Temporal changes in the cell population and wound healing-related gene expression in deep partial-thickness burn wound model

Guo, Han; Ali, Razana Mohd; Hamid, Roslida Abd; Chang, Sui Kiat; Rahman, Md. Habibur; Zainal, Zaida; Khaza’ai, Huzwah

doi:10.1186/s41702-020-0059-6

Cited by 9 publications

(10 citation statements)

References 26 publications

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“…A moderate increase in MMP-9 serum concentration is beneficial because the epithelialization rate is partially dependent on the presence of collagen. However, when the physiological balance between protease and their inhibitors is broken, the patient outcome is unfavorable (9). Our results showed an early increase in the MMP-9 level, most likely due to its rapid release from neutrophil granulocytes, over minutes to a few hours after the triggering insult (P1, 1744.19 ng/ml vs. C, 279.36 ng/ml, P<0.001).…”

Section: Discussionmentioning

confidence: 64%

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Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

et al. 2021

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No prospective study has specifically examined the serum levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the early shock phase of burn-injured patients. Thus, we aimed to detect early changes, activity dynamics, and the predictive value of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio to better understand the early repair mechanisms for the development of future therapies for patients with thermal burns. Twenty-five patients with a total body surface area (TBSA) affected by burn <25%, and 30 healthy subjects were enrolled in the study. Serum levels of MMP-9 and TIMP-1 were determined by the ELISA method. Our results showed that MMP-9 concentrations increased immediately after injury and remained on a plateau. In contrast, TIMP-1 showed an upward trend throughout the 7-day study period, and the time course of the MMP-9/TIMP-1 ratio followed the inverse dynamics of TIMP-1. Analysis of the areas under the receiver operating characteristic (ROC) curves (AUC) showed that patients with burn wounds tended to have a MMP-9 value higher than 421.5 ng/ml (AUC= 0.979), TIMP-1 value higher than 231.6 ng/ml (AUC= 0.908), and MMP-9/TIMP-1 ratio higher than 2.31 (AUC= 0.959) (P<0.001). Our findings suggest that although the variations in the two biomarkers were different regarding the time of the initial insult, their ratio is a specific and sensitive indicator of burn evolutivity in patients with a TBSA affected by a burn <25%.

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Section: Discussionmentioning

confidence: 64%

“…Simultaneously, the decrease in TIMPs leads to intense matrix proteolysis (5)(6)(7)(8). Virtually, any imbalance between MMPs and TIMPs may lead to low healing processes such as chronic wounds or excessive healing, including hypertrophic scars and keloids (9).…”

Section: Introductionmentioning

confidence: 99%

Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

et al. 2021

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“…Our results showing an increasing trend for TIMP-1 are in contrast to other studies (11,23) that have found significantly higher TIMP-1 concentrations in the plasma of patients with TBSA affected by a burn of <20% relative to healthy controls, with a median time to peak TIMP-1 concentration at 2.09 days. Given that a moderate increase in the MMP-9 serum concentration is beneficial, the epithelialization rate being partially dependent on the presence of collagen (13,16), MMP-9 levels remaining constant during the 7-day monitoring period could be associated with a better-quality scar. Because none of the patients enrolled in our study had septic complications during the 7-day monitoring period, the maintaining/decrease of MMP-9 serum levels may be an indicator for better survival.…”

Section: Discussionmentioning

confidence: 99%

“…In burn wounds, with a typical chronic evolution, degradation products can be found but it is not clear which of the tissue proteinases are responsible for local repair (10). Another possible explanation could be the degradation of the adhesion molecules and growth factors by the local proteinases (12)(13)(14). Moreover, the expression of different tissue proteases in burn wounds could influence the prognosis (15).…”

