Temperature distribution and local heat flux in the unidirectional freezing of antifreeze-protein solution

Hagiwara, Yoshimichi; Yamamoto, Daichi

doi:10.1016/j.ijheatmasstransfer.2012.01.024

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…Thus, many experiments on unidirectional freezing have been carried out [ 12 – 16 ]. From the results in [ 13 , 15 , 16 ], in the case of AF(G)P solutions, it was found that the decrease in the temperature at the most advanced point of the serrated interface is consistent with the freezing point drop measured with osmometers, although the interface temperature depended on the interface velocity.…”

Section: Introductionmentioning

confidence: 52%

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Ice Growth Inhibition in Antifreeze Polypeptide Solution by Short-Time Solution Preheating

Nishi¹,

Miyamoto²,

Waku³

et al. 2016

PLoS ONE

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The objective of this study is to enhance the inhibition of ice growth in the aqueous solution of a polypeptide, which is inspired by winter flounder antifreeze protein. We carried out measurements on unidirectional freezing of the polypeptide solution. The thickness of the solution was 0.02 mm, and the concentration of polypeptide was varied from 0 to 2 mg/mL. We captured successive microscopic images of ice/solution interfaces, and measured the interface velocity from the locations of tips of the pectinate interface in the images. We also simultaneously measured the temperature by using a small thermocouple. The ice/solution interface temperature was defined by the temperature at the tips. It was found that the interface temperature was decreased with an increasing concentration of polypeptide. To try varying the activity of the polypeptide, we preheated the polypeptide solution and cooled it before carrying out the measurements. Preheating for 1–5 hours was found to cause a further decrease in the interface temperature. Furthermore, wider regions of solution and ice with inclined interfaces in the pectinate interface structure were observed, compared with the case where the solution was not preheated. Thus, the ice growth inhibition was enhanced by this preheating. To investigate the reason for this enhancement, we measured the conformation and aggregates of polypeptide in the solution. We also measured the local concentration of polypeptide. It was found that the polypeptide aggregates became larger as a result of preheating, although the polypeptide conformation was unchanged. These large aggregates caused both adsorption to the interface and the wide regions of supercooled solution in the pectinate interface structure.

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Section: Introductionmentioning

confidence: 52%

“…We used fluorescence microscopy to measure the local concentration of polypeptide. This method is the same as that used in our previous studies [ 15 , 16 ]. The amino-base side-chain of the C-terminus of the polypeptide was tagged with fluorescein isothiocyanate (FITC) via a linker.…”

Section: Methodsmentioning

confidence: 99%

Ice Growth Inhibition in Antifreeze Polypeptide Solution by Short-Time Solution Preheating

Nishi¹,

Miyamoto²,

Waku³

et al. 2016

PLoS ONE

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“…Several attempts were done to formulate general description or numerical algorithms to describe the freezing on translational temperature gradient stage. For example, uni-dimensional temperature distribution was calculated numerically for broad freezing velocity range [17,18,19] and an algorithm for calculation of three-dimensional temperature field in case of slow freezing velocities was proposed [20]. Such general solutions require heavy computations and do not provide a predictable observables that could be directly accessed through experiment.…”

Section: Introductionmentioning

confidence: 99%

Heat flux balance description of unidirectional freezing and melting dynamics on a translational temperature gradient stage

Chasnitsky¹,

Yashunsky²,

Braslavsky³

2021

International Journal of Thermal Sciences

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Directional solidification occurs in industrial and natural processes, such as freeze-casting, metal processing, biological cryopreservation and freezing of soils. Translational temperature gradient stage allows to control the process of directional solidification and to visualise it with optical microscope. In this stage freezing velocity and temperature gradient are decoupled and are independently controlled. Here we study the dynamics of the phase transition interface in thin water samples using translational temperature gradient stage. We follow position of the ice-water interface with optical microscopy and compare it to solution of one dimensional Stefan problem in the low velocity limit. We find an agreement between experimental observations and theoretical predictions for constant velocity and during acceleration of the ice front. This work presents a practical framework for analysis and design of experiments on a translational temperature gradient stage.

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“…In the case of type (ii) measurements, there are several other experimental methods that can help to elucidate the activities of AF(G)P: Wilson et al [6] developed an automated lag-time apparatus where a 200 mm 3 sample solution of winter flounder AFP was held in a glass tube, and they measured the nucleation rate in the tube. Coger et al [7], Furukawa et al [8], Butler [9] Hagiwara and Yamamoto [10], Hagiwara and Aomatsu [11] and Miyamoto et al [12] carried out experiments on the unidirectional freezing of AF(G)P solutions in narrow spaces between two glass plates. They measured the velocities and morphologies of the ice/solution interfaces, the concentration of solutes, temperature distribution and the dimension of AFP aggregates.…”

Section: Introductionmentioning

confidence: 99%

Effects of Winter Flounder Antifreeze Protein on the Growth of Ice Particles in an Ice Slurry Flow in Mini-Channels

et al. 2019

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The control of ice growth in ice slurry is important for many fields, including (a) the cooling of the brain during cardiac arrest, (b) the storage and transportation of fresh fish and fruits, and (c) the development of distributed air-conditioning systems. One of the promising methods for the control is to use a substance such as antifreeze protein. We have observed and report here growth states of ice particles in both quiescent and flowing aqueous solutions of winter flounder antifreeze proteins in mini-channels with a microscope. We also measured ice growth rates. Our aim was to improve the levels of ice growth inhibition by subjecting the antifreeze protein solution both to preheating and to concentrating by ultrafiltration. We have found that the ice growth inhibition by the antifreeze protein decreased in flowing solutions compared with that in quiescent solutions. In addition, unlike unidirectional freezing experiments, the preheating of the antifreeze protein solution reduced the ice growth inhibition properties. This is because the direction of flow, containing HPLC6 and its aggregates, to the ice particle surfaces can change as the ice particle grows, and thus the probability of interaction between HPLC6 and ice surfaces does not increase. In contrast to this, ultrafiltration after preheating the solution improved the ice growth inhibition. This may be due to the interaction between ice surfaces and many aggregates in the concentrates.

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Temperature distribution and local heat flux in the unidirectional freezing of antifreeze-protein solution

Cited by 9 publications

References 18 publications

Ice Growth Inhibition in Antifreeze Polypeptide Solution by Short-Time Solution Preheating

Ice Growth Inhibition in Antifreeze Polypeptide Solution by Short-Time Solution Preheating

Heat flux balance description of unidirectional freezing and melting dynamics on a translational temperature gradient stage

Effects of Winter Flounder Antifreeze Protein on the Growth of Ice Particles in an Ice Slurry Flow in Mini-Channels

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