Temperature responsive lipid liquid crystal layers with embedded nanogels

Dabkowska, Aleksandra P.; Hirst, Christopher; Valldeperas, Maria; Clifton, Luke A.; Montis, Costanza; Nojd, Söfi; Gentile, Luigi; Wang, M.; Pálsson, Gunnar K.; Lages, Sebastian; Berti, Debora; Barauskas, Justas; Nylander, Tommy

doi:10.1039/c6cc09426k

Cited by 13 publications

(15 citation statements)

References 17 publications

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“…Lipid mixtures with and without PNIPAM nanogel in ethanol were investigated on the Ganesha 300XL (SAXSLAB ApS, Skovlunde, Denmark). The instrument has a Genix 3D X-ray source, with a l ¼ 1.54 Å 21 and a two-dimensional 300 K Pilatus detector from Dectris. Samples were exposed for 45 min at seven different temperatures (20, 25, 27.5, 30, 32.5, 35 and 408C) controlled by an external recirculating water bath.…”

Section: Small Angle X-ray Scatteringmentioning

confidence: 99%

Non-lamellar lipid assembly at interfaces: controlling layer structure by responsive nanogel particles

et al. 2017

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Biological membranes do not only occur as planar bilayer structures, but depending on the lipid composition, can also curve into intriguing three-dimensional structures. In order to fully understand the biological implications as well as to reveal the full potential for applications, e.g. for drug delivery and other biomedical devices, of such structures, well-defined model systems are required. Here, we discuss the formation of lipid non-lamellar liquid crystalline (LC) surface layers spin-coated from the constituting lipids followed by hydration of the lipid layer. We demonstrate that hybrid lipid polymer films can be formed with different properties compared with the neat lipid LC layers. The nanostructure and morphologies of the lipid films formed reflect those in the bulk. Most notably, mixed lipid layers, which are composed of glycerol monooleate and diglycerol monooleate with poly(-isopropylacrylamide) nanogels, can form films of reverse cubic phases that are capable of responding to temperature stimulus. Owing to the presence of the nanogel particles, changing the temperature not only regulates the hydration of the cubic phase lipid films, but also the lateral organization of the lipid domains within the lipid self-assembled film. This opens up the possibility for new nanostructured materials based on lipid-polymer responsive layers.

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Section: Small Angle X-ray Scatteringmentioning

confidence: 99%

Non-lamellar lipid assembly at interfaces: controlling layer structure by responsive nanogel particles

et al. 2017

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“…In this framework, lipid models of synthetic nature, mimicking cubic membranes' structure, can be used to simplify the investigation. Our recent findings [22][23][24] show that it is possible to obtain solid-supported lipid model surface layers of cubic symmetry, with controlled physicochemical and structural features, enabling the study of NPs-cubic membranes in highly simplified and controlled conditions.…”

Section: Introductionmentioning

confidence: 98%

Interaction of nanoparticles with lipid films: the role of symmetry

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Ridolfi

Mangiapia

et al. 2021

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When nanomaterials are put in contact with living organisms, their interaction with biomacromolacules and biological barriers will determine their bioactivity, biological fate and cytotoxicity. In this context, the role of symmetry/shape anisotropy of both the nanomaterials and the biological interfaces on their interaction mechanism, is a relatively unaddressed issue. Here, we study the interaction of gold nanoparticles (NPs) of different shape (nanospheres and nanorods) with biomimetic membranes of different symmetry, i.e. flat membranes (of 2D symmetry, representative of the most common plasma membrane geometry), and cubic membranes (of 3D symmetry, representative of non-lamellar membranes, encountered in Nature in peculiar biological conditions). Through an ensemble of structural techniques (Neutron Reflectometry, Grazing Incidence Small-Angle Neutron Scattering), we found that, on a nanometric lengthscale, the structural stability of the membrane towards NPs is dependent on the topological characteristic of the lipid assembly and of the NPs, with higher symmetry related to higher stability. In addition, Confocal Laser Scanning Microscopy analyses highlight, on a micrometric legthscale, that cubic and lamellar phases interact with NPs according to two distinct mechanisms, related to the different structures of the lipid assemblies. This study represents a first attempt to systematically study the role of membrane symmetry on the interaction with NPs; the results will contribute to improve the fundamental knowledge on nano-bio interfaces and, more in general, will provide new insights on the biological function of non-lamellar cubic arrangements of interfacial membranes as a response strategy.

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“…cubosomes, mostly for drug delivery purposes [9][10][11] . On the other hand, fewer works in literature focused on the development of homogeneous supported lipid QII phase films [12][13][14][15] .Similarly to Supported Lipid Bilayers (SLBs) 16 , these membrane models could be exploited for characterizing the physicochemical and mechanical properties of natural lipid QII membranes and applied as biosensing platforms in interface interaction studies. .…”

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“…Our analysis of the PtP distance distribution also provides the first example in the literature on the use of AFM-FS for characterizing the structure of non-lamellar lipid membranes. Despite lacking the accuracy of the more traditional scattering techniques (involving both X-rays and/or neutrons) 12,21,22 and cryo-electron microscopy (cryo-EM) 5,27 , in this context, AFM-FS represents a more accessible and still reliable solution for obtaining a quick structural analysis of these lipid mesophases.…”

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