Temperature effect on chiral recognition of some amino acids with molecularly imprinted polymer filled capillary electrochromatography

Approaches to molecular imprinting based selectivity in capillary electrochromatography

“…The selectivity of these columns were low and only pentamidine showed a pH-dependent retention that might originate from imprinting. One reason for the low selectivity could be the use of the highly polar Enantiomers of amino acids L-Phenylalanine anilide [11,14,16] Enantiomers of amino acids L-Phenylalanine [14] Enantiomers of amino acids L-Dansyl-leucin, L-dansylphenylalanine [15] Silicate entrapped MIP particles…”

“…One can also expect that air bubble formation in the gel perpetually will ruin the capillary column because there are no means to regenerate the column. The above method was further developed and modified to use only polyacrylamide gel frits to hold a ground MIP particle slurry in a defined section of the capillary [14][15][16]. To facilitate on-column detection the capillary was then connected to an empty capillary via a Teflon sleeve (Fig.…”

“…Lin et al [16] studied the effect of polymerization temperature on the chiral recognition of three amino acids. They used either UV-initiated polymerization or thermal initiation at 47C, 407C or 607C.…”

“…In this study, the optimal organic modifier was acetonitrile when added in 10-20 vol%. In later works by the same group a high content, i.e., 70 vol% [18], 80 vol% [16,19] and 90 vol% of acetonitrile [14], was described as the optimal amount of organic modifier in the electrolyte.…”

Enantiomer Separation by Capillary Electrochromatography Using Fritless Packed Columns

“…[9][10][11] In 1997, Nilsson et al used fritless packed column technology to prepare MIP columns for CEC enantioseparation. [12][13][14] The prepolymerization mixture comprised imprint molecule (β-blocker, (R)-propranolol or (S)-metoprolol), functional (methacrylic acid, MAA)) and crosslinking (trimethylolpropane trimethacylate, TRIM) monomers, radical initiator (2,2′-azobis(isobutyronitrile, AIBN) and solvent (toluene).…”