Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo

Kenzelmann, D.; Chiquet‐Ehrismann, Ruth; Leachman, Nathaniel T.; Tucker, Richard P.

doi:10.1186/1471-213x-8-30

Cited by 61 publications

(89 citation statements)

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“…We previously used antisera raised against the ICD and EGFlike repeats of teneurin-1 on immunoblots of embryonic chicken brain (Kenzelmann et al 2008). The anti-teneurin-1 ICD antibodies recognized a band running at approximately 64kDa, and the antiserum directed to the EGF-like repeats of teneurin-1 recognized larger bands corresponding to the full length protein.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleus mesencephalicus lateralis pars dorsalis is also labeled. In the E17 cerebellum, which is a prominent site of expression for other teneurins (e.g., Kenzelmann et al 2008), teneurin-4 immunostaining is faint (not shown). This corresponds with the faint band seen in homogenates of the E17 cerebellum (Fig.…”

Section: Immunolocalization Of Teneurin-4 In the Chick Embryomentioning

confidence: 99%

“…Other teneurins can be processed by proteolytic cleavage between the transmembrane domain and the tenascin-type EGFlike repeats, which may be the first step leading to release of the ICD to translocate to the nucleus (e.g., Kenzelmann et al 2008). To see if teneurin-4 is similarly processed, antisera were raised against the ICD and the EGF-like repeats found in the ECD of Homogenates of embryonic day 17 telencephalon (TEL), retina (RET), optic tectum (OT) and cerebellum (CBL) were separated by SDS-PAGE, transferred to filter and probed with the anti-ICD and anti-EGF sera.…”

Section: Evidence Of Teneurin-4 Processingmentioning

confidence: 99%

“…Note that the immunostaining of the developing limbs was transient: the anti-teneurin-4 did not stain limb buds prior to E3, and the staining of the limbs is lost between E4 and E5. We previously described the expression of teneurin-1 and -2 in the E17 central retina (Kenzelmann et al 2008): teneurin-1 is found in the retinal ganglion cell layer, inner plexiform layer and outer nuclear layer, and within the inner plexiform layer it is most prominently expressed in two laminae (Fig. 5E); teneurin-2 is found in the inner plexiform layer and the outer plexiform A second teneurin-1 antiserum raised against an intracellular domain epitope gives the same staining pattern, but is nuclear (inset).…”

Section: G B C D E F Hmentioning

confidence: 99%

“…When the ICD of teneurin-1 (Nunes et al 2005) or teneurin-2 (Bagutti et al 2003) is expressed in vitro it localizes to the cell nucleus, and antibodies to the ICD of avian teneurin-1 label some cell nuclei in a subset of tissues stained by antibodies to the ECD (Kenzelmann et al 2008). When a potential nuclear localization signal in the ICD of teneurin-1 is mutated, the ICD fails to translocate to the nucleus (Kenzelmann et al 2008). However, genes that are regulated by the ICD of teneurins have yet to be identified.…”

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confidence: 99%

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The expression of teneurin-4 in the avian embryo: potential roles in patterning of the limb and nervous system

et al. 2010

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Teneurins are type II transmembrane proteins that play important roles in pattern formation in Drosophila, axon fasciculation and organogenesis in Caenorhabidits elegans, and neuronal pathfinding in the visual system of the mouse. There is evidence that a peptide derived from a proteolytic event near the C-terminus of teneurins leads to formation of an active neuropeptide, while processing at and near the transmembrane domain leads to shedding of the extracellular domain into the extracellular matrix and the generation of an intracellular fragment that is transported to the nucleus. In vertebrates there are four teneurins. Here, we have studied the expression of teneurin-4 in the chicken embryo. An antiserum against part of the intracellular domain of teneurin-4 recognizes several low molecular weight bands on immunoblots of embryonic chicken brain homogenates, indicating that teneurin-4 is likely to be processed at one or more predicted proteolytic cleavage sites. Antisera against the EGF-like repeats of the extracellular domain label some mesenchyme in the early embryo, and near basement membranes this labeling partially overlaps with anti-laminin (gamma 1 chain) immunostaining. At embryonic day 7, anti-teneurin-4 labels bundles of axons in the nasal, but not temporal retina. Later in development, retinal expression switches so that teneurin-4 is found in the temporal, but not nasal, ganglion cell layer. Teneurin-4 immunolocalization was also compared with other teneurins in the developing limb, where each teneurin is expressed in distinctive regions. These patterns of expression suggest roles for teneurin-4 in patterning and neuronal pathfinding in the avian embryo.

