Ten Years of CRISPRing Cancers In Vitro

Abstract

Cell lines have always constituted a good investigation tool for cancer research, allowing scientists to understand the basic mechanisms underlying the complex network of phenomena peculiar to the transforming path from a healthy to cancerous cell. The introduction of CRISPR in everyday laboratory activity and its relative affordability greatly expanded the bench lab weaponry in the daily attempt to better understand tumor biology with the final aim to mitigate cancer’s impact in our lives. In this review, we aim to report how this genome editing technique affected in the in vitro modeling of different aspects of tumor biology, its several declinations, and analyze the advantages and drawbacks of each of them.

Benzenesulfonamide decorated dihydropyrimidin(thi)ones: carbonic anhydrase profiling and antiproliferative activity

“…Cancer is one of the leading causes of mortality worldwide 1,2 and a disease that mostly impacts negatively on the quality of life of the patients and their families along with the public costs. 3 The entire scientific community, both the pharma industry and academia, is frantically searching for new therapeutical options through the application of recently developed technologies, e.g., CRISPR gene editing, 4,5 robotic surgery, 6 personalized medicine, 7 and the generation of new drugs from chemical, natural, and biotechnological sources. 8 Undoubtedly, the most chosen approach is based on the discovery and validation of new putative pharmacological targets, e.g.…”