Temperature dependence analysis of the NIR spectra of liquid water confirms the existence of two phases, one of which is in a coherent state

Abstract

Background: isosbestic (equal absorption) points in the IR and NIR spectra of liquid water are a well-known feature and they witness the existence of two populations of oscillators in the probed system. Despite it is a well-known experimental fact, in the mainstream molecular dynamics approach the proposed theoretical explanations for it are not able to elucidate which is the physical reason why such a "cut-off frequency" (at the isosbestic point) does exist. Methods: We investigate pure Milli-Q water on increasing the temperature in the vis-NIR range (400-2500 nm). We specifically payed attention to the first overtone region (1300-1600 nm) of the OH-bond stretching-mode where an isosbestic point has been observed. Results: A second derivative analysis clearly shows two modes, which can be assigned to water molecules involved in different "hydrogen bonding" configurations whose relative abundance is controlled by the temperature. We have also observed that the ratio of these modes follows a van't Hoff behavior supporting that their energy difference (energy gap) is independent on the temperature. Furthermore, a log-log plot shows a scale invariance of the population ratio with respect to the perturbation (temperature), confirming the existence of a long-range correlated dynamics in the liquid. Conclusions: We show that the two phases differences between energy and entropy estimated from the experimental data can be compared with the prediction of Quantum Electro Dynamics (QED) showing a remarkable agreement.

Aquaphotomics Reveals Subtle Differences between Natural Mineral, Processed and Aged Water Using Temperature Perturbation Near-Infrared Spectroscopy

“…An experiment was conducted to discriminate between the various types of waters using NIR spectra in the region of the first overtone of water (1300-1600 nm). NIR absorbance spectra of different waters at temperatures varying from 30 to 60 • C (Figure S1), show that the peak of the first overtone spectra experiences a shift towards lower wavelengths ("blue shift") [3,24] from 1450 to 1428 nm with an increasing temperature, with an isosbestic point around 1442 nm (magnified view in Figure S1), which is consistent with the well-known influence of temperature on water spectra [5,25].…”

Effects of Resonant Electromagnetic Fields on Biofilm Formation in Pseudomonas aeruginosa

“…Only clusters stabilized by surfaces have lifetimes long enough to store and release energy from the ambience [11,12]. Experimental evidence of this theory has been supported by experimental findings using various spectroscopic methods, including infrared and near-infrared spectroscopy and X-ray studies of supercooled water [13][14][15][16].…”

Another Look at the Water Phases that Exist Under Room Conditions

“…Many physical quantities exhibit behavior that suggests the presence of two states in liquid water. For example, infrared and Raman spectra, as well as the presence of an isobestic point in the Raman spectra, are considered as a clear sign confirming the model of a mixture of two phases [31]. According to Preparata et all [32], liquid water is a two-component liquid system consisting of a coherent phase (about 40% of the total volume at room temperature) and an incoherent phase.…”

“…However, after half a century, the opinion that liquid water is two-phase resurfaced. According to modern data, the simplest model of the structure of liquid water assumes the existence of two separate microscopic phases [26][27][28][29][30][31]. Many physical quantities exhibit behavior that suggests the presence of two states in liquid water.…”