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Section: Discussionmentioning

confidence: 99%

Section: Immunolocalization Of Teneurin-4 In the Chick Embryomentioning

confidence: 99%

Section: Evidence Of Teneurin-4 Processingmentioning

confidence: 99%

Section: G B C D E F Hmentioning

confidence: 99%

mentioning

confidence: 99%

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The expression of teneurin-4 in the avian embryo: potential roles in patterning of the limb and nervous system

et al. 2010

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The role of cell adhesion molecules in visual circuit formation: From neurite outgrowth to maps and synaptic specificity

Missaire

Hindges

2015

Developmental Neurobiology

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The formation of visual circuitry is a multistep process that involves cell–cell interactions based on a range of molecular mechanisms. The correct implementation of individual events, including axon outgrowth and guidance, the formation of the topographic map, or the synaptic targeting of specific cellular subtypes, are prerequisites for a fully functional visual system that is able to appropriately process the information captured by the eyes. Cell adhesion molecules (CAMs) with their adhesive properties and their high functional diversity have been identified as key actors in several of these fundamental processes. Because of their growth‐promoting properties, CAMs play an important role in neuritogenesis. Furthermore, they are necessary to control additional neurite development, regulating dendritic spacing and axon pathfinding. Finally, trans‐synaptic interactions of CAMs ensure cell type‐specific connectivity as a basis for the establishment of circuits processing distinct visual features. Recent discoveries implicating CAMs in novel mechanisms have led to a better general understanding of neural circuit formation, but also revealed an increasing complexity of their function. This review aims at describing the different levels of action for CAMs to shape neural connectivity, with a special focus on the visual system. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 569–583, 2015

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Ten‐m4 plays a unique role in the establishment of binocular visual circuits

Young

Black

Mansuri

et al. 2023

Developmental Neurobiology

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The patterning of binocular vision requires distinct molecular pathways for inputs arising from each side of the nervous system. Recent studies have demonstrated important roles for members of the Ten‐m/Odz/teneurin family in the development of ipsilateral retinal projections. Here, we further highlight the significance of this gene family in visual development by identifying a role for Ten‐m4 during the formation of the ipsilateral projection in the mouse. Ten‐m4 was found to be expressed in the retina, dorsal lateral geniculate nucleus (dLGN), superior colliculus (SC), and primary visual cortex (V1) during development. Anterograde and retrograde tracing experiments in Ten‐m4 knockout (KO) mice revealed a specific increase in ipsilateral retinal ganglion cells projecting to dLGN and SC. This increase was most prominent in regions corresponding to temporal retina. Consistent with this, EphB1 expression in the retina around the time of decussation was enhanced in this temporal region for KO mice, suggesting that the increased size of the ipsilateral population arises due to an increased number of retinal ganglion cells remaining ipsilaterally at the optic chiasm due to EphB1‐mediated repulsion. The ectopic ipsilaterally targeted retinal ganglion cell projection observed in Ten‐m4 KOs was associated with changes in response to ethologically relevant visual stimuli. Together, these data demonstrate a requirement for Ten‐m4 in the establishment of ipsilateral projections from the retina, which likely acts in combination with other Ten‐m members (Ten‐m2 and Ten‐m3) to promote the formation of functional binocular circuits.

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Teneurin-1 is expressed in interconnected regions of the developing brain and is processed in vivo

Abstract: Background: Teneurins are a unique family of transmembrane proteins conserved from C. elegans and D. melanogaster to mammals. In vertebrates there are four paralogs (teneurin-1 to -4), all of which are expressed prominently in the developing central nervous system.

Cited by 61 publications

References 42 publications

The expression of teneurin-4 in the avian embryo: potential roles in patterning of the limb and nervous system

The expression of teneurin-4 in the avian embryo: potential roles in patterning of the limb and nervous system

The role of cell adhesion molecules in visual circuit formation: From neurite outgrowth to maps and synaptic specificity

Ten‐m4 plays a unique role in the establishment of binocular visual circuits

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