Tendon Healing In Vivo: Gene Expression and Production of Multiple Growth Factors in Early Tendon Healing Period

Chen, Chuan Hao; Cao, Yi; Wu, Ya Fang; Bais, Anthony J.; Gao, Jianxi; Tang, Jin Bo

doi:10.1016/j.jhsa.2008.07.003

Cited by 138 publications

(114 citation statements)

References 39 publications

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“…Heparin conjugation with electrospun polymeric fibres with subsequent PDGF-BB loading ( Fig. 5A) has been done in only a few studies using poly (ε-caprolactone) (PCL)/gelatin fibres (Lee et al, 2012b), poly(L-lactide) fibres (plasma assisted heparin conjugation) (Cheng et al, 2014) and PCL/gelatin electrospun fibres (Lee et al, 2012a;Lee et al, 2012b), where cellular bioactivity and cell infiltration were studied.…”

Section: Heparin-conjugated Systemsmentioning

confidence: 99%

“…As the endogenous release of PDGF-BB is during the inflammatory and early proliferative phase (Chen et al, 2008;Gulotta and Rodeo, 2009;Wuergler-Hauri et al, 2007), it should be administered within the first two weeks after injury, at best using a delivery method that allows a controlled and sustained release. So far, clinical use of PDGF-BB in the tendon repair field has not been reported, with the main issue represented by its administration, i.e.…”

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

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To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques.In this review, the structure and composition, as well as the healing process of tendons, are described. Part A deals with in vitro studies. They focus on the multiple effects evoked by PDGF-BB on the cellular level. Moreover, they address strategies for the sustained delivery of PDGF-BB. Part B focuses on animal models used to test different delivery strategies for PDGF-BB, in the context of tendon reconstruction. These studies showed that dosage and timing of PDGF-BB application are the most important factors for deciding which delivery device should be applied for a specific tendon laceration.

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Section: Heparin-conjugated Systemsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

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“…14 CTGF has previously been shown to be expressed by tenocytes, 15 and undergoes upregulation following injury. 16 CTGF has also been proposed as an attractive target to decrease scarring following tendon injury. 17 Thus, the reduction of CTGF expression in response to SC injections may represent a potential benefit of this treatment.…”

Section: Gene Expression Changes Mediated By Mast Cell Degranulationmentioning

confidence: 99%

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Sharma

Abraham

Sampaio

et al. 2010

Journal Orthopaedic Research

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The purpose of this study was to determine whether administration of a mast cell inhibitor (sodium cromolyn, SC) would influence tendon repair and extracellular matrix gene expression following acute injury. CD1 mouse patellar tendons were unilaterally injured and mast cell prevalence was determined. The effect of SC injection on tendon hypercellularity, cross-sectional area, collagen organization, and expression of extracellular matrix-related genes was examined. Mast cell prevalence was markedly increased in injured patellar tendons ( p ¼ 0.009), especially at 8 weeks post-injury ( p ¼ 0.025). SC injection increased collagen organization compared to uninjected animals at 4 weeks and attenuated the development of tendon hypercellularity and tendon thickening post-injury. Expression of CTGF, ADAMTS1, and TIMP3 in injured tendon was reduced in the SC group. SC injections moderated the structural alterations of healing tendon in association with downregulation of several genes associated with tendon fibrosis. This work corroborates previous findings pointing to a role of mast cells in tendon repair. ß

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“…In contrast, several animal studies have demonstrated up-regulation of the IGF-1 expression during tendon healing [6,7,9]. This up-regulation was seen both during an early and a later phase of healing.…”

Section: Introductionmentioning

confidence: 91%

Growth Hormone Does Not Stimulate Early Healing in Rat Tendons

2012

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Growth Hormone stimulates bone growth and fracture repair. It acts mainly by increasing the systemic levels of IGF-1. Local treatment with IGF-1 appears to stimulate tendon healing.We therefore hypothesized that systemic treatment with Growth Hormone would also stimulate tendon healing. Rat Achilles tendons were transected and left to heal. Four groups were studied. Intramuscular injections of botulinum toxin A (Botox) were used to reduce loading in 2 groups. The animals were randomized to twice daily injections of Growth Hormone (n = 2 x 10) or saline (n = 2 x 10), and killed after 10 days. Healing was assessed by mechanical testing. Muscle paralysis induced by Botox reduced the strength of the healing tendon by two thirds. Growth Hormone increased femoral and tibial length in the unloaded, and femoral and tibial weight in the loaded group. Body weight and muscle weight were increased in both. In contrast, there was no increase in the strength of the healing tendons, regardless of mechanical loading status. An increase in peak force of the loaded healing tendons by more than 5 % could be excluded with 95 % confidence. In spite of its stimulatory effects on other tissues, Growth Hormone did not appear to stimulate tendon or tendon repair.

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Tendon Healing In Vivo: Gene Expression and Production of Multiple Growth Factors in Early Tendon Healing Period

Cited by 138 publications

References 39 publications

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Growth Hormone Does Not Stimulate Early Healing in Rat Tendons

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