Templated xerogels as platforms for biomolecule-less biomolecule sensors

Fluorescent protein recognition polymer thin films capable of selective signal transduction of target binding events prepared by molecular imprinting with a post-imprinting treatment

“…Fluorescence has been frequently used as a highly sensitive technique for the detection and imaging of biologically active compounds, and naturally efforts have been made to introduce fluorescent binding sites to MIPs for the visualization of the binding events by measuring the fluorescence change (Tao et al, 2006;Kubo et al, 2005;Mitch et al, 2001). In most cases, fluorescent functional monomers were co-polymerized to construct polymer matrices, and the problem is that the polymers show high background fluorescence due to the intrinsic fluorescence of functional monomers that may be immobilized outside the specific binding cavity.…”

“…To reduce such background, a minimum amount of fluorescent reporter molecule should be introduced into MIPs, which is located only inside the molecularly imprinted cavities. For example, Tao et al (2006) has reported protein-imprinted xerogels, where an azido-fluorescent dye was conjugated near the binding sites by photoreaction.…”

Recognition of Conformational Changes in β-Lactoglobulin by Molecularly Imprinted Thin Films

“…The results presented here are modest in terms of the magnitude of their effect, when compared to the recognition obtained by certain recent advances in protein imprinting. 55,56,61 They however demonstrate the validity of the molecular Figure 9. Rebinding of native -lactoglobulin (N-BLG) in 50 mM phosphate buffer, pH 7, to an N-BLG MIP (diamonds), to 1% HFIP-BLG MIP (triangles), and to 8% HFIP-BLG MIP (squares).…”

Electrosynthesized Surface‐Imprinted Conducting Polymer Microrods for Selective Protein Recognition

“…[10] Other important strategies to generate protein MIPs involve the use of novel polymeric materials, epitope imprinting, and surface imprinting. Thus, solgel derived xerogels, [11] hydrogels, or other materials, currently in use in gel electrophoresis of proteins with smaller degree of crosslinking were implemented to reduce nonspecific binding and to enable the transport of macromolecules in the polymeric network. However, the flexibility of some of these structures might adversely affect the quality of the imprint and the stability of the MIP.…”