Tepidimonas fonticaldi sp. nov., a slightly thermophilic betaproteobacterium isolated from a hot spring

Chen, Wen Ming; Huang, Hsing Wei; Chang, Jo Shu; Han, Yin Lung; Guo, Tai Rong; Sheu, Shih Yi

doi:10.1099/ijs.0.043729-0

Cited by 26 publications

(20 citation statements)

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“…This bacterium grows aerobically on organic acids (acetate, lactate, and pyruvate) and amino acids within a broad temperature range from 35 to 60°C (with the optimum at 55°C), at low ambient salinity and neutral pH, and is capable of nitrate reduction (Chen et al, 2013). No data are presently available concerning its ability to utilize components of crude oil.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

et al. 2017

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The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations. At this water-flooded oilfield, the microbial community of formation water from the near-bottom zone of an injection well where the most active microbial processes of oil degradation occur was investigated using molecular, cultural, radiotracer, and physicochemical techniques. The samples of microbial DNA and RNA from back-flushed water were used to obtain the clone libraries for the 16S rRNA gene and cDNA of 16S rRNA, respectively. The DNA-derived clone libraries were found to contain bacterial and archaeal 16S rRNA genes and the alkB genes encoding alkane monooxygenases similar to those encoded by alkB-geo1 and alkB-geo6 of geobacilli. The 16S rRNA genes of methanogens (Methanomethylovorans, Methanoculleus, Methanolinea, Methanothrix, and Methanocalculus) were predominant in the DNA-derived library of Archaea cloned sequences; among the bacterial sequences, the 16S rRNA genes of members of the genus Geobacillus were the most numerous. The RNA-derived library contained only bacterial cDNA of the 16S rRNA sequences belonging to metabolically active aerobic organotrophic bacteria (Tepidimonas, Pseudomonas, Acinetobacter), as well as of denitrifying (Azoarcus, Tepidiphilus, Calditerrivibrio), fermenting (Bellilinea), iron-reducing (Geobacter), and sulfate- and sulfur-reducing bacteria (Desulfomicrobium, Desulfuromonas). The presence of the microorganisms of the main functional groups revealed by molecular techniques was confirmed by the results of cultural, radioisotope, and geochemical research. Functioning of the mesophilic and thermophilic branches was shown for the microbial food chain of the near-bottom zone of the injection well, which included the microorganisms of the carbon, sulfur, iron, and nitrogen cycles.

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Section: Discussionmentioning

confidence: 99%

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

et al. 2017

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“…Tepidimonas taiwanensis and Tepidimonas fonticaldi are both thermophilic bacteria isolated from a hot spring of southern Thailand (Chen et al 2006) and Antun hot spring from Taiwan (Chen et al 2013). This study also identified few Tepidimonas OTUs in Jakrem 16S rRNA library.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and archeal community composition in hot springs from Indo-Burma region, North-east India

et al. 2016

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Bacterial and archaeal diversity of two alkaline Indian hot springs, Jakrem (Meghalaya) and Yumthang (Sikkim), were studied. Thirteen major bacterial phyla were identified of which Firmicutes, Chloroflexi and Thermi were dominant in Jakrem and Proteobacteria in Yumthang. The dominant genera were Clostridium, Chloroflexus and Meiothermus at Jakrem (water temperature 46 °C, pH 9) and Thiobacillus, Sulfuritalea at Yumthang (water temperature 39 °C, pH 8) hot springs. The four Euryarchaeota taxa that were observed in both the hot springs were Methanoculleus, Methanosaeta, Methanosarcina and Methanocorposculum. Elstera litoralis, Thiovirga sp., Turneriella sp. were observed for the first time in association with hot springs along with Tepidibacter sp., Ignavibacterium sp., Teribacillus sp. and Dechloromonas sp. Individual bacterial phyla were found to be specifically correlated with certain physico-chemical factors such as temperature, dissolved SiO2, elemental S, total sulphide, calcium concentrations in hot spring water. Bacterial reads involved in sulfur cycle were identified in both16S rRNA gene library and sulfur metabolism may play key physiological functions in this hot spring. Members within Desulfobacterales and Thermodesulfovibrionaceae were identified and hypothesized their role in regulating sulfur cycle. The presence of many taxonomically unsolved sequences in the 16S rRNA gene tag datasets from these hot springs could be a sign of novel microbe richness in these less known hot water bodies of Northeastern India.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0284-y) contains supplementary material, which is available to authorized users.

