Temporal and spatial expression analysis of peripheral myelin protein 22 (Pmp22) in developing Xenopus

Tae, Hyun‐Jin; Rahman, Md. Mahfujur; Park, Byung-Yong

doi:10.1016/j.gep.2015.01.001

Cited by 4 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enhanced axonal growth and regeneration by isoquercitrin was also supported by the enlarged diameter and thickness of myelin sheath induced by treatment with isoquercitrin. MAG involves in myelinmaintenance and PMP22, primarily expressed by Schwann cells, contributes to the formation and maintenance of myelin sheaths (32,33,50). Our data also demonstrated that isoquercitrin promoted the expression of MAG and PMP22 compared to saline, which suggested that isoquercitrin was beneficial to remyelination via promoting the expression of MAG and PMP22 after peripheral nerve injury.…”

Section: Discussionsupporting

confidence: 65%

“…Therefore, we evaluate the expression of the myelin-associated glycoprotein (MAG) and peripheral myelin protein 22 (PMP22). MAG is implicated in the formation and maintenance of myelin (31) and PMP22 is primarily expressed in the compact myelin and promotes the formation and maintenance of myelin sheaths in the PNS (32,33). The expression of MAG and PMP22 were reduced in saline treated group compared to normal control group, which indicated that the structure and function of myelin sheath had been damaged to some extent.…”

Section: Isoquercitrin Is Beneficial For Remyelination and Nerve Regeneration After Peripheral Nerve Injurymentioning

confidence: 98%

See 1 more Smart Citation

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Qiu¹,

Yang

Wang

et al. 2019

Ann. Transl. Med.

View full text Add to dashboard Cite

Background: Oxidative stress has been recognized to play a crucial role in the pathogenesis of peripheral nerve injury. Isoquercitrin (quercetin-3-glucoside) is a flavonoid that exhibited many biological activities, including antioxidative effect. However, it is unclear whether isoquercitrin has protective effects on peripheral nerve injury.Methods: Mice treated by isoquercitrin were used as a case group, and mice injected with saline was the control group. Sciatic behavioral function was assessed using SFI and CMAPs were measured by electrophysiology.Schwann cells proliferation and migration were tested using EdU staining and Transwell migration chambers respectively. The expression of oxidative stress related factors were detected by qRT-PCR and Western blotting.Results: In present study, our results demonstrated that isoquercitrin (20 mg/kg/day) treatment achieved significantly higher SFI and higher amplitude of CMAP, promoted the nerve regeneration and remyelination, increased the production of GAP43, NF200, MAG and PMP22, alleviated target muscle atrophy and autophagy, and suppressed the expression of ATG7, PINK1 and Beclin1 in soleus muscles after sciatic nerve crush. In vitro studies found that isoquercitrin promoted the axonal regeneration of DRGs neurons, the proliferation and migration of Schwann cells, and the expression of proliferating cell nuclear antigen (PCNA) in Schwann cells. The administration of isoquercitrin at 40 and 320 μM showed a dose dependent, and high doses of isoquercitrin (160 and 320 μM) showed better performance in promoting axonal regeneration of DRGs neurons, and the proliferation and migration of Schwann cells than low dose of isoquercitrin (40 μM). Furthermore, isoquercitrin significantly inhibited oxidative stress through reducing the production of Nox4 and Duox1, and promoting the expression of Nrf2 and SOD2 in soleus muscles after sciatic nerve crush.Conclusions: Isoquercitrin may promote motor functional recovery and nerve regeneration following peripheral nerve injury though inhibition of oxidative stress, which highlighted the therapeutic values of isoquercitrin as a neuroprotective drug for peripheral nerve repair applications.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Isoquercitrin Is Beneficial For Remyelination and Nerve Regeneration After Peripheral Nerve Injurymentioning

confidence: 98%

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Qiu¹,

Yang

Wang

et al. 2019

Ann. Transl. Med.

