Temperature-Induced Changes in the Fatty Acid Composition of the Cyanobacterium, <i>Synechocystis</i> PCC6803

Wada, Hajime; Murata, Norio

doi:10.1104/pp.92.4.1062

Cited by 175 publications

(141 citation statements)

References 29 publications

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“…The drastic decrease observed in state transitions amplitude during cold shock for the tropical and mid-latitude strains generally arose before the PBS uncoupling in these strains, indicating that the decline in state transition during the first few days was not directly related to PBS dismantling (Figures 2b and 3a). These early perturbations of the state transition process might originate in the cold-induced changes in the fluidity of the thylakoidal membranes, as low temperature is well known to induce membrane stiffening (Wada and Murata, 1990;Murata and Los, 1997;Mikami and Murata, 2003). Later during the cold period, PBS dismantling likely contributed to the complete inhibition of state transitions.…”

Section: Discussionmentioning

confidence: 99%

Connecting thermal physiology and latitudinal niche partitioning in marine Synechococcus

Pittera

Humily

Thorel³

et al. 2014

The ISME Journal

138

171

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Marine Synechococcus cyanobacteria constitute a monophyletic group that displays a wide latitudinal distribution, ranging from the equator to the polar fronts. Whether these organisms are all physiologically adapted to stand a large temperature gradient or stenotherms with narrow growth temperature ranges has so far remained unexplored. We submitted a panel of six strains, isolated along a gradient of latitude in the North Atlantic Ocean, to long-and short-term variations of temperature. Upon a downward shift of temperature, the strains showed strikingly distinct resistance, seemingly related to their latitude of isolation, with tropical strains collapsing while northern strains were capable of growing. This behaviour was associated to differential photosynthetic performances. In the tropical strains, the rapid photosystem II inactivation and the decrease of the antioxydant b-carotene relative to chl a suggested a strong induction of oxidative stress. These different responses were related to the thermal preferenda of the strains. The northern strains could grow at 10 1C while the other strains preferred higher temperatures. In addition, we pointed out a correspondence between strain isolation temperature and phylogeny. In particular, clades I and IV laboratory strains were all collected in the coldest waters of the distribution area of marine Synechococus. We, however, show that clade I Synechococcus exhibit different levels of adaptation, which apparently reflect their location on the latitudinal temperature gradient. This study reveals the existence of lineages of marine Synechococcus physiologically specialised in different thermal niches, therefore suggesting the existence of temperature ecotypes within the marine Synechococcus radiation.

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Section: Discussionmentioning

confidence: 99%

Connecting thermal physiology and latitudinal niche partitioning in marine Synechococcus

Pittera

Humily

Thorel³

et al. 2014

The ISME Journal

138

171

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“…Two genes downregulated in the wild-type, desA (slr1350) and desD (sll0262), encode acyl-lipid desaturases that introduce double bonds at defined positions in fatty acids that form membrane glycerolipids (Wada & Murata, 1990). The presence of double bonds in membrane lipids has been implicated in protecting PSII from photoinhibiton under stress conditions (Allakhverdiev et al, 2001;Tasaka et al, 1996).…”

Section: The Absence Of Hik31 Alters Transcriptional Response To Low mentioning

confidence: 99%

Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses

2011

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We have investigated the response of the cyanobacterium Synechocystis sp. PCC 6803 during growth at very low O 2 concentration (bubbled with 99.9 % N 2 /0.1 % CO 2 ). Significant transcriptional changes upon low-O 2 incubation included upregulation of a cluster of genes that contained psbA1 and an operon that includes a gene encoding the two-component regulatory histidine kinase, Hik31. This regulatory cluster is of particular interest, since there are virtually identical copies on both the chromosome and plasmid pSYSX. We used a knockout mutant lacking the chromosomal copy of hik31 and studied differential transcription during the aerobiclow-O 2 transition in this DHik31 strain and the wild-type. We observed two distinct responses to this transition, one Hik31 dependent, the other Hik31 independent. The Hik31-independent responses included the psbA1 induction and genes involved in chlorophyll biosynthesis. In addition, there were changes in a number of genes that may be involved in assembling or stabilizing photosystem (PS)II, and the hox operon and the LexA-like protein (Sll1626) were upregulated during low-O 2 growth. This family of responses mostly focused on PSII and overall redox control. There was also a large set of genes that responded differently in the absence of the chromosomal Hik31. In the vast majority of these cases, Hik31 functioned as a repressor and transcription was enhanced when Hik31 was deleted. Genes in this category encoded both core and peripheral proteins for PSI and PSII, the main phycobilisome proteins, chaperones, the ATP synthase cluster and virtually all of the ribosomal proteins. These findings, coupled with the fact that DHik31 grew better than the wild-type under low-O 2 conditions, suggested that Hik31 helps to regulate growth and overall cellular homeostasis. We detected changes in the transcription of other regulatory genes that may compensate for the loss of Hik31. We conclude that Hik31 regulates an important series of genes that relate to energy production and growth and that help to determine how Synechocystis responds to changes in O 2 conditions.

