Temperature, season, and latitude influence development-related phenotypes of Philippine Aedes aegypti (Linnaeus): Implications for dengue control amidst global warming

Edillo, Frances E.; Ymbong, Rhoniel Ryan; Bolneo, Alyssa Angel; Hernandez, Ric Jacob; Fuentes, Bianca Louise; Cortes, Garren; Cabrera, J G; Lazaro, J. Enrico H.; Sakuntabhai, Anavaj

doi:10.1186/s13071-022-05186-x

Cited by 12 publications

(16 citation statements)

References 66 publications

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“…The Philippines continues to experience the health burden caused by high dengue cases, ranking number one in Asia with 17,630 deaths last March 2021 ( Edillo et al., 2015 , 2022 ; Ong et al., 2022 ). The persistent high cases annually prompted the Philippine government to establish the National Dengue Prevention and Control Program with vector surveillance and management as one of the key targets ( Dengue Prevention and Control Program | Department of Health website, n.d. ).…”

Section: Introductionmentioning

confidence: 99%

“…The persistent high cases annually prompted the Philippine government to establish the National Dengue Prevention and Control Program with vector surveillance and management as one of the key targets ( Dengue Prevention and Control Program | Department of Health website, n.d. ). Aedes aegypti mosquito is the primary vector of Dengue in the Philippines owing to its adaptability in changing environments ( Edillo et al., 2022 ). To mitigate infections, recommendations for vector management including the reduction of breeding sites, and improvement of water systems have been proposed.…”

Section: Introductionmentioning

confidence: 99%

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Detection and quantification of natural Wolbachia in Aedes aegypti in Metropolitan Manila, Philippines using locally designed primers

Reyes,

Suzuki,

Suzuki

et al. 2024

Front. Cell. Infect. Microbiol.

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BackgroundThe Philippines bears health and economic burden caused by high dengue cases annually. Presently, the Philippines still lack an effective and sustainable vector management. The use of Wolbachia, a maternally transmitted bacterium, that mitigate arbovirus transmission has been recommended. Cytoplasmic incompatibility and viral blocking, two characteristics that make Wolbachia suitable for vector control, depend on infection prevalence and density. There are no current Wolbachia release programs in the Philippines, and studies regarding the safety of this intervention. Here, we screened for Wolbachia in Aedes aegypti collected from Metropolitan Manila, Philippines. We designed location-specific primers for qPCR to test whether this improved Wolbachia detection in Ae. aegypti. We explored if host sex and Wolbachia strain could be potential factors affecting Wolbachia density.MethodsAe. aegypti mosquitoes (n=429) were screened for natural Wolbachia by taqman qPCR using location-specific Wolbachia surface protein primers (wspAAML) and known 16S rRNA primers. Samples positive for wspAAML (n=267) were processed for Sanger sequencing. We constructed a phylogenetic tree using IQ-TREE 2 to further characterize Wolbachia present in the Philippine Ae. aegypti. We then compared Wolbachia densities between Wolbachia groups and host sex. Statistical analyses were done using GraphPad Prism 9.0.ResultsWolbachia prevalence for 16S rRNA (40%) and wspAAML (62%) markers were high. Wolbachia relative densities for 16S rRNA ranged from −3.84 to 2.71 and wspAAML from −4.02 to 1.81. Densities were higher in male than female mosquitoes. Wolbachia strains detected in Ae. aegypti clustered into supergroup B. Some 54% (123/226) of these sequences clustered under a group referred to here as “wAegML,” that belongs to the supergroup B, which had a significantly lower density than wAegB/wAlbB, and wAlbA strains.ConclusionLocation-specific primers improved detection of natural Wolbachia in Ae. aegypti and allowed for relative quantification. Wolbachia density is relatively low, and differed between host sexes and Wolbachia strains. An economical way of confirming sporadic or transient Wolbachia in Ae. aegypti is necessary while considering host sex and bacterial strain.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection and quantification of natural Wolbachia in Aedes aegypti in Metropolitan Manila, Philippines using locally designed primers

Reyes,

Suzuki,

Suzuki

et al. 2024

Front. Cell. Infect. Microbiol.

