Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons

Yeh, Chih Ko; Rodan, Gideon A.

doi:10.1007/bf02406136

Cited by 171 publications

(54 citation statements)

References 19 publications

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“…As Ca 2þ i mobilization is an early intracellular signalling event involved in bone mechanotransduction, and ALP is a marker for bone mineralization and ECM deposition, these are good indicators of the anabolic effect of mechanical stimulus. Past studies applying large strains (more than 10 000 m1) have also observed increased nitric oxide (NO) and prostaglandin (PG) production, both essential for an osteogenic response, at these strain levels [49,50]. In contrast, more recent studies have suggested that production of NO and PGs, as well as mRNA expression of the important bone remodelling protein osteopontin, may be more closely linked with loading-induced fluid flow than direct substrate stimulation [9,48,51].…”

Section: Discussionmentioning

confidence: 99%

Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes

Verbruggen

Vaughan

McNamara

2012

J. R. Soc. Interface.

134

104

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The osteocyte is believed to act as the main sensor of mechanical stimulus in bone, controlling signalling for bone growth and resorption in response to changes in the mechanical demands placed on our bones throughout life. However, the precise mechanical stimuli that bone cells experience in vivo are not yet fully understood. The objective of this study is to use computational methods to predict the loading conditions experienced by osteocytes during normal physiological activities. Confocal imaging of the lacunar -canalicular network was used to develop three-dimensional finite element models of osteocytes, including their cell body, and the surrounding pericellular matrix (PCM) and extracellular matrix (ECM). We investigated the role of the PCM and ECM projections for amplifying mechanical stimulation to the cells. At loading levels, representing vigorous physiological activity (3000 m1), our results provide direct evidence that (i) confocal image-derived models predict 350 -400% greater strain amplification experienced by osteocytes compared with an idealized cell, (ii) the PCM increases the cell volume stimulated more than 3500 m1 by 4 -10% and (iii) ECM projections amplify strain to the cell by approximately 50 -420%. These are the first confocal image-derived computational models to predict osteocyte strain in vivo and provide an insight into the mechanobiology of the osteocyte.

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Section: Discussionmentioning

confidence: 99%

Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes

Verbruggen

Vaughan

McNamara

2012

J. R. Soc. Interface.

134

104

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“…It has been reported that the synthesis of prostaglandins by cultured osteoblasts isolated from rat calvaria is increased upon stretching (25). It is not known whether ICF causes mouse chondrocytes (the main body of the cells in a fetal long bone) to increase their synthesis of prostaglandins.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of osteoclastic bone resorption by mechanical stimulation in vitro

Klein‐Nulend

Veldhuijzen

Strien

et al. 1990

Arthritis & Rheumatism

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The influence of mechanical stimulation by intermittent compressive force (ICF) of physiologic magnitude on osteoclastic bone resorption was investigated in cultures of fetal mouse cartilaginous long bones. Exposure to ICF resulted in a significant decrease in mineral resorption, as indicated by the decreased release of %a and a decreased number of osteoclasts in the diaphysis. Conditioned medium (CM) from ICF-exposed periosteum-free cultures (ICF-CM), but not from control cultures (Co-CM), inhibited mineral resorption in fresh bones cultured under control conditions. Co-CM increased, but ICF-CM decreased, the number of tartrate-resistant acid phosphatase-positive cells in 7-day bone marrow cultures. Direct exposure of bone marrow cultures to ICF yielded the same results. Thus, osteoclastic bone resorption in cartilaginous long bones is inhibited by ICF in vitro. A soluble factor($ acting on tartrate-resistant acid phosphatase-positive, osteoclast precursor-like cells seems to play a role in this effect.Mechanical forces play an important role in the differentiation, growth, and remodeling of the skeleton. An overall decrease in the functional load exerted on the skeleton produces mineral loss and osteoporo-

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“…mechanical load (5). The effect of mechanical load on PGE2 synthesis have already been documented (6,7).…”

mentioning

confidence: 93%

Shape Changes of Osteoblastic Cells Under Gravitational Variations during Parabolic Flight. Relationship with PGE2 Synthesis.

Guignandon¹,

Vico²,

Alexandre³

et al. 1995

Cell Struct. Funct.

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Key words: osteoblasts/parabolic flight/PGE2/microgravity/hypergravity ABSTRACT. The relationship existing between cell morphology and cell metabolism, and the role of mechanical load in bone remodelling are well-known. In osteoblasts, PGE2mediates part of the response to mechanical stress and induce cell shape changes. Westudied the influence of gravity variations on osteoblast morphology and its relationship with PGE2 synthesis during a parabolic flight. ROS17/2.8 osteosarcoma cells flew 15 or 30 parabolae. Wemeasured cell area and shape factor after fluorescein staining with a semi-automatic image analyser and PGE2levels by RIA. Significant flight-induced shape changes consisted in a decrease in cell area and an increase in shape factor (cell irregularity), in some cells, as compared to ground controls. This heterogeneity in cell response might be explained by a cell-cycle sensitivity to mechanical stress. A 45 min pretreatment with indomethacin inhibited the flight-induced increase in cell irregularity whereas cell area remained decreased. PGE2 levels were higher in flight than in ground controls. Linear regression analysis showed a significant negative relationship between cell area and PGE2 synthesis. We concluded that ROS 17/2.8 are highly sensitive to gravitational variations and that PGE2is partly implicated in cell shape changes observed during parabolic flight. However, other mechanisms than PGE2 synthesis condition ROS17/2.8 morphology in response to mechanical changes.

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Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons

Cited by 171 publications

References 19 publications

Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes

Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes

Inhibition of osteoclastic bone resorption by mechanical stimulation in vitro

Shape Changes of Osteoblastic Cells Under Gravitational Variations during Parabolic Flight. Relationship with PGE2 Synthesis.

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