Temperature-dependence of stress-induced hepatic autophagy

Salas, Margarita; Tuchweber, Béatriz; Kourounakis, Panos N.; Selye, Hans

doi:10.1007/bf01946531

Cited by 17 publications

(11 citation statements)

References 7 publications

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“…These results confirmed the impact of oxygen delivery during preservation on the maintenance of graft ultrastructural morphology. However, since mitochondria1 swelling increased during preservation in a time-dependent manner by each method, mitochondria may be strongly influenced not only by hypoxia but also by hypothermic stress as was seen in the liver model [13].…”

Section: Resultsmentioning

confidence: 92%

Ultrastructural analyses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solution

Iwanaga¹,

Suzuki²,

Okada³

et al. 2002

Transplant Int

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U I t rast ruct u ra I a na I yses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solutionAbstract The two-layer cold storage method (TLM) using University of Wisconsin (UW) solution supplies sufficient oxygen to pancreatic grafts during preservation and extends pancreas preservation time to up to 96h in the canine model. Simple cold storage in UW (UWM) on the other hand, preserves canine pancreas grafts for up to 72h by preventing cell swelling, mainly because of its high osmotic pressure. The aim of this study is to analyze morphologically dog pancreatic grafts preserved by these two methods with their different mechanisms. Immediately after preservation of canine pancreata by TLM for 72h and 96h (group 1 and group 3, respectively), and by UWM for 72h and 96h (group 2 and group 4, respectively), tissue ATP levels were determined using high-performance liquid chromatography (HPLC), and detailed morphological analyses of intragraft components were performed using light-and electron microscopy. The mean areas of one mitochondrion and rough endoplasmic reticulum (RER) vacuolization were calculated by computer-graphic analyses using NIH image 1.62f soft. The tissue ATP levels were significantly higher in groups 1 and 3 than groups 2 and 4 (P < 0.05).Light microscopy demonstrated no marked difference among the 4 groups. By electron microscopy however, mitochondria1 swelling and RER vacuolization were observed in acinar cells to various extents in the 4 groups. They were significantly more evident in group 2 than group 1 (P < 0.05), and in group 4 than group 3 ( P < 0.05).In conclusion, TLM demonstrated excellent protection of intracellular organelles, mitochondria, and RER, up to 72-96h. Well-maintained graft ATP levels in TLM groups may result in maintaining the integrity of intracellular organelle membranes as well as cellular membranes. Keywords

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Section: Resultsmentioning

confidence: 92%

Ultrastructural analyses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solution

Iwanaga¹,

Suzuki²,

Okada³

et al. 2002

Transplant Int

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“…Immunohistochemistry showed the presence of a number of small accumulations of LC3 co-localized with cytochrome C in the cytoplasm of these hepatocytes (Panel C in S2 Fig). Salas et al found that hypothermia was a decisive element in the production of hepatic autophagy in rats [78,79]. In addition, the appearances of swollen mitochondria in the hepatocyte of mice under conditions of hypothermia have suggested that the damage to mitochondria is induced by the hypothermic condition itself [78].…”

Section: Discussionmentioning

confidence: 99%

“…Salas et al found that hypothermia was a decisive element in the production of hepatic autophagy in rats [78,79]. In addition, the appearances of swollen mitochondria in the hepatocyte of mice under conditions of hypothermia have suggested that the damage to mitochondria is induced by the hypothermic condition itself [78]. Extended times of HMP may also be subject to particular disadvantages and limitations with regard to endoplasmic stress [80,81].…”

Section: Discussionmentioning

confidence: 99%

The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation

et al. 2017

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The effects of warm machine perfusion preservation of liver grafts donated after cardiac death on the intracellular three-dimensional ultrastructure of the organelles in hepatocytes remain unclear. Here we analyzed comparatively the ultrastructure of the endomembrane systems in porcine hepatocytes under warm ischemia and successive hypothermic and midthermic machine perfusion preservation, a type of the warm machine perfusion. Porcine liver grafts which had a warm ischemia time of 60 minutes were perfused for 4 hours with modified University of Wisconsin gluconate solution. Group A grafts were preserved with hypothermic machine perfusion preservation at 8°C constantly for 4 hours. Group B grafts were preserved with rewarming up to 22°C by warm machine perfusion preservation for 4 hours. An analysis of hepatocytes after 60 minutes of warm ischemia by scanning electron microscope revealed the appearance of abnormal vacuoles and invagination of mitochondria. In the hepatocytes preserved by subsequent hypothermic machine perfusion preservation, strongly swollen mitochondria were observed. In contrast, the warm machine perfusion preservation could preserve the functional appearance of mitochondria in hepatocytes. Furthermore, abundant vacuoles and membranous structures sequestrating cellular organelles like autophagic vacuoles were frequently observed in hepatocytes after warm machine perfusion preservation. In conclusion, the ultrastructure of the endomembrane systems in the hepatocytes of liver grafts changed in accordance with the temperature conditions of machine perfusion preservation. In addition, temperature condition of the machine perfusion preservation may also affect the condition of the hepatic graft attributed to autophagy systems, and consequently alleviate the damage of the hepatocytes.

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“…It is believed that the stress-induced changes in liver appear to be directly propor tional to the degree of hypothermia elicited by the stressing agents [Salas et al, 1977], Therefore, fasting a stressor also caused a significant depletion in hepatic glycogen content of rat. Rao [1979] observed a similar degree of depletion of muscle glycogen in starved lizards of young and old age groups.…”

Section: Resultsmentioning

confidence: 99%

Age-Related Responses of Hepatic Glucose Uptake to Starvation and Cold Stress in Male Garden Lizards

Jena

Patnaik²

1990

Gerontology

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Temperature-dependence of stress-induced hepatic autophagy

Cited by 17 publications

References 7 publications

Ultrastructural analyses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solution

Ultrastructural analyses of pancreatic grafts preserved by the two-layer cold-storage method and by simple cold storage in University of Wisconsin solution

The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation

Age-Related Responses of Hepatic Glucose Uptake to Starvation and Cold Stress in Male Garden Lizards

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