Terahertz coherent anti-Stokes Raman scattering microscopy

Ren, Lei; Hurwitz, Ilan; Raanan, Dekel; Oulevey, Patric; Oron, Dan; Silberberg, Yaron

doi:10.1364/optica.6.000052

Cited by 29 publications

(16 citation statements)

References 23 publications

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“…This simplified setup is smaller, cheaper, and easier-to-operate. In comparison with our previous work [ 15 ], less excitation energy is needed to excite the FWM signals and the imaging speed is much faster. This method might find significant application in tracking nanodrugs in vivo [ 21 ].…”

Section: Resultsmentioning

confidence: 84%

“…However, Silberberg’s research group implemented a single-beam CARS microscope by using the pulse shaping method [ 13 ]. Later, the team further simplified the single-beam CARS microscope system by replacing the pulse shaper with a resonant photonic crystal plate or notch filter [ 14 , 15 ]. Recently, researchers found that the resonant photonic crystal plate or notch filter can be removed from the system by using the ultra-steep filter, proving the simplest CARS microscope to date [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Simplified Near-Degenerate Four-Wave-Mixing Microscopy

et al. 2021

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Four-wave-mixing microscopy is widely researched in both biology and medicine. In this paper, we present a simplified near-degenerate four-wave-mixing microscopy (SNDFWM). An ultra-steep long-pass filter is utilized to produce an ultra-steep edge on the spectrum of a femtosecond pulse, and a super-sensitive four-wave-mixing (FWM) signal can be generated via an ultra-steep short-pass filter. Compared with the current state-of-the-art FWM microscopy, this SNDFWM microscopy has the advantages of simpler experimental apparatus, lower cost, and easier operation. We demonstrate that this SNDFWM microscopy has high sensitivity and high spatial resolution in both nanowires and biological tissues. We also show that the SNDFWM microscopy can achieve an ultra-sensitive detection based on the electron-resonance effect. This method might find an important application in tracking of nano drugs in vivo.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Simplified Near-Degenerate Four-Wave-Mixing Microscopy

et al. 2021

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“…In other studies, CARS has also been useful in looking at cell differentiation, such as skeletal regeneration and ostegogenic differentiation in stem cells [135,136], skin cancer classification [137] and nerve cell imaging [138]. Recently, a terahertz-CARS has been developed, utilising low vibrational frequencies that are not generally observed in CARS imaging [139]. The detection of lower frequencies enables chemical characterisation, just as with standard CARS.…”

Section: Coherent Anti-stokes Raman Scatteringmentioning

confidence: 99%

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Hilzenrat

Gill

McArthur

2022

Journal of Biophotonics

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The past decade has seen an increasing demand for more complex, reproducible and physiologically relevant tissue cultures that can mimic the structural and biological features of living tissues. Monitoring the viability, development and responses of such tissues in real‐time are challenging due to the complexities of cell culture physical characteristics and the environments in which these cultures need to be maintained in. Significant developments in optics, such as optical manipulation, improved detection and data analysis, have made optical imaging a preferred choice for many three‐dimensional (3D) cell culture monitoring applications. The aim of this review is to discuss the challenges associated with imaging and monitoring 3D tissues and cell culture, and highlight topical label‐free imaging tools that enable bioengineers and biophysicists to non‐invasively characterise engineered living tissues.

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“…A polarization-resolved experiment (P-CARS) can bring additional information and even better resolution [51]. There are also some benefits that are connected with alteration of the light's wavelength, such as broadband CARS [52] and Terahertz CARS [53].…”

Section: Coherent Anti-stokes Raman Scattering (Cars)mentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

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This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

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Terahertz coherent anti-Stokes Raman scattering microscopy

Cited by 29 publications

References 23 publications

Simplified Near-Degenerate Four-Wave-Mixing Microscopy

Simplified Near-Degenerate Four-Wave-Mixing Microscopy

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Raman Scattering: From Structural Biology to Medical Applications

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