Temperature induction response (TIR) as a rapid screening protocol to dissect the genetic variability in acquired thermotolerance in rice and to identify novel donors for high temperature stress tolerance

Vijayalakshmi, D.; Srividhya, S.; Vivitha, P.; Raveendran, M.

doi:10.1007/s40502-015-0192-1

Cited by 14 publications

(16 citation statements)

References 21 publications

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“…By contrast, significant differences were observed between the survival rates of the varieties that underwent the TIR50 treatment. After undergoing the TIR treatment, the rice seedlings’ thermotolerance was substantially improved; this finding concurred with that of Vijayalakshmi et al (2015). Harihar et al (2014) and Vijayalakshmi et al (2015) employed growth and survival rates to screen plants following TIR treatment.…”

Section: Discussionsupporting

confidence: 90%

“…After undergoing the TIR treatment, the rice seedlings’ thermotolerance was substantially improved; this finding concurred with that of Vijayalakshmi et al (2015). Harihar et al (2014) and Vijayalakshmi et al (2015) employed growth and survival rates to screen plants following TIR treatment. However, the results of the present study indicated that the survival and growth rates were slightly correlated.…”

Section: Discussionsupporting

confidence: 90%

“…TIR can be used to evaluate short-term acquired thermotolerance. The treatment method in this study referenced that in Harihar et al (2014) and Vijayalakshmi et al (2015) and was aimed at providing solutions to the high air- and water-temperature stress faced by seedlings during Taiwan’s second crop seedling period. The study results indicated that the varieties subjected to the TIR48 treatment demonstrated significant differences in the shoot RGR.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the seedlings were subjected to extremely high temperature treatments to compare the thermotolerance performance. The TIR technique has been applied to screen thermotolerant peas (Srikanthbabu et al 2002), cotton (Kheir et al 2012), Eleusine coracana (Babu et al 2013), and rice (Harihar et al 2014; Vijayalakshmi et al 2015), thereby demonstrating the TIR technique to be an effective screening method for acquiring thermotolerant varieties.…”

Section: Introductionmentioning

confidence: 99%

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Thermotolerance evaluation of Taiwan Japonica type rice cultivars at the seedling stage

Hsuan

Jhuang

et al. 2019

Bot Stud

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BackgroundThe subtropical rice varieties grown in Taiwan are mainly Japonica-type rice varieties, which are grown in the southernmost- and lowest-latitude Japonica type rice cultivation area in the world. In Taiwan, seedlings that are planted either by transplanting or direct seeding in the second crop will face the season with the highest temperatures during the year. High-temperature stress severely influences early rice growth and causes yield losses. With global warming deteriorating, this problem is becoming increasingly severe. This study attempted to establish a high-efficiency and time-saving screening tool for rice varieties that exhibit thermotolerance during the early growth stages and further identify good donors with better tolerance for high temperature stress from Taiwan Japonica type rice germplasm.ResultsDuring the initial germination stage, there were significantly different responses to heat stress among the different rice varieties. After the temperature induction response technique (TIR) treatment, the seedling survival rate and relative growth rate of the rice varieties under high temperature stress were significantly improved. In addition, the seedlings of the thermotolerant varieties demonstrated greater thermotolerance performance in the pot experiment as well as cell membrane stability (CMS) and cell activity (2,3,5-triphenyl-tetrazolium chloride; TTC) test results. However, the correlation between the thermotolerance of the seedlings and seeds was low. A phylogenetic dendrogram was plotted and revealed that thermotolerant genes did not concentrate in specific clusters. Furthermore, there was a non-significant correlation between the thermotolerance of the varieties and the years in which they were released.ConclusionsThe temperature induction screening tool established by this study could determine the potential of each variety to adapt to high temperature stress. Additionally, thermotolerance during different growth stages (i.e., the germination, seedling, and grain maturation stages) exhibited low correlations. In this study, the varieties obtained through preliminary screening (i.e., TK14, HC56, TT30, TNG70, and TK8) exhibited outstanding thermotolerance. The screen tools and thermotolerance varieties could be valuable resources for the countries that grow Japonica type rice to apply when breeding thermotolerant varieties in the future.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thermotolerance evaluation of Taiwan Japonica type rice cultivars at the seedling stage

Hsuan

Jhuang

et al. 2019

Bot Stud

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“…The technique of exposing young seedlings to sub lethal and lethal temperatures has been validated in many crop species Venkatachalayya et al (2001) in pea , Senthil-kumar et al (2003) in sunflower, Gangappa et al (2006) in groundnut, Sudhakar et al ( 2012 ), Renukha et al (2013) and Vijayalakshmi et al (2015) in rice, Ehab abou kheir et al (2012) in cotton, This novel temperature induction response technique has been demonstrated to reveal genetic vaiability in intrinsic stress tolerant at cellular level, Narayanaswamy (2010).…”

Section: Resultsmentioning

confidence: 99%

Temperature Induction Response (TIR) - A novel physiological approach for thermotolerant genotypes in chickpea (Cicer arietinum L.)

Raghavendra

Jayalakshmi

Babu

2017

IJARe

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<span>Chickpea (Cicer arietinum L.) is an important food legume cultivated in arid and semi-arid regions of the world. It is known that chickpea thrives well under drought prone condition. However, heat stress during reproductive development can cause significant yield loss and there is a greater variability for yield performance of chickpea under heat stress. It is extremely important to develop screening tools for identification of thermotolerant chickpea genotypes in view of increase in average global temperatures. In this context, a lab experiment was conducted to standardize the temperature induction response (TIR) protocol for chickpea seedlings using WGC-450 programmable plant growth chamber. Temperatures were standardized as sub lethal i.e. challenging temperatures as 38-480C (for 4 hours &30 min) and lethal temperatures as 500C (for 3 hours). This technique can be used as a potential tool to identify and select temperature tolerant lines at the seedling stage from a large population. A set of diverse chickpea germplasm comprising of 57 genotypes were screened for intrinsic tolerance using the standardized Thermo Induced Response (TIR) protocol. Among the genotypes NBeG-528,NBeG-458,NBeG-511,NBeG-177,NBeG-747,NBeG-732 and VIHAR showed highest thermo tolerance in terms of higher seedling survival with no reduction in root and shoot growth. The genotypes with intrinsic heat tolerance can be explored for the development of varieties suitable for late sown conditions in Andhra Pradesh where chickpea is prone to terminal heat stress.</span>

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Identification of differentially expressed genes under heat stress conditions in rice (Oryza sativa L.)

et al. 2020

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Temperature induction response (TIR) as a rapid screening protocol to dissect the genetic variability in acquired thermotolerance in rice and to identify novel donors for high temperature stress tolerance

Cited by 14 publications

References 21 publications

Thermotolerance evaluation of Taiwan Japonica type rice cultivars at the seedling stage

Thermotolerance evaluation of Taiwan Japonica type rice cultivars at the seedling stage

Temperature Induction Response (TIR) - A novel physiological approach for thermotolerant genotypes in chickpea (Cicer arietinum L.)

Identification of differentially expressed genes under heat stress conditions in rice (Oryza sativa L.)

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