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“…T. ignava SPS-1037 T and T. sediminis YIM 72259 T have genes coding for the nitrate/nitrite transporters nasA/narK but the nitrate reductase complex narGHIJ was not identified in the genome sequences. Nitrate did not appear to be reduced by T. thermarum AA-1 T experimentally in one study but his strain has been reported to reduce nitrate in another study [4,5]. However, the genome predicts that nitrate should be reduced to nitrite because this organism possesses narGHIJ.…”

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confidence: 97%

“…html# tepidimonas). The genus comprises seven species with validly published names, Tepidimonas ignava [1], Tepidimonas aquatica [2], Tepidimonas taiwanensis [3], Tepidimonas thermarum [4] and Tepidimonas fonticaldi [5], Tepidimonas sediminis and Tepidimonas alkaliphilus [6], while the species 'Tepidimonas arfidensis' [7] has not been validly published.…”

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“…The genome sequences of the organisms used in this study clarified the likely reasons why the type strain of T. taiwanensis is able to use glucose and fructose while the other strains are not (Table S1, available in the online version of this article). The genome analysis indicated that glucose and fructose transporters only occur in the type strain of T. taiwanensis, as well as strains MB2 and VT154-175, where putative ABC glucose/mannose (gtsABCD) and Experimental nitrate reduction to nitrite has been observed for the type strains of T. aquatica, T. fonticaldi, T. thermarum and T. taiwanensis but not or the type strain of T. ignava and strain SPSP-6 T [5]. The strains of the members of the genus Tepidimonas have variable genes involved in nitrogen metabolism (Table S2).…”

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Comparative genome sequence analysis of several species in the genus Tepidimonas and the description of a novel species Tepidimonas charontis sp. nov.

Albuquerque

Castelhano

Raposo

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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We performed high-quality genome sequencing of eight strains of the species of the genus Tepidimonas and examined the genomes of closely related strains from the databases to understand why Tepidimonas taiwanensis is the only strain of this genus that utilizes glucose and fructose for growth. We found that the assimilation of these hexoses by T. taiwanensis was due to the presence of two transporters that are absent in all other genomes of strains of members of the genus Tepidimonas examined. Some strains lack genes coding for glucokinase, but the Embden–Meyerhof–Parnas pathway appears to be otherwise complete. The pentose phosphate pathway has a complete set of genes, but genes of the Entner–Doudoroff pathway were not identified in the genomes of any of the strains examined. Genome analysis using average nucleotide identity (ANIb), digital DNA–DNA hybridization (dDDH), average amino acid identity (AAI) and phylogenetic analysis of 400 conserved genes was performed to assess the taxonomic classification of the organisms. Two isolates of the genus Tepidimonas from the hot spring at São Pedro do Sul, Portugal, designated SPSP-6T and SPSPC-18 were also examined in this study. These organisms are mixotrophic, have an optimum growth temperature of about 50 ºC, utilize several organic acids and amino acids for growth but do not grow on sugars. Distinctive phenotypic, 16S rRNA gene sequence and genomic characteristics of strains SPSP-6T and SPSPC-18 lead us to propose a novel species based on strain SPSP-6T for which we recommend the name Tepidimonas charontis sp. nov. (=CECT 9683T=LMG 30884T).

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Tepidimonas fonticaldi sp. nov., a slightly thermophilic betaproteobacterium isolated from a hot spring

Cited by 26 publications

References 26 publications

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir

Bacterial and archeal community composition in hot springs from Indo-Burma region, North-east India

Comparative genome sequence analysis of several species in the genus Tepidimonas and the description of a novel species Tepidimonas charontis sp. nov.

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