Eliminating cracks is a big challenge for the selective laser melting (SLM) process of low-weldable Nickel-based superalloy. In this work, three scanning strategies of the snake, stripe partition, and chessboard partition were utilized to prepare René 104 Ni-based superalloy, of which the cracking behavior and the residual stress were investigated. The results showed that the scanning strategies had significant effects on the cracking, residual stress, and relative density of the SLMed René 104 superalloy. The scanning strategies with more partitions boosted the emergence of cracks, as high-density cracks occurred in these samples. The overlapping zone (OZ) of the scanning partition was also susceptible to cracking, which increased the size, number, and density of the cracks. The cracking performance was relatively moderate in the snake-scanned samples, while that in the chessboard-partition-scanned samples was the most severe. It is concluded that the partition scanning strategies induced more cracks in the SLMed René 104 superalloy, of which the residual stress was apparently reduced. Therefore, it is necessary to design scanning strategies with optimized scanning partitions and overlaps to avoid cracking and acquire a high-quality, near fully dense, low-weldable Nickel-based superalloy using SLM.