1997
DOI: 10.1007/s001250050636
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Teratogenic effect of diabetic serum is prevented by supplementation of superoxide dismutase and N-acetylcysteine in rat embryo culture

Abstract: Diabetic embryopathy and growth retardation are approximately 2-3 times more common in infants of diabetic women than in offspring of non-diabetic pregnancy [1]. The mechanisms causing these developmental disturbances are unknown [2,3]. Experimental studies in vivo and in vitro have suggested that glucose and b -hydroxybutyrate cause growth retardation and malformations in rodent embryos [4][5][6]. In an earlier study we showed that serum from insulin-treated diabetic rats is teratogenic despite normalisation … Show more

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Cited by 105 publications
(77 citation statements)
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“…The first is an increased supply of nutrients to the fetus in obese women. In animal models, an excess of several types of fuels (glucose, ketone bodies, NEFA, amino acids) was embryotoxic [25], probably acting through increased oxidative stress [26]. A second possible mechanism is hyperinsulinaemia itself.…”
Section: Resultsmentioning
confidence: 99%
“…The first is an increased supply of nutrients to the fetus in obese women. In animal models, an excess of several types of fuels (glucose, ketone bodies, NEFA, amino acids) was embryotoxic [25], