Temporal and spatial expression of Pax9 and Sonic hedgehog during development of normal mouse palates and cleft palates in TGF-β3 null embryos

Sasaki, Yoshiro; O’Kane, Sharon; Dixon, Jill; Dixon, Michael J.; Ferguson, Mark W. J.

doi:10.1016/j.archoralbio.2006.09.012

Cited by 34 publications

(41 citation statements)

References 24 publications

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“…The expression of Bmp-2 was strong in the most medial palatal mesenchyme of the anterior region ( fig. 5 A A comparison of Shh expression between WT and Tgf-␤ 3 -/-mouse palates has already been reported [Sasaki et al, 2007]. Contrary to what was observed by these researchers, we found very little difference in Shh expression between these palates ( fig.…”

Section: The Presence Of Egf But Not Of Egf-r Fgf-10 Pdgf-a or Tgcontrasting

confidence: 57%

“…7 ). As previously described for the WT secondary palate [Sasaki et al, 2007, Pantalacci et al, 2008, high Shh expression was observed in the epithelium of the rugae and on the oral side of the future soft palate of both genotypes ( fig. 7 ).…”

Section: The Presence Of Egf But Not Of Egf-r Fgf-10 Pdgf-a or Tgmentioning

confidence: 57%

“…We and others have reported the misexpression of several growth and transcription factors in the Tgf-␤ 3 -/-mouse palate which cause some of these alterations [Martínez-Álvarez et al, 2004;Knight et al, 2006;Sasaki et al, 2007;Murillo et al, 2009]. In the present work, we hypothesized that the presence of 1 or more molecules related to cell proliferation has shifted in the Tgf-␤ 3 -/-mouse palate and that this is responsible for the change in cell proliferation.…”

Section: Introductionmentioning

confidence: 76%

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Analysis of the Presence of Cell Proliferation-Related Molecules in the Tgf-β3 Null Mutant Mouse Palate Reveals Misexpression of EGF and Msx-1

Río

Barrio

Murillo

et al. 2010

Cells Tissues Organs

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The Tgf-β₃ null mutant mouse palate presents several cellular anomalies that lead to the appearance of cleft palate. One of them concerns the cell proliferation of both the palatal medial edge epithelium and mesenchyme. In this work, our aim was to determine whether there was any variation in the presence/distribution of several cell proliferation-related molecules that could be responsible for the cell proliferation defects observed in these palates. Our results showed no difference in the presence of EGF-R, PDGF-A, TGF-β₂, Bmp-2, and Bmp-4, and differences were minimal for FGF-10 and Shh. However, the expression of EGF and Msx-1 changed substantially. The shift of the EGF protein expression was the one that most correlated with that of cell proliferation. This molecule is regulated by TGF-β₃, and experiments blocking its activity in culture suggest that EGF misexpression in the Tgf-β₃ null mutant mouse palate plays a role in the cell proliferation defect observed.

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Section: The Presence Of Egf But Not Of Egf-r Fgf-10 Pdgf-a or Tgcontrasting

confidence: 57%

Section: The Presence Of Egf But Not Of Egf-r Fgf-10 Pdgf-a or Tgmentioning

confidence: 57%

Section: Introductionmentioning

confidence: 76%

See 1 more Smart Citation

Analysis of the Presence of Cell Proliferation-Related Molecules in the Tgf-β3 Null Mutant Mouse Palate Reveals Misexpression of EGF and Msx-1

Río

Barrio

Murillo

et al. 2010

Cells Tissues Organs

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show abstract

“…Genetic studies in humans have suggested a role for Pax9 in the pathogenesis of CLP (Sasaki et al, 2007;Hamachi et al, 2003;Küchler et al, 2014), but the results have been inconclusive. PAX9-positive cell distribution varied from occasional to numerous positive structures in the patients examined, and PAX9 expression in the oral mucosa was lower in the CBCLP compared to the control group.…”

Section: Discussionmentioning

confidence: 98%

IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate

Smane

Pilmane

2015

Int. J. Morphol.

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“…38,39 There also was functional relationship between Pax9 and Msx1 during tooth development, and Pax9 may be involved in the TGF-b3 regulation of normal palatal fusion. 40,41 Previous studies reported that retinoic acid receptor a (Rara) may be a candidate gene for CL/ P, and retinoic acid also may bind to Rara to inhibit apoptosis, resulting in a failure of palatal shelves to fuse, whereas others propose that retinoic acid disrupts elevation or retards the growth of palatal shelves. 42,43 Previous studies also demonstrated that mutations of Msx1 gene in human cause CL/P and tooth agenesis.…”

Section: Discussionmentioning

confidence: 99%

Irf6-Related Gene Regulatory Network Involved in Palate and Lip Development

Dai

et al. 2015

Journal of Craniofacial Surgery

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Numerous genes including Irf6 have been revealed to contribute to cleft lip with or without cleft palate (CL/P). In this study, we performed a systematic bioinformatics analysis of Irf6-related gene regulatory network involved in palate and lip development by using GeneDecks, DAVID, STRING, and GeneMANIA database. Our results showed that many CL/P candidate genes have relation with Irf6, and 9 of these genes, including Msx1, Pvrl1, Pax9, Jag2, Irf6, Tgfb3, Rara, Gli2, and Tgfb2, were enriched into the CL/P gene group. Some of these 9 genes also were commonly involved in different signaling pathways and different biological processes, and they also have protein-protein interactions with Irf6. These findings make us analyze the intricate function of Irf6 in a CL/P gene regulatory network, followed by guiding us to perform further functional studies on these genes in the future. This method also offers us a simple, cheap, but useful method to analyze the relationship with a gene regulatory network of a certain disease such as CL/P.

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Temporal and spatial expression of Pax9 and Sonic hedgehog during development of normal mouse palates and cleft palates in TGF-β3 null embryos

Cited by 34 publications

References 24 publications

Analysis of the Presence of Cell Proliferation-Related Molecules in the <i>Tgf-</i>β<sub><i>3</i></sub> Null Mutant Mouse Palate Reveals Misexpression of EGF and <i>Msx-1</i>

Analysis of the Presence of Cell Proliferation-Related Molecules in the <i>Tgf-</i>β<sub><i>3</i></sub> Null Mutant Mouse Palate Reveals Misexpression of EGF and <i>Msx-1</i>

IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate

Irf6-Related Gene Regulatory Network Involved in Palate and Lip Development

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