Temperature profiled simultaneous saccharification and co-fermentation of corn stover increases ethanol production at high solid loading

Zhu, Jiaqing; Zong, Qiu-Jin; Li, Wenchao; Chai, Meng-Zhe; Xu, Tao; Liu, Hong; Fan, Hong-Tao; Li, Bingzhi; Yuan, Ying‐Jin

doi:10.1016/j.enconman.2019.112344

Cited by 34 publications

(7 citation statements)

References 46 publications

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“…Corn stover is an agricultural residue whose suitability and great potential to obtain bioethanol has been extensively evaluated [13][14][15]. For that, a pretreatment that allows an optimal fractionation of the raw material is essential.…”

Section: Introductionmentioning

confidence: 99%

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Río¹,

Gullón²,

Rebelo³

et al. 2020

Agronomy

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Corn stover is the most produced byproduct from maize worldwide. Since it is generated as a residue from maize harvesting, it is an inexpensive and interesting crop residue to be used as a feedstock. An ecologically friendly pretreatment such as autohydrolysis was selected for the manufacture of second-generation bioethanol from corn stover via whole-slurry fermentation at high-solid loadings. Temperatures from 200 to 240 °C were set for the autohydrolysis process, and the solid and liquid phases were analyzed. Additionally, the enzymatic susceptibility of the solid phases was assessed to test the suitability of the pretreatment. Afterward, the production of bioethanol from autohydrolyzed corn stover was carried out, mixing the solid with different percentages of the autohydrolysis liquor (25%, 50%, 75%, and 100%) and water (0% of liquor), from a total whole slurry fermentation (saving energy and water in the liquid–solid separation and subsequent washing of the solid phase) to employing water as only liquid medium. In spite of the challenging scenario of using the liquor fraction as liquid phase in the fermentation, values between 32.2 and 41.9 g ethanol/L and ethanol conversions up to 80% were achieved. This work exhibits the feasibility of corn stover for the production of bioethanol via a whole-slurry fermentation process.

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Section: Introductionmentioning

confidence: 99%

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Río¹,

Gullón²,

Rebelo³

et al. 2020

Agronomy

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“…Co-fermentation with yeast tolerating inhibitors and yeast utilizing xylose was conducted in the SScF process in undetoxified pretreated biomass to overcome the problem of xylose utilization and inhibitor problems [ 60 ]. Co-culture with high-temperature resistant and xylose-utilizing S. cerevisiae was developed for ethanol production in temperature-profiled SScF processes [ 61 ]. Previous studies indicated that the synergy of high temperature and inhibitors resulted in low viability of the strains in the SScF process [ 58 ], and the strain BY4742/IrrE and the mutant strains developed in this study will be helpful for the bioethanol production from SScF of lignocellulose at high temperatures.…”

Section: Resultsmentioning

confidence: 99%

Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast

Wang

et al. 2020

Biotechnol Biofuels

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Background Stress tolerance is one of the important desired microbial traits for industrial bioprocesses, and global regulatory protein engineering is an efficient approach to improve strain tolerance. In our study, IrrE, a global regulatory protein from the prokaryotic organism Deinococcus radiodurans, was engineered to confer yeast improved tolerance to the inhibitors in lignocellulose hydrolysates or high temperatures. Results Three IrrE mutations were developed through directed evolution, and the expression of these mutants could improve the yeast fermentation rate by threefold or more in the presence of multiple inhibitors. Subsequently, the tolerance to multiple inhibitors of single-site mutants based on the mutations from the variants were then evaluated, and 11 mutants, including L65P, I103T, E119V, L160F, P162S, M169V, V204A, R244G, Base 824 Deletion, V299A, and A300V were identified to be critical for the improved representative inhibitors, i.e., furfural, acetic acid and phenol (FAP) tolerance. Further studies indicated that IrrE caused genome-wide transcriptional perturbation in yeast, and the mutant I24 led to the rapid growth of Saccharomyces cerevisiae by primarily regulating the transcription level of transcription activators/factors, protecting the intracellular environment and enhancing the antioxidant capacity under inhibitor environments, which reflected IrrE plasticity. Meanwhile, we observed that the expression of the wild-type or mutant IrrE could also protect Saccharomyces cerevisiae from the damage caused by thermal stress. The recombinant yeast strains were able to grow with glucose at 42 ℃. Conclusions IrrE from Deinococcus radiodurans can be engineered as a tolerance-enhancer for Saccharomyces cerevisiae. Systematic research on the regulatory model and mechanism of a prokaryotic global regulatory factor IrrE to increase yeast tolerance provided valuable insights for the improvements in microbial tolerance to complex industrial stress conditions.

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“…Use of eukaryotic systems like KluveromycesmarxianusNCYC 587, Mucor indicus, dry active yeast, S. cerevisiae 424A, S. cerevisiae D5A, S. cerevisiae SyBE005, co-culturing temperature resistant S. cerevisiae and xylose utilising S. cerevisiae and prokaryotic Bacillus coagulans CC17 for attaining high product titres are few examples that reaffirm that use of temperature resistant, product tolerant and multiple sugar assimilating microbes for high solids hydrolysis and fermentation is booming [26,28,30,43,45,53,57,58,79].…”

Section: Upcoming Trends In Fermentationmentioning

confidence: 99%

Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

Baral

Kumar

Agrawal

2021

Critical Reviews in Biotechnology

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For the techno-commercial success of any lignocellulosic biorefinery, the cost-effective production of fermentable sugars for the manufacturing of bio-based products is indispensable. High-solids enzymatic saccharification (HSES) is a straightforward approach to developing an industrially deployable sugar platform.Economic incentives such as reduced capital and operational expenditure along with environmental benefits in the form of reduced effluent discharge make this strategy more lucrative for exploitation. However, HSES suffers from the drawback of non-linear and disproportionate sugar yields with increased substrate loadings. To overcome this bottleneck, researchers tend to perform HSES at high enzyme loadings. Nonetheless, the production cost of cellulases is one of the key contributors that impair the entire process economics. This review highlights the relentless efforts made globally to attain a high-titre of sugars and their fermentation products by performing efficient HSES at low cellulase loadings. In this context, technical innovations such as advancements in new pretreatment strategies, next-generation cellulase cocktails, additives, accessory enzymes, novel reactor concepts and enzyme recycling studies are especially showcased. The review further covers new insights, learnings and prospects in the area of lignocellulosic bioprocessing.

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Temperature profiled simultaneous saccharification and co-fermentation of corn stover increases ethanol production at high solid loading

Cited by 34 publications

References 46 publications

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast

Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

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