Temperature-dependent Azide Sensitivity of Growth and ATPase Activity in the Facultative Thermophile, Bacillus coagulans

Jones, Martin V.; Spencer, WN; Edwards, C.

doi:10.1099/00221287-130-1-95

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Hemes O and D widely spread in most gram negative bacteria were not detected in those membranes [16]. Cytochrome bd induction was reported in B. pseudofirmus OF4 under growth on glucose at neu tral pH or under growth on mineral medium at pH 10.5 up to the stationary phase of growth [17], in B. subtilis under growth on glucose [18], and in thermophilic species Bacillus stearothermophilus [19] and Bacillus coag ulans under the simultaneous temperature increase and oxygen decrease in the growth medium [20]. Cytochrome o was found in membranes of some thermophilic species, B. stearothermophilus [21] and Bacillus PS3 [22].…”

Section: Membrane Cytochromesmentioning

confidence: 94%

Energetics of alkalophilic representatives of the genus Bacillus

Muntyan

Popova

Bloch

et al. 2005

Biochemistry (Moscow)

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Cytochrome and lipid composition of membranes is considered as the attributes required for adaptation of the alkalophiles to alkaline conditions. Respiratory chains of alkalophilic representatives of the genus Bacillus are discussed. Special attention is paid to the features of the Na(+)-cycle of these bacteria and to the features determining halo- and alkalotolerant phenotype, which have been reported due to recent achievements in genomics.

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Section: Membrane Cytochromesmentioning

confidence: 94%

Energetics of alkalophilic representatives of the genus Bacillus

Muntyan

Popova

Bloch

et al. 2005

Biochemistry (Moscow)

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“…However, inhibition by sodium azide is dependent on growth and assay temperature, the ATPase from cells grown at 55°C being least sensitive to this inhibitor. cally in tryptone/yeast extract/glucose (TYG) broth in conical flasks as previously described [4,5]. These temperature-dependent modifications could reflect changes in BF0, perhaps by alteration of membrane lipids, or molecular or subunit differences in BF 1.…”

Section: Introductionmentioning

confidence: 99%

“…These temperature-dependent modifications could reflect changes in BF0, perhaps by alteration of membrane lipids, or molecular or subunit differences in BF 1. The enzyme was assayed by a proton release method as described previously [5,7,8]. cally in tryptone/yeast extract/glucose (TYG) broth in conical flasks as previously described [4,5].…”

Section: Introductionmentioning

confidence: 99%

Properties of the ATP phosphohydrolase of the facultative thermophile Bacillus coagulans

Ball

1985

FEMS Microbiology Letters

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The thermostability of the ATP phosphohydrolase of the facultative thermophile Bacillus coagulans has been investigated. Fractionation of disintegrated cell suspensions by differential centrifugation revealed a similar distribution of enzyme activity irrespective of growth temperature. Most of the activity was located in the membrane fraction. Thermostability of solubilized (BF1) preparation from cells grown at 37°C or 55°C was similar, but membrane‐bound BF0BF1 from 37°C‐grown cells was inactivated at lower temperatures than that from 55°C‐grown cells. Inhibition of the membrane‐bound (BF0BF1)ATPase by 4‐chloro‐7‐nitro‐benzofuran (NbfCl) and quercetin, which both act on the BF1 portion of the enzyme, was different from that seen with the soluble (BF1) enzyme. The results show that some modification of BF1 must occur when the enzyme is membrane‐bound.

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Effect of low pH start-up on continuous mixed-culture lactic acid fermentation of dairy effluent

Choi

Kim

Lee

2016

Appl Microbiol Biotechnol

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Mixed-culture fermentation that does not require an energy-intensive sterilization process is a viable approach for the economically feasible production of lactic acid (LA) due to the potential use of organic waste as feedstock. This study investigated mixed-culture LA fermentation of whey, a high-strength organic wastewater, in continuous mode. Variations in the hydraulic retention time (HRT) from 120 to 8 h under different pH regimes in two thermophilic reactors (55 °C) were compared for their fermentation performance. One reactor was maintained at a low pH (pH 3.0) during operation at HRTs of 120 to 24 h and then adjusted to pH 5.5 in the later phases of fermentation at HRTs of 24 to 8 h (R1), while the second reactor was maintained at pH 5.5 throughout the experiment (R2). Although the LA production in R1 was negligible at low pH, it increased dramatically after the pH was raised to 5.5 and exceeded that in R2 when stabilized at HRTs of 8 and 12 h. The maximum yield (0.62 g LA/g substrate fed as the chemical oxygen demand (COD) equivalent), the production rate (11.5 g/L day), and the selectivity (95 %) of LA were all determined at a 12-h HRT in R1. Additionally, molecular and statistical analyses revealed that changes in the HRT and the pH significantly affected the bacterial community structure and thus the fermentation characteristics of the experimental reactors. Bacillus coagulans was likely the predominant LA producer in both reactors. The overall results suggest that low pH start-up has a positive effect on yield and selectivity in mixed-culture LA fermentation.

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Temperature-dependent Azide Sensitivity of Growth and ATPase Activity in the Facultative Thermophile, Bacillus coagulans

Cited by 4 publications

References 14 publications

Energetics of alkalophilic representatives of the genus Bacillus

Energetics of alkalophilic representatives of the genus Bacillus

Properties of the ATP phosphohydrolase of the facultative thermophile Bacillus coagulans

Effect of low pH start-up on continuous mixed-culture lactic acid fermentation of dairy effluent

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