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“…ROs were highest at 25 degrees Celsius in the wet season and at 18 degrees Celsius in the dry season. Furthermore, the study noted that the incidence of the dengue-causing mosquito phenotype was found to be highest at 18 degrees Celsius and present even at 38 degrees Celsius in both seasons, suggesting larval adaptation to global warming [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Socio-economic factors and its influence on the association between temperature and dengue incidence in 61 Provinces of the Philippines, 2010–2019

Seposo,

Valenzuela,

Apostol

2023

PLoS Negl Trop Dis

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Background Temperature has a significant impact on dengue incidence, however, changes on the temperature-dengue relationship across axes of socio-economic vulnerability is not well described. This study sought to determine the association between dengue and temperature in multiple locations in the Philippines and explore the effect modification by socio-economic factors. Method Nationwide dengue cases per province from 2010 to 2019 and data on temperature were obtained from the Philippines’ Department of Health–Epidemiological Bureau and ERA5-land, respectively. A generalized additive mixed model (GAMM) with a distributed lag non-linear model was utilized to examine the association between temperature and dengue incidence. We further implemented an interaction analysis in determining how socio-economic factors modify the association. All analyses were implemented using R programming. Results Nationwide temperature-dengue risk function was noted to depict an inverted U-shaped pattern. Dengue risk increased linearly alongside increasing mean temperature from 15.8 degrees Celsius and peaking at 27.5 degrees Celsius before declining. However, province-specific analyses revealed significant heterogeneity. Socio-economic factors had varying impact on the temperature-dengue association. Provinces with high population density, less people in urban areas with larger household size, high poverty incidence, higher health spending per capita, and in lower latitudes were noted to exhibit statistically higher dengue risk compared to their counterparts at the upper temperature range. Conclusions This observational study found that temperature was associated with dengue incidence, and that this association is more apparent in locations with high population density, less people in urban areas with larger household size, high poverty incidence, higher health spending per capita, and in lower latitudes. Differences with socio-economic conditions is linked with dengue risk. This highlights the need to develop interventions tailor-fit to local conditions.

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“…The incidence of dengue in the Philippines increased from 37,101 in 2006 to 118,868 in 2010 [ 10 ]. Significant outbreaks since 2012 recorded 187,000 dengue cases and as many as 270,00 cases between January and August 2019 [ 11 ], ranking the country fourth out of ten in ASEAN [ 12 ]. The degradation of the environment due to urbanization, an increase in population, poor waste disposal strategies, changes in weather conditions, and poor surveillance of mosquito breeding sites are among the factors that contribute to the rise in dengue cases in the country [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Efficacy Assessment of Autodissemination Using Pyriproxyfen-Treated Ovitraps in the Reduction of Dengue Incidence in Parañaque City, Philippines: A Spatial Analysis

et al. 2023

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Dengue is one of the most important vector-borne diseases worldwide and is a significant public health problem in the tropics. Mosquito control continues to be the primary approach to reducing the disease burden and spread of dengue virus (DENV). Aside from the traditional larviciding and adulticiding interventions, autodissemination using pyriproxyfen-treated (AD-PPF) ovitraps is one of the promising methods to complement existing vector control strategies. Our paper assessed the efficacy of AD-PPF in reducing DENV infections in two barangays in Parañaque City. Using saliva samples from the participants from both the control and intervention sites, we collected the seroprevalence data for three months in each of the two years. Spatial analysis was conducted to determine hotspot areas and identify DENV infection distributions across the trial periods. The results showed that the intervention site was identified as having a clustering of DENV infections in Month 0 of Year 1 and shifted to a random dispersion of dengue cases at the end of Month 3 in Year 2. The disappearance of the clustering of the intervention site translates to a decrease in the cases of DENV infection relative to the control site. Furthermore, we also identified that DENV transmission occurred at a small-scale level that did not go beyond 86 m. In conclusion, AD-PPF is suggested to be an effective strategy and may be used as an additional vector control approach, albeit based on this short-term implementation.

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Temperature, season, and latitude influence development-related phenotypes of Philippine Aedes aegypti (Linnaeus): Implications for dengue control amidst global warming

Cited by 12 publications

References 66 publications

Detection and quantification of natural Wolbachia in Aedes aegypti in Metropolitan Manila, Philippines using locally designed primers

Detection and quantification of natural Wolbachia in Aedes aegypti in Metropolitan Manila, Philippines using locally designed primers

Socio-economic factors and its influence on the association between temperature and dengue incidence in 61 Provinces of the Philippines, 2010–2019

Efficacy Assessment of Autodissemination Using Pyriproxyfen-Treated Ovitraps in the Reduction of Dengue Incidence in Parañaque City, Philippines: A Spatial Analysis

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