Temperature Sensitivity of Wild-Type, Mutant and Genetic Sexing Strains of Ceratitis capitata

Sollazzo, Germano; Gouvi, Georgia; Nikolouli, Katerina; Martinez, Elena I. Cancio Cancio; Schetelig, Marc F.; Bourtzis, Kostas

doi:10.3390/insects13100943

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…All four strains were kept under standard laboratory conditions at 24 ± 2°C, 55 ± 10% RH, and 14/10 h light/dark cycle. Adults were fed on yeast and sugar (1:3) with water being provided separately, while larvae were reared on a carrot diet as described previously ( Sollazzo et al . 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region

Sollazzo

Gouvi

Nikolouli

et al. 2023

G3: Genes, Genomes, Genetics

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Genetic sexing strains (GSS) are an important tool in support of sterile insect technique (SIT) applications against insect pests and disease vectors. The yet unknown temperature-sensitive lethal (tsl) gene and the recently identified white pupae (wp) gene have been used as selectable markers in the most successful GSS developed so far, the Ceratitis capitata (medfly) VIENNA 8 GSS. The molecular identification of the tsl gene may open the way for its use as a marker for the development of GSS in other insect pests and disease vectors of SIT importance. Prior studies have already shown that the tsl gene is located on the right arm of chromosome 5, between the wp and Zw loci (tsl genomic region). In the present study, we used genomic, transcriptomic, bioinformatic, and cytogenetic approaches to characterize and analyze this genomic region in wild-type and tsl mutant medfly strains. Our results suggested the presence of 561 genes, with 322 of them carrying SNPs and/or insertion-deletion (indel) mutations in the tsl genomic region. Furthermore, comparative transcriptomic analysis indicated the presence of 32 differentially expressed genes, and bioinformatic analysis revealed the presence of 33 orthologs with a described heat-sensitive phenotype of Drosophila melanogaster in this region. These data can be used in functional genetic studies to identify the tsl gene(s) and the causal mutation(s) responsible for the temperature-sensitive lethal phenotype in medfly, and potentially additional genes causing a similar phenotype.

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Section: Methodsmentioning

confidence: 99%

Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region

Sollazzo

Gouvi

Nikolouli

et al. 2023

G3: Genes, Genomes, Genetics

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“…Since the global surface annual temperature has increased at an average rate of 0.1°C, almost double compared to 20 years ago, and increases of 1.5°C and 2-4°C are expected by 2050 and 2100, respectively [69], rising temperatures can severely affect an AW-IPM program because temperature changes can influence the longevity, flight ability, and mating performance of sterile males. An elevated temperature could lead to the death of sterile males released during SIT [70]. Numerous studies have recently suggested that the gut microbiota is sensitive to environmental temperature, which induces changes in its composition and diversity, and may have significant consequences on host phenotype and fitness [71][72][73].…”

Section: Temperature Tolerancementioning

confidence: 99%

Probiotics as a Beneficial Modulator of Gut Microbiota and Environmental Stress for Sustainable Mass-Reared Ceratitis capitata

Guerfali¹,

Hamden²,

Charaabi³

et al. 2024

Advances in Probiotics for Health and Nutrition

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The Mediterranean fruit fly Ceratitis capitata (medfly) is a major pest throughout the world and one of the most destructive. Several strategies for controlling this pest have been proposed, including the sterile insect technique (SIT). The SIT’s effectiveness against the medfly is well documented. Sterile medflies, on the other hand, can perform poorly. Reduced mating compatibility and mating competitiveness in the field may be caused by genetic and symbiotic differences between natural and laboratory medfly populations. Probiotic gut symbionts have been shown to facilitate control strategies and improve male medfly fitness. They are equally effective in the live and inactivated forms when administered to medfly adults or larvae. They have been shown to modulate a large set of inducible effector molecules including antimicrobial peptides (AMP) and stress-responsive proteins. The selection procedures of probiotics for their use in the medfly rearing process are reviewed, and other pathways for selection are proposed based on recent in silico studies. This chapter summarizes the most relevant evidence from scientific literature regarding potential applications of probiotics in medfly as an innovative tool for biocontrol, while also shedding light on the spectrum of symbiotic relationships in medfly that may serve as a powerful symbiotic integrative control approach.

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“…In China, researchers have developed a genetic sexing strain for B. dorsalis using the temperature-sensitive lethal (tsl) mutation. This mutation causes the death of females at a certain temperature, enabling the separation of male and female fruit flies [187]. The genetic sexing strain has been proven effective in suppressing the population growth of several fruit fly species in field trials [188].…”

Section: Sterile Insect Technique (Sit)mentioning

confidence: 99%

Understanding the Invasion, Ecological Adaptations, and Management Strategies of Bactrocera dorsalis in China: A Review

Jaffar,

Rizvi,

2023

Horticulturae

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Bactrocera dorsalis (Hendel, 1912) (Diptera: Tephritidae), commonly known as the oriental fruit fly, is a highly destructive pest that globally infests fruits and vegetables, resulting in significant annual economic losses. Initially detected in Taiwan Island, it has rapidly expanded its distribution range to various regions in mainland China since the 1980s, with a continuous northward spread. To mitigate the damage caused by this pest, extensive efforts have been undertaken to comprehend its ecological and physiological adaptations and develop management strategies. This review article provides an overview of the invasion history of B. dorsalis in China, its ecological and physiological mechanisms facilitating its invasion, and the progress made in understanding its major biological characteristics. Moreover, the key approaches for managing B. dorsalis that have been or are likely to be implemented in China are presented, including quarantine measures, monitoring procedures, physical controls, biological controls, the sterile insect technique, RNA interference, and CRISPR-Cas-9. Finally, some suggestions for future research directions are provided.

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Temperature Sensitivity of Wild-Type, Mutant and Genetic Sexing Strains of Ceratitis capitata

Cited by 7 publications

References 35 publications

Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region

Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region

Probiotics as a Beneficial Modulator of Gut Microbiota and Environmental Stress for Sustainable Mass-Reared Ceratitis capitata

Understanding the Invasion, Ecological Adaptations, and Management Strategies of Bactrocera dorsalis in China: A Review

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