effective H. pylori vaccines come into service as early as possible.ConclusionsThis review briefly summarized the advances of H. pylori vaccines from two aspects, candidates of antigens and adjuvants, to provide references for the development of vaccine against this bacterium. We also present our prospects of exosomal vaccines in H. pylori vaccine research, in the hope of inspiring future researchers.

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Telomere length in the gastric mucosa after Helicobacter pylori eradication and its potential role in the gastric carcinogenesis

Cited by 6 publications

References 24 publications

DNA methylation accumulation in gastric mucosa adjacent to cancer after Helicobacter pylori eradication

DNA methylation accumulation in gastric mucosa adjacent to cancer after Helicobacter pylori eradication

Pathogenesis of Helicobacter pylori infection

Advances in Helicobacter pylori vaccine research: From candidate antigens to adjuvants—A review

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