Tenebrionibacter intestinalis gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.

Abstract

A Gram-negative, white-pigmented, motile and rod-shaped strain, BIT-L3T, was isolated from the gut of plastic-eating mealworm Tenebrio molitor L. Its taxonomic position was determined by using a polyphasic approach. A preliminary analysis based on the 16S rRNA gene sequence (1445 bp) revealed that this strain was closely related to the members within the family Enterobacteriaceae . Phylogenetic trees based on the concatenated partial sequences of seven housekeeping genes (atpD, gyrB, infB, rpoB, pyrG, fusA, leuS) and genome sequences further showed that strain BIT-L3T constituted a separate lineage within the family Enterobacteriaceae. In silico DNA–DNA hybridization values and average nucleotide identity values between strain BIT-L3T and its closest related species within the family Enterobacteriaceae were less than 21.8 and 76.7 %, respectively. The major fatty acids (>5 %) of strain BIT-L3T were C16 : 0, C14 : 0, C17 : 0 cyclo, summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c and/or iso-C15 : 0 2-OH) and summed feature 2 (comprising iso-C16 : 1 I/C14 : 0 3-OH and/or C12 : 0 aldehyde and/or an unknown fatty acid of equivalent chain length 10.9525). Its genomic DNA G+C content was 53.7 mol%. Based on the results of phylogenetic, physiological and biochemical analyses, strain BIT-L3T is considered to represent a novel species of a novel genus within the family Enterobacteriaceae , for which the name Tenebrionibacter intestinalis gen. nov., sp. nov. is proposed. The type strain is BIT-L3T (=CCTCC AB 2020371T=LMG 32222T=TBRC 14825T).

Apirhabdus apintestini gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the western honey bee Apis mellifera

“…Data for Tenebrionicola larvae YMB-R21 T (GCA_019148575.1) was obtained from Lee et al [68]. Data for Tenebrionibacter intestinalis BIT-L3 T (GCA_016632365.1) was obtained from Hu and Yang [67]. +, Positive result; −, negative result; nd, no data; /+, expected positive result based on genome sequences; /−, expected negative result based on genome sequences.…”

“…The taxonomic novelty of strain CA-0114 T at the genus level within the Enterobacteriaceae was also confirmed using the GTDB taxonomic classifier version 1.7.0 software [ 65 ] (topology and ANI method) as implemented in the KBase server [ 66 ]. Interestingly, we found that the AAI, OrthoANIu, dDDH and 16S rRNA identity values between Tenebrionibacter intestinalis BIT-L3 T and Tenebrionicola larvae YMB-R21 T were alarmingly high (99.64, 99.83, 98.20 and 99.24 %, respectively) which suggests that these species are subjective synonyms [ 67 68 ].…”

Biodegradation of vulcanized rubber by a gut bacterium from plastic-eating mealworms

“…For taxonomic identification of the obtained isolates, genomic DNA extraction, PCR amplification, and sequencing of the 16 S rRNA gene were carried out according to the previously described method [20,21]. The closest phylogenetic neighbors and the corresponding similarity were determined by aligning the obtained 16 S rRNA sequence against the bacterial type species recorded in the EzBioCloud database [22].…”