Temperatibacter marinus gen. nov., sp. nov., a mesophilic bacterium isolated from surface seawater and description of Temperatibacteraceae fam. nov. in the class Alphaproteobacteria

Abstract

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 5-11 T , was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. The strain exhibited a narrow growth temperature range of 20-30 6C. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the order Kordiimonadales in the class Alphaproteobacteria and was related most closely to the genus Kordiimonas (up to 91.2 % similarity to the type strains of species of the genus) but branched deeply from species of Kordiimonas. The major fatty acids were iso-C 17 : 1 v9c, iso-C 15 : 0 , and C 16 : 1 v7c and/or iso-C 15 : 0 2-OH. Ubiquinone-10 (Q-10) was detected as the sole isoprenoid quinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. Although strains of Kordiimonas have been shown to contain unidentified glycolipids, they were not detected from strain 5-11 T . The DNA G+C content of strain 5-11 T was 44.3 mol%, a value that was lower than those of strains of Kordiimonas (50-58 mol%) and was relatively low for the members of the class Alphaproteobacteria. On the basis of phenotypic, genotypic and chemotaxonomic data, it is proposed that strain 5-11 T represents a novel species of a new genus, Temperatibacter marinus gen. nov., sp. nov., within a new family Temperatibacteraceae fam. nov. The type strain of Temperatibacter marinus is 5-11 T (5NBRC 110045 T 5LMG 28278 T ).

Kordiimonas lipolytica sp. nov., isolated from seawater

“…All species have mesophilic characteristics for growth temperature (optimum !30 C), except K. aquimaris (optimum 20 C) (Yang et al, 2013). Phylogenetic analysis revealed the genus Kordiimonas to be closely related to the genera Eilatimonas and Temperatibacter (Teramoto & Nishijima, 2014). They can be distinguished from other groups within the class Alphaproteobacteria by their cellular fatty acid composition, having high contents of iso-and anteiso-branched fatty acids (Paramasivam et al, 2013;Teramoto & Nishijima, 2014;Xu et al, 2014).…”

“…Phylogenetic analysis revealed the genus Kordiimonas to be closely related to the genera Eilatimonas and Temperatibacter (Teramoto & Nishijima, 2014). They can be distinguished from other groups within the class Alphaproteobacteria by their cellular fatty acid composition, having high contents of iso-and anteiso-branched fatty acids (Paramasivam et al, 2013;Teramoto & Nishijima, 2014;Xu et al, 2014). A novel Kordiimonas strain, M41 T , was isolated from a surface seawater sample collected from the western Pacific Ocean and was subjected to taxonomic study using a polyphasic approach.…”

Gimibacter soli gen. nov. sp. nov., isolated from mangrove soil and insight into its ecological distribution and metabolic potential

“…Differential characteristics between strain 6D33 T and the reference type strains +, Positive; −, negative; nd, no data; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; AL, aminolipid; APL, aminophospholipid; GL, glycolipid; PL, phospholipid; L, lipid. The data of Temperatibacter marinus 5-11 T were obtained from the original study [1]. revealed that 16S rRNA gene sequence of strain 6D33 T matched only 261 out of 500 048 datasets (Table S4), comprising mainly 107 marine datasets, 37 soil datasets, 28 Triticum aestivum datasets from salinized soil irrigated by diluted seawater and 26 sediment metagenome datasets with a sequence similarity threshold of 97 %.…”

“…The family Temperatibacteraceae was proposed by Teramoto and Nishijima in 2014 [1] and belongs to the order Kordiimonadales of the class Alphaproteobacteria . At the time of writing, the family Temperatibacteraceae comprises three genera, Eilatimonas [2], Temperatibacter [1] and Kordiimonas [3]. The first two genera currently include only one species, Eilatimonas milleporae and Temperatibacter marinus .…”

“…The genus Kordiimonas currently consists of nine species with correct names validly published under the International Code of Nomenclature of Prokaryotes [4], including Kordiimonas aestuarii [5], Kordiimonas aquimaris [6], Kordiimonas gwangyangensis (type species) [3], Kordiimonas lacus [7], Kordiimonas laminariae [8], Kordiimonas lipolytica [9], Kordiimonas marina [8], Kordiimonas pumila [10] and Kordiimonas sediminis [11]. The family Temperatibacteraceae bacteria are often isolated from marine environments, such as the hydrocoral Millepora dichotoma [2], seawater [1, 6, 7] and sediments [3, 5, 8, 10]. Several studies demonstrated that some members of this family could degrade carbazole [12] and diesel oil [13]; some may play important roles in the biogeochemical cycles of carbon, nitrogen, sulphur, phosphorus and others [14].…”