Temperature modulates virus‐induced transcriptional gene silencing via secondary small RNAs

Fei, Yue; Pyott, Douglas E.; Molnár, Attila

doi:10.1111/nph.17586

Cited by 20 publications

(15 citation statements)

References 71 publications

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“…TaWRKY74 and TaCRR6 RNAi fragments were cloned into BSMV‐γ vectors for VIGS assays in T. aestivum (Yang et al., 2018) . NbWRKY17 and NbCRR6 RNAi fragments were cloned into TRV2 for VIGS in N. benthamiana (Fei et al., 2021). The primers and restriction enzymes used are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

The ‘Candidatus Phytoplasma tritici’ effector SWP12 degrades the transcription factor TaWRKY74 to suppress wheat resistance

Bai

Zhang

et al. 2022

The Plant Journal

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SUMMARY ‘Candidatus Phytoplasma tritici’ (‘Ca. P. tritici’) is an insect‐borne obligate pathogen that infects wheat (Triticum aestivum) causing wheat blue dwarf disease, and leads to yield losses. SWP12 is a potential effector secreted by ‘Ca. P. tritici’ that manipulates host processes to create an environment conducive to phytoplasma colonization, but the detailed mechanism of action remains to be investigated. In this study, the expression of SWP12 weakened the basal immunity of Nicotiana benthamiana and promoted leaf colonization by Phytophthora parasitica, Sclerotinia sclerotiorum, and tobacco mild green mosaic virus. Moreover, the expression of SWP12 in wheat plants promoted phytoplasma colonization. Triticum aestivum WRKY74 and N. benthamiana WRKY17 were identified as host targets of SWP12. The expression of TaWRKY74 triggered reactive oxygen species bursts, upregulated defense‐related genes, and decreased TaCRR6 transcription, leading to reductions in NADH dehydrogenase complex (NDH) activity. Expression of TaWRKY74 in wheat increased plant resistance to ‘Ca. P. tritici’, and silencing of TaWRKY74 enhanced plant susceptibility, which indicates that TaWRKY74 is a positive regulator of wheat resistance to ‘Ca. P. tritici’. We showed that SWP12 weakens plant resistance and promotes ‘Ca. P. tritici’ colonization by destabilizing TaWRKY74.

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Section: Methodsmentioning

confidence: 99%

The ‘Candidatus Phytoplasma tritici’ effector SWP12 degrades the transcription factor TaWRKY74 to suppress wheat resistance

Bai

Zhang

et al. 2022

The Plant Journal

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“…Lastly, the timing of the VIGS phenotype appearance and the duration for which the gene silencing effect lasts is speciesspecific. Also, an effect of the ambient temperature on VIGS through secondary siRNA production was reported (Fei, Pyott and Molnar, 2021).…”

Section: Dna and Rna Virus Modifications For Virus-induced Gene Silen...mentioning

confidence: 99%

Plant biomacromolecule delivery methods in the 21st century

et al. 2022

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The 21st century witnessed a boom in plant genomics and gene characterization studies through RNA interference and site-directed mutagenesis. Specifically, the last 15 years marked a rapid increase in discovering and implementing different genome editing techniques. Methods to deliver gene editing reagents have also attempted to keep pace with the discovery and implementation of gene editing tools in plants. As a result, various transient/stable, quick/lengthy, expensive (requiring specialized equipment)/inexpensive, and versatile/specific (species, developmental stage, or tissue) methods were developed. A brief account of these methods with emphasis on recent developments is provided in this review article. Additionally, the strengths and limitations of each method are listed to allow the reader to select the most appropriate method for their specific studies. Finally, a perspective for future developments and needs in this research area is presented.

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“…As for sNRT2.3-1, the temperature shifts from 25° to 32°C changed the hairpin loop sequences from UGUU-GGAGAUGGAG into GAUGGA (Fig. 5, A and D), which would affect the binding to the target mRNA (14,15).…”

Section: Srnas Promote the Adaptation Of Rice To High Temperature By ...mentioning

confidence: 99%

High-temperature adaptation of an OsNRT2.3 allele is thermoregulated by small RNAs

et al. 2022

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Climate change negatively affects crop yield, which hinders efforts to reach agricultural sustainability and food security. Here, we show that a previously unidentified allele of the nitrate transporter gene OsNRT2.3 is required to maintain high yield and high nitrogen use efficiency under high temperatures. We demonstrate that this tolerance to high temperatures in rice accessions harboring the HTNE-2 (high temperature resistant and nitrogen efficient-2) alleles from enhanced translation of the OsNRT2.3b mRNA isoform and the decreased abundance of a unique small RNA (sNRT2.3-1) derived from the 5′ untranslated region of OsNRT2.3 . sNRT2.3-1 binds to the OsNRT2.3a mRNA in a temperature-dependent manner. Our findings reveal that allelic variation in the 5′ untranslated region of OsNRT2.3 leads to an increase in OsNRT2.3b protein levels and higher yield during high-temperature stress. Our results also provide a breeding strategy to produce rice varieties with higher grain yield and lower N fertilizer input suitable for a sustainable agriculture that is resilient against climate change.

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Temperature modulates virus‐induced transcriptional gene silencing via secondary small RNAs

Cited by 20 publications

References 71 publications

The ‘Candidatus Phytoplasma tritici’ effector SWP12 degrades the transcription factor TaWRKY74 to suppress wheat resistance

The ‘Candidatus Phytoplasma tritici’ effector SWP12 degrades the transcription factor TaWRKY74 to suppress wheat resistance

Plant biomacromolecule delivery methods in the 21st century

High-temperature adaptation of an OsNRT2.3 allele is thermoregulated by small RNAs

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