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“…These results suggested that ω-3 Des, which converts LA to ALA, is regulated by temperature. Whereas cold-induced expression of ω-3 Des gene at mRNA level have been found in algae and many plants (Wada and Murata 1990;Shi et al, 2011;Zhang et al, Science Publications AJBB 2011; Takemura et al, 2012) and it is most sensitive to changes in temperature compared to other desaturases such as ∆9, ∆12 and ∆6 Des gene (Sakamoto et al, 1994). Therefore, it is suggested that ω-3 Des gene in Mt.…”

Section: Molecular Switch Of Microorganisms That Produce N-6 Fatty Acmentioning

confidence: 99%

“…This regulation of PUFA metabolic flux is partly due to the substrate specificity of the ∆6 Des Table 2, Sakuradani and Shimizu, 2003;Sakuradani et al, 2005;Zhu et al, 2002) which prefers n-6 fatty acid LA to n-3 ALA as substrate and its ω-3 Des must be repressed at physiological growth temperature (Shimizu et al, 1998). Although the ω-3 Des have been cloned and biochemically studied in a few microorganisms Table 5, (Gellerman and Schlenk, 1979;Pereira et al, 2004;Sakamoto et al, 1994;Wada and Murata, 1990), its expression level in oleaginous microorganisms has not been determined so far. However over-expression of ω-3 Des in Mt.…”

Section: Molecular Switch Of Microorganisms That Produce N-6 Fatty Acmentioning

confidence: 99%

“…PCC 6803 was expressed in Anabaena, substrate preference was calculated based on its endogenous fatty acids production. ∆6 Des from all other organisms were expressed in S. cerevisiae and the substrate preference was studied by feeding the yeast with exogenous precursor fatty acids as substrates (Wada and Murata, 1990;SDA: 8 <1 25 ? Sakamoto et al, 1994) *Strain was cultivated at 28°C †Strain was cultivated at 12°C §Yield of substrate conversion was calculated as described previously (Sakuradani et al, 2005), Conversion yield (%) = 100× ([product]/ [product + substrate].…”

Section: Ajbbmentioning

confidence: 99%

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Molecular Switch That Controls the Flux of Linoleic Acid Into N-6 or N-3 Polyunsaturated Fatty Acids in Microorganisms

Wang

Chen²,

Zhang

et al. 2014

American Journal of Biochemistry and Biotechnology

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Polyunsaturated Fatty Acids (PUFA) of the n-6 and n-3 series play important roles in nutrition. Microorganisms are important sources of n-6 and n-3 fatty acids; however, most produce either n-6 or n-3 fatty acids as the major PUFAs and very few produce both. This differential production suggests that PUFAs metabolic pathway is strictly controlled in microorganisms. The major pathway of n-6/n-3 faty acids biosynthesis in lower eukaryotes is composed of ∆12 Desaturase (Des), ω3 Des (∆15, ∆17), ∆6 Des, ∆6 Elongase (Elo), ∆5 Des, ∆5 Elo and ∆4 Des, among which ∆6 Des and ∆15 (ω3) Des, located at the branch point of PUFAs metabolic pathways, are key regulators of the flux of linoleic acid (18:2 n-6) into either n-6 or n-3 fatty acid metabolic pathways. These latter two enzymes work together as a molecular switch that control the production of n-6/n-3 fatty acids. However the mechanism of the molecular switch is, so far, not clear. This review summarizes the recent advancement of the molecular base of the differentail production of n-6 or n-3 PUFAs in microorganisms.

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Temperature-Induced Changes in the Fatty Acid Composition of the Cyanobacterium, Synechocystis PCC6803

Cited by 175 publications

References 29 publications

Connecting thermal physiology and latitudinal niche partitioning in marine Synechococcus

Connecting thermal physiology and latitudinal niche partitioning in marine Synechococcus

Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses

Molecular Switch That Controls the Flux of Linoleic Acid Into N-6 or N-3 Polyunsaturated Fatty Acids in Microorganisms

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