Tension transduction device for functional evaluation of the rat peroneal nerve

Spyropoulos, Basil P.; Santos, Perry M.; Williams, Sherri L.

doi:10.1016/0165-0270(94)90149-x

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…Tension transduction device. Eighteen rats underwent surgical reexposure of the nerves described above at week 13 for TTD 15 . Four rats were not tested due to technical difficulties, and two rats were lost due to anesthesia complications.…”

Section: Methodsmentioning

confidence: 99%

Brain‐Derived Neurotrophic Factor and Peripheral Nerve Regeneration: A Functional Evaluation

1996

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The potential benefit of brain-derived neurotrophic factor (BDNF) on motor-nerve regeneration after transection injury in 24 adult rats was evaluated after entubulation repair. Gait analysis for ankle angle and tension transduction device (TTD) strain-gauge measurements yielded functional evaluation of regeneration. The BDNF (15 mg/mL) or phosphate buffered saline (control) was injected into the silicone elastomer (Silastic) channel. Gait analysis performed 0, 2, 4, 6, 10, and 12 weeks after injury demonstrated a significant difference between uninjured and injured legs of 23 and 43 degrees, respectively (P<.001, analysis of variance). The TTD evaluation 13 weeks after injury demonstrated a significant decrease in force development of injured compared to uninjured legs, 148 and 58 g, respectively (P<.001). No functional benefits were demonstrated between BDNF-treated versus control-treated animals in either model for a single exposure to BDNF.

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Section: Methodsmentioning

confidence: 99%

Brain‐Derived Neurotrophic Factor and Peripheral Nerve Regeneration: A Functional Evaluation

1996

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“… 21 TTD testing requires surgical exposure and induces some scarring; therefore, re‐testing is not practical, since force development progressively decreases. 20 The finding of an HBO‐related benefit was measured with the gait analysis during the earliest phase of recovery. Other studies have also shown an HBO benefit under conditions of hypoxia but not with gait analysis or TTD functional models.…”

Section: Discussionmentioning

confidence: 99%

“…Tension transduction device methods and materials are previously described but briefly reviewed with additional modifications. 20 After surgical re‐exposure of the peroneal nerve in injured limbs, the knee is rested on a bumper, and the foot is mounted to a footplate by means of an external adjustable clamp. The metatarsals, ankle, and knee are positioned so that planar motion of dorsiflexion is oriented parallel to the tabletop and perpendicular to the footplate.…”

Section: Methodsmentioning

confidence: 99%

A Functional Model System of an Hypoxic Nerve Injury and Its Evaluation

Santos

2000

The Laryngoscope

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Objectives/Hypothesis: Develop an hypoxic peripheral nerve injury model with a controlled injury type and two types of clinically relevant physiological measurements of function during and after recovery. The model, controlling for injury and measurement variables, would have predictable outcomes in function. The functional model could test potential therapeutic interventions with greater sensitivity. Study Design: Twenty-one rats were used in preliminary studies evaluating peroneal nerve injury types and functional model evaluation. Forty-eight rats were used in a controlled and blinded evaluation of the injury model followed by treatment with hyperbaric oxygen (HBO) as a potential therapeutic intervention and evaluated with functional models. Methods: Preliminary studies compared nerve injuries: epineurectomy, epineurectomy with crush and transection with autograft for rate of return of function and final extent of return of function. The gait analysis model was also evaluated and modified to decrease variability. The final study evaluated peroneal epineurectomy and nerve crush injury with serial gait analysis during recovery, final elicited maximum force measurements, and histological analysis. Half of the animals were treated with HBO during recovery (ANOVA or regression statistical analysis were used to determine group differences.) Results: Preliminary studies suggested that the peroneal nerve injury model of an epineurectomy with crush of specified length and a modification of the gait analysis model would yield a useful and predictable injury outcome. The final study resulted in predicted and consistent injury outcomes. In the HBO treatment group, a 12% improvement in function 5 days after HBO treatment was demonstrated (P < .03), but no long-term or histological benefit was seen. Conclusion: A reliable hypoxic nerve injury model has been developed and tested utilizing two functional methods as the primary outcome variables.

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“…), measured force is also used as an essential parameter to quantify the degree of nerve regeneration after injury (Spyropoulos et al. ). Further it is possible to gain objective data to assess the influence of pathologic conditions on the ability to generate force (Edwards ; Gibson et al.…”

Section: Introductionmentioning

confidence: 99%

In-situ measurements of tensile forces in the tibialis anterior tendon of the rat in concentric, isometric, and resisted co-contractions

et al. 2017

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Tensile‐force transmitted by the tibialis anterior (TA) tendon of 11 anesthetized adult male Wistar rats (body‐mass: 360.6 ± 66.3 g) was measured in‐situ within the intact biomechanical system of the hind‐limb using a novel miniature in‐line load‐cell. The aim was to demonstrate the dependence of the loading‐profile experienced by the muscle, on stimulation‐frequency and the resistance to shortening in a group of control‐animals. Data from these acute‐experiments shows the type of loading achievable by means of implantable electrical stimulators activating agonists or agonist/antagonist groups of muscles during programmed resistance‐training in freely moving healthy subjects. Force‐responses to electrical stimulation of the common peroneal nerve for single pulses and short bursts were measured in unloaded and isometric contractions. A less time‐consuming approach to measure the force‐frequency relationship was investigated by applying single bursts containing a series of escalating stimulus‐frequencies. We also measured the range of loading attainable by programmed co‐contraction of the TA‐muscle with the plantar‐flexor muscles for various combinations of stimulation‐frequencies. The maximal average peak‐force of single twitches was 179% higher for isometric than for unloaded twitches. Average maximal isometric tetanic‐force per gramme muscle‐mass was 16.5 ± 3.0 N g−1, which agrees well with other studies. The standard and time‐saving approaches to measure the force‐frequency relationship gave similar results. Plantar‐flexor co‐activation produced greatly increased tension in the TA‐tendon, similar to isometric contractions. Our results suggest that unloaded contractions may not be adequate for studies of resistance‐training. Plantar‐flexor co‐contractions produced considerably higher force‐levels that may be better suited to investigate the physiology and cell‐biology of resistance‐training in rodents.

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Tension transduction device for functional evaluation of the rat peroneal nerve

Cited by 10 publications

References 9 publications

Brain‐Derived Neurotrophic Factor and Peripheral Nerve Regeneration: A Functional Evaluation

Brain‐Derived Neurotrophic Factor and Peripheral Nerve Regeneration: A Functional Evaluation

A Functional Model System of an Hypoxic Nerve Injury and Its Evaluation

In-situ measurements of tensile forces in the tibialis anterior tendon of the rat in concentric, isometric, and resisted co-contractions

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