Unfortunately, it is difficult for these new materials to have combined transparency, low modulus, high toughness, selfrecovery, and high sensitivity.Conductive hydrogels are new softwet materials that can transform external stress stimuli (e.g., tension or pressure) into detectable digital electrical signals (e.g., current, voltage, or capacitance) and are ideal candidate materials for e-skin. Traditional single-network (SN) conductive hydrogels have a low elasticity modulus, good self-recovery, and fatigue resistance properties; however, they lack toughness. [7][8][9] Toughened nanocomposite (NC) hydrogels and hybrid hydrogels exhibit high toughness but have high elasticity moduli. However, the biotoxicity caused by chemical cross-linking agents during hydrogel preparation is another factor limiting the application of conductive hydrogels in vivo.Double network (DN) hydrogels, especially fully physically cross-linked DN hydrogels, can address these disadvantages by elaborately designing the first and second networks, owing to their peculiar energy dissipation mechanism for enhancing their mechanical properties. By introducing dynamically reversible physical bonds or interactions (e.g., hydrogen bonds, [10,11] metal ion complexation, [12,13] hydrophobic association, [14,15] or polymer chain entanglement [16,17] ) into both networks, fully physically cross-linked DN hydrogels can be fabricated, endowing the hydrogel with excellent self-recovery, anti-fatigue, and adhesion properties. Zheng et al. reported a series of fully physically cross-linked DN hydrogels based on reversible hydrogen bond and ion complexation, including Agar/poly(acrylic acid) -Fe 3+ (Agar/PAAc-Fe 3+ ) gels, [18] Agar/poly(N-hydroxyethyl acrylamide) (Agar/pHEAA), [10] and Gelatin/pHEAA. [19] Recently, Gao et al. constructed a fully physically cross-linked alginate (SA)/poly(acrylamide-acrylic acid -octadecyl methacrylate)-Fe 3+ (SAp(AAm-AAc-OMA)-Fe 3+ ) DN hydrogel using hydrophobic association and ionic coordination. [20] The hydrogel exhibited a tensile strength of 3.31 MPa, prominent anti-fatigue properties, and a self-recovery efficiency of ≈100% after 5 min.DNA is a biological macromolecule with a double-helix structure that can be reversibly switched by external stimuli, Electronic skin (e-skin) is widely studied for its ability to detect physiological information and provide feedback through electrical signals. Biocompatible stimulus-responsive DNA-based hydrogels exhibit high sensitivity, which makes them outstanding candidates for biomedical applications. However, several disadvantages, such as weak mechanical properties, expensive manufacturing costs, cumbersome double chain design, and difficulty obtaining bulk size, seriously limit their practical application. Based on the double network (DN) strategy, a universal, low-cost, and high-toughness deoxyribonucleic acid/poly(N-hydroxyethyl acrylamide) (DNA/pHEAA) DN hydrogel is designed. The transparent hydrogel shows tensile stress/strain of 0.96 ± 0.043 MPa/2537.55 ± 23.24%, rapid se...