Section: Role and Dynamics Of Matrix Metalloproteinase 9 And Tissue Inhibitor Of Metalloproteinase 1 In Burn Patientsmentioning

confidence: 99%

Role and dynamics of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in burn patients

et al. 2021

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Burn injuries can trigger tissue changes that can explain the variation in the level of different biochemical markers that can be recorded both locally or systemically. Some events observed in burn wounds such as vascular hyperpermeability have been associated with the release of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) after trauma. Because it is unknown whether the serum levels of MMP-9 and TIMP-1 are a consequence of these destructions or a local response to thermal damage, we decided to follow their dynamics. Twenty-five patients (mean age 49.40±17.55 years) with a total body surface area (TBSA) affected by a thermal burn of <25% and 30 healthy subjects (mean age 49.70±8.04 years) were enrolled in the present study. Enzyme immunoassays were used to measure the serum levels of MMP-9 and TIMP-1. Our results showed that MMP-9 was increased 6.25-fold immediately after injury compared to the controls and remained on a plateau throughout the 7-day monitoring period. TIMP-1 showed an upward trend with an increase of 49.52% on the seventh day after triggering insult. The time-course of the MMP-9/TIMP-1 ratio followed the inverse dynamics of TIMP-1 starting from a ratio value measured at admission 3.82-fold higher than the one observed in the healthy volunteers and a highly statistically significant correlation between the values measured at different time-points during the monitoring period (P<0.001). The results of this retrospective study indicate that the MMP-9/TIMP-1 ratio may provide information on local changes over time, starting from the triggering insult, and may be considered as a predictive biomarker of burn evolutivity.

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“…Burns are a very common skin injury. Depending on the depth of thermal damage, burns are divided into three main categories: superficial burns, which affect only the epidermis, partial-thickness burns, in which both the epidermis and dermis are affected, and burns of full-thickness skin (Guo et al, 2020). Deep thermal burns are characterized by the development of general and local disorders that lead to a complex of interconnected processes in all body systems (Vaghardoost et al, 2019;Sterling & Lombardi, 2021).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of extracts from cryopreserved placenta on third-degree burns in rats

Naumenko

Shchetinskey

Bobrova

et al. 2021

Regul. Mech. Biosyst.

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Human placenta extracts have anti-inflammatory, antioxidant and wound-healing properties, so they are promising drugs for the treatment of wounds of various origins, including burns. Cryopreservation methods are widely used to preserve the biological activity of placental drugs for a long time. The aim of this work was to study the effect of low-temperature storage of the placenta on the regenerative properties of its extracts. Fragments of freshly obtained placentas were cooled by immersion in liquid nitrogen and stored at –196 °C for 6 months. The placenta was warmed in a water bath at 37 °C. The effect of low-temperature preservation of the placenta on the ability of its extracts to positively affect the wound healing process was studied in a model of thermal burn of III B degree in rats. The effectiveness of wound treatment with extracts from cryopreserved placenta was evaluated by planimetric and histological methods at 3, 7, 14, 21 and 28 days after the burn. The activity of antioxidant enzymes in the serum of animals was also determined. superoxide dismutase activity was assessed by inhibition of adrenaline autooxidation in carbonate buffer, catalase activity was assessed by the degree of inhibition of ammonium peroxide formation. It has been shown that the treatment of burns with extracts from cryopreserved placenta helped to accelerate the regeneration processes and the rate of wound healing. The formation of granulation tissue was detected on the 7th day of treatment with extracts, and on the 14th day in the control. The area of burn wounds during treatment with extracts probably differed from the control starting from 14 days after application of the burn. It was found that the dynamics of recovery of catalase activity after burns is probably higher on the 7th day of treatment with extracts. The obtained data testify to the high efficiency of application of placenta stored at low-temperature for the purpose of obtaining extracts from it with preservation of regenerative properties.

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Temporal changes in the cell population and wound healing-related gene expression in deep partial-thickness burn wound model

Cited by 9 publications

References 26 publications

Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution

Role and dynamics of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in burn patients

Efficacy of extracts from cryopreserved placenta on third-degree burns in rats

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