Tepidiphilus olei sp. nov., isolated from the production water of a water-flooded oil reservoir in PR China

Wang, Xiaotong; Shan, Jian-Jie; Li, Xizhe; Lin, Wei; Xiu, Jianlong; Li, Dong-An; Zhang, Yun-Zeng; Wang, Lei

doi:10.1099/ijsem.0.004297

Cited by 12 publications

(4 citation statements)

References 33 publications

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“…are known as probiotics that are able to produce lactic acid during food fermentation. − Some Weissella spp., such as W. cibaria , are currently receiving notable attention as potential probiotics in the field of periodontics . There are only four species in Tepidiphilus , which are commonly detected in geothermal environments . This is the first report of Tepidiphilus from Fuzhuan brick tea.…”

Section: Resultsmentioning

confidence: 94%

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Dynamic Changes in the Microbial Community and Metabolite Profile during the Pile Fermentation Process of Fuzhuan Brick Tea

Le,

Zhong,

Ren

et al. 2023

J. Agric. Food Chem.

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The pile fermentation process of Fuzhuan brick tea is unique in that it involves preheating without the use of starter cultures. The detailed metabolite changes and their drivers during this procedure are not known. Characterizing these unknown changes that occur in the metabolites and microbes during pile fermentation of Fuzhuan brick tea is important for industrial modernization of this traditional fermented food. Using microbial DNA amplicon sequencing, mass spectrometry-based untargeted metabolomics, and feature-based molecular networking, we herein reveal that significant changes in the microbial community occur before changes in the metabolite profile. These changes were characterized by a decrease in Klebsiella and Aspergillus, alongside an increase in Bacillus and Eurotium. The decrease in lysophosphatidylcholines, unsaturated fatty acids, and some astringent flavan-3-ols and bitter amino acids, as well as the increase in some less astringent flavan-3-ols and sweet or umami amino acids, contributed importantly to the overall changes observed in the metabolite profile. The majority of these changes was caused by bacterial metabolism and the corresponding heat generated by it.

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Section: Resultsmentioning

confidence: 94%

“…25 There are only four species in Tepidiphilus, which are commonly detected in geothermal environments. 26 This is the first report of Tepidiphilus from Fuzhuan brick tea.…”

Section: Diversities Of the Microbial Community Changedmentioning

confidence: 87%

Dynamic Changes in the Microbial Community and Metabolite Profile during the Pile Fermentation Process of Fuzhuan Brick Tea

Le,

Zhong,

Ren

et al. 2023

J. Agric. Food Chem.

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“…Seven of them were isolated from marine water, and the rest two were from production water ( Tepidiphilus sp. J18 [ 67 ]) and soil ( Xenophilus azovorans DSM 13,620 [ 68 ]). Despite the enrichment of these genera observed in PAH-contaminated sites [ 69 , 70 ], related PAH-degrading isolates have not yet been reported, probably owing to the isolation bottleneck.…”

Section: Resultsmentioning

confidence: 99%

Polycyclic aromatic hydrocarbon (PAH) biodegradation capacity revealed by a genome-function relationship approach

Huang

Yin

et al. 2023

Environmental Microbiome

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Background Polycyclic aromatic hydrocarbon (PAH) contamination has been a worldwide environmental issue because of its impact on ecosystems and human health. Biodegradation plays an important role in PAH removal in natural environments. To date, many PAH-degrading strains and degradation genes have been reported. However, a comprehensive PAH-degrading gene database is still lacking, hindering a deep understanding of PAH degraders in the era of big data. Furthermore, the relationships between the PAH-catabolic genotype and phenotype remain unclear. Results Here, we established a bacterial PAH-degrading gene database and explored PAH biodegradation capability via a genome-function relationship approach. The investigation of functional genes in the experimentally verified PAH degraders indicated that genes encoding hydratase-aldolase could serve as a biomarker for preliminarily identifying potential degraders. Additionally, a genome-centric interpretation of PAH-degrading genes was performed in the public genome database, demonstrating that they were ubiquitous in Proteobacteria and Actinobacteria. Meanwhile, the global phylogenetic distribution was generally consistent with the culture-based evidence. Notably, a few strains affiliated with the genera without any previously known PAH degraders (Hyphomonas, Hoeflea, Henriciella, Saccharomonospora, Sciscionella, Tepidiphilus, and Xenophilus) also bore a complete PAH-catabolic gene cluster, implying their potential of PAH biodegradation. Moreover, a random forest analysis was applied to predict the PAH-degrading trait in the complete genome database, revealing 28 newly predicted PAH degraders, of which nine strains encoded a complete PAH-catabolic pathway. Conclusions Our results established a comprehensive PAH-degrading gene database and a genome-function relationship approach, which revealed several potential novel PAH-degrader lineages. Importantly, this genome-centric and function-oriented approach can overcome the bottleneck of conventional cultivation-based biodegradation research and substantially expand our current knowledge on the potential degraders of environmental pollutants.

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“…The genus Tepidiphilus has so far been described as aerobic, although one strain has been isolated from production water of an oil reservoir (Wang et al . 2020 ; Zhang et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Indigenous microbial communities in heavy oil show a threshold response to salinity

Voskuhl

Akbari

Müller

et al. 2021

FEMS Microbiology Ecology

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Microbial degradation influences the quality of oil resources. The environmental factors that shape the composition of oil microbial communities are largely unknown because most samples from oil fields are impacted by anthropogenic oil production, perturbing the native ecosystem with exogenous fluids and microorganisms. We investigated the relationship between formation water geochemistry and microbial community composition in undisturbed oil samples. We isolated 43 microliter-sized water droplets naturally enclosed in the heavy oil of the Pitch Lake, Trinidad and Tobago. The water chemistry and microbial community composition within the same water droplet were determined by ion chromatography and 16S rRNA gene amplicon sequencing, respectively. The results revealed a high variability in ion concentrations and community composition between water droplets. Microbial community composition was mostly affected by chloride concentration, which ranged from freshwater to brackish-sea water. Remarkably, microbial communities did not respond gradually to increasing chloride concentration but showed a sudden change to less diverse and uneven communities when exceeding a chloride concentration of 57.3 mM. The results reveal a threshold-regulated response of microbial communities to salinity, offering new insights into the microbial ecology of oil reservoirs.

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Tepidiphilus olei sp. nov., isolated from the production water of a water-flooded oil reservoir in PR China

Cited by 12 publications

References 33 publications

Dynamic Changes in the Microbial Community and Metabolite Profile during the Pile Fermentation Process of Fuzhuan Brick Tea

Dynamic Changes in the Microbial Community and Metabolite Profile during the Pile Fermentation Process of Fuzhuan Brick Tea

Polycyclic aromatic hydrocarbon (PAH) biodegradation capacity revealed by a genome-function relationship approach

Indigenous microbial communities in heavy oil show a threshold response to salinity

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