Tenidap, an Anti-Inflammatory Agent, Inhibits DNA and Collagen Syntheses, Depresses Cell Proliferation, and Lowers Intracellular pH in Cultured Human Gingival Fibroblasts

Matsumoto, Hiroko; Fujii, Akira

doi:10.1124/jpet.300.2.668

Cited by 14 publications

(8 citation statements)

References 27 publications

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“…Pulmonary stretch receptors (PSRs) became more active with increasing lung volume V (membrane bias = 0.5 V mV/%VC) and mediated the Hering–Breuer reflex. Deflation-sensitive lung receptors were also implemented (Paintal, 1955; Luck, 1970; Wei and Shen, 1985; Iscoe and Gordon, 1992; Bergren and Peterson, 1993; Matsumoto et al, 2002; Yu et al, 2003). A low threshold population (Def_1, membrane bias = − 0.225( V − 70) mV/%VC) and its afferent pathway introduced in Poliaček et al (2011) was used to represent a class of possible network mechanisms for generating an inhibitory bias on E-Dec neurons.…”

Section: Resultsmentioning

confidence: 99%

A Joint Computational Respiratory Neural Network-Biomechanical Model for Breathing and Airway Defensive Behaviors

O'Connor¹,

Segers²,

Morris³

et al. 2012

Front. Physio.

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Data-driven computational neural network models have been used to study mechanisms for generating the motor patterns for breathing and breathing related behaviors such as coughing. These models have commonly been evaluated in open loop conditions or with feedback of lung volume simply represented as a filtered version of phrenic motor output. Limitations of these approaches preclude assessment of the influence of mechanical properties of the musculoskeletal system and motivated development of a biomechanical model of the respiratory muscles, airway, and lungs using published measures from human subjects. Here we describe the model and some aspects of its behavior when linked to a computational brainstem respiratory network model for breathing and airway defensive behavior composed of discrete "integrate and fire" populations. The network incorporated multiple circuit paths and operations for tuning inspiratory drive suggested by prior work. Results from neuromechanical system simulations included generation of a eupneic-like breathing pattern and the observation that increased respiratory drive and operating volume result in higher peak flow rates during cough, even when the expiratory drive is unchanged, or when the expiratory abdominal pressure is unchanged. Sequential elimination of the model's sources of inspiratory drive during cough also suggested a role for disinhibitory regulation via tonic expiratory neurons, a result that was subsequently supported by an analysis of in vivo data. Comparisons with antecedent models, discrepancies with experimental results, and some model limitations are noted.

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Section: Resultsmentioning

confidence: 99%

A Joint Computational Respiratory Neural Network-Biomechanical Model for Breathing and Airway Defensive Behaviors

O'Connor¹,

Segers²,

Morris³

et al. 2012

Front. Physio.

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“…4 Decreased failure loads and increased rates of failure have also been found in surgically repaired rat supraspinatus tendons of animals given NSAIDs compared with controls. 5 In studies using in vitro cell cultures, NSAIDs have been reported to inhibit collagen synthesis in various cell types 6,7 and therefore are postulated to exert a negative impact on tissue healing.…”

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confidence: 99%

Ibuprofen upregulates expressions of matrix metalloproteinase-1, -8, -9, and -13 without affecting expressions of types I and III collagen in tendon cells

et al. 2009

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Nonsteroidal antiinflammatory drugs are widely used to treat sports-related tendon injuries or tendinopathy. This study was designed to investigate the effect of ibuprofen on expressions of types I and III collagen, as well as collagen-degrading enzymes including matrix metalloproteinase (MMP)-1, -2, -8, -9, and -13. Rat Achilles tendon cells were treated with ibuprofen and then underwent MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Reverse transcription-polymerase chain reaction was used to evaluate mRNA expressions of types I and III collagen, MMP-1, -2, -8, -9, and -13. Protein expressions of types I and III collagen, MMP-1, -8, and -13 were determined by Western blot analysis. Gelatin zymography was used to evaluate the enzymatic activities of MMP-2 and MMP-9. The results revealed that ibuprofen upregulated expressions of MMP-1, -8, -9, and -13, both at mRNA and protein levels. There was no effect of ibuprofen on mRNA and protein expressions of types I and III collagen. Gelatin zymography revealed that the enzymatic activity of MMP-9 was upregulated after ibuprofen treatment. In conclusion, ibuprofen upregulates the expressions of collagenases including MMP-1, -8, -9, and -13 without affecting the expressions of types I and III collagen. These findings suggest a molecular mechanism potentially accounting for the inhibition of tendon healing by ibuprofen. ß

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“…Hsu et al [10] reported that saikosaponin D inhibited the growth of cells in the human cancer cell line, A549. Matsumoto and Fujii [11] reported that tenidap, an anti-inflammatory agent, inhibited collagen synthesis in cultured human gingival fibroblasts. Thus, we propose that depression of both bFGF exocytosis and collagen synthesis as a result of steroidal effect of saikosaponin and glycyrrhizin in gingival fibroblasts is involved in the characteristic growth-inhibiting effect of Saireito.…”

Section: Discussionmentioning

confidence: 99%

Therapy for nifedipine-induced gingival overgrowth by saireito in rats

et al. 2009

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ObjectiveA calcium antagonist, nifedipine, causes gingival overgrowth as a side effect. It has been confirmed that the Japanese traditional medicine, Saireito, inhibits the nifedipine-induced proliferation of gingival fibroblasts in vitro. We performed an in vivo experiment to determine whether Saireito has a therapeutic use in the treatment of nifedipine-induced gingival overgrowth.MethodsThe rats had significant gingival overgrowth induced by the administration of nifedipine. The space between the submandibular incisors and the width of buccal gingiva of maxillary left first molar were macroscopically measured. The buccal gingiva was microscopically examined.ResultsEight weeks after Saireito was administrated together with nifedipine, Saireito decreased both the incisor space and the gingiva width which had been enlarged by nifedipine and furthermore, the area of connective tissue of nifedipine + Saireito group was significantly smaller than that of nifedipine alone.ConclusionIn conclusion, Saireito may be clinically effective in therapy for calcium antagonist-induced gingival overgrowth.

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Tenidap, an Anti-Inflammatory Agent, Inhibits DNA and Collagen Syntheses, Depresses Cell Proliferation, and Lowers Intracellular pH in Cultured Human Gingival Fibroblasts

Cited by 14 publications

References 27 publications

A Joint Computational Respiratory Neural Network-Biomechanical Model for Breathing and Airway Defensive Behaviors

A Joint Computational Respiratory Neural Network-Biomechanical Model for Breathing and Airway Defensive Behaviors

Ibuprofen upregulates expressions of matrix metalloproteinase-1, -8, -9, and -13 without affecting expressions of types I and III collagen in tendon cells

Therapy for nifedipine-induced gingival overgrowth by saireito in rats

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