View full text Add to dashboard Cite

show abstract

“…Peripheral myelin protein 22 (PMP22) is a disease-associated protein and contributes to peripheral myelination in the peripheral nervous system [6]. Recently, accumulated evidences suggest that PMP22 is emerging as a key player in a variety of prevalent cancers.…”

mentioning

confidence: 99%

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

Qu¹,

Zhu²,

Tao³

et al. 2015

neo

View full text Add to dashboard Cite

PMP22 is recently recognized as a key player in a variety of prevalent cancers. In this study, we sought to explore the correlation of peripheral myelin protein 22 (PMP22) expression with cell proliferation, invasion and apoptosis in lung cancer cell line. miR29 was transfected into Anip973 lung adenocarcinoma cell line to interfere the expression of PMP22 using Lipofectamine ® 2000 reagent. Real-time polymerase chain reaction (RT-PCR) and Western blot were performed to determine the expression level of PMP22 at mRNA and protein level. Then MTT, Matrigel transwell assay and flow cytometry were respectively used to explore the proliferation, invasion and apoptosis in Anip973 lung adenocarcinoma cell line in vitro. miR29 could significantly down-regulate the expression level of PMP22 in Anip973 cells not only at mRNA level but also at protein level. Moreover, the proliferation rate, invasive cell number and apoptosis rate of Anip973 cells in miR29 transfected group significantly decreased compared with blank group, while no significant difference existed between control group and blank group. Our study found that suppression of PMP22 expression could inhibit cell proliferation, invasion and apoptosis in lung cancer cells. All these findings suggest that PMP22 may be involved in progression of lung cancer and could be a new therapeutic target for this disease.

show abstract

“… 36 PMP22 is mainly expressed by Schwann cells and takes part in the formation and maintenance of myelin sheath; importantly, PMP22 is crucial in protecting nerves from physical pressure, helping restore the structure after being pinched or squeezed. 37 , 38 For example, isoquercitrin treatment significantly increases the production of GAP43, NF200, MAG, and PMP22, and promotes the nerve regeneration and remyelination. 39 Our results revealed that UTI administration increased the expression of these markers, which suggested that UTI promoted the functional recovery of peripheral nerve by facilitating the axonal outgrowth and remyelination.…”

Section: Discussionmentioning

confidence: 99%

<p>Ulinastatin Promotes Regeneration of Peripheral Nerves After Sciatic Nerve Injury by Targeting let-7 microRNAs and Enhancing NGF Expression</p>

Zhang

Chen

et al. 2020

DDDT

View full text Add to dashboard Cite

Background: Peripheral nerve injury is characterized as a common clinical problem. Ulinastatin (UTI) is a serine protease inhibitor with many biological activities including antiinflammatory and antioxidant effects. Nonetheless, it is unknown whether UTI has a protective effect on peripheral nerve injury. Methods: Thirty rats were divided into the sham operation group, the sciatic nerve injury group (injected with normal saline), and the UTI treatment group (80mg/kg/day for two consecutive weeks). Sciatic nerve function index (SFI) was used to assess the biological functions of the sciatic nerve, and compound muscle action potential (CMAP) was measured by electrophysiology. The expressions of let-7 miRNA members were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Nerve growth factor (NGF), nerve regeneration-related proteins GAP43 and NF200, and myelin formation-related proteins MAG and PMP22 expressions were explored by Western blot. After Schwann cells were transfected with let-7 mimics, pcDNA3.1-NGF, let-7 inhibitors, NGF siRNA and their corresponding controls, 5-ethynyl-2ʹ-deoxyuridine (EdU) assay, and Transwell assays were employed to investigate the proliferation and migration of Schwann cells. H 2 O 2 was utilized to construct oxidative injury to cells, and the contents of MDA, SOD, GSH, and CAT were determined. Results: UTI treatment remarkably increased SFI of the rats and CMAP of sciatic nerve, enhanced nerve regeneration, and myelin regeneration, and raised the production of GAP43, NF200, MAG, and PMP22. Furthermore, it was found that UTI markedly reduced let-7 miRNAs' expressions and increased NGF expression after sciatic nerve injury. The dualluciferase reporter assay validated that let-7 miRNAs targeted NGF, and functional experiments demonstrated that low expression of let-7 miRNAs and NGF overexpression contributed to Schwann cells' proliferation and migration. Additionally, UTI treatment repressed the oxidative stress regulated by let-7/NGF axis. Conclusion: UTI modulates the let-7/NGF axis to inhibit oxidative stress, promote nerve regeneration, and facilitate function recovery after peripheral nerve injury.

show abstract

Temporal and spatial expression analysis of peripheral myelin protein 22 (Pmp22) in developing Xenopus

Cited by 4 publications

References 33 publications

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Isoquercitrin promotes peripheral nerve regeneration through inhibiting oxidative stress following sciatic crush injury in mice

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

<p>Ulinastatin Promotes Regeneration of Peripheral Nerves After Sciatic Nerve Injury by Targeting let-7 microRNAs and Enhancing NGF Expression</p>

Contact Info

Product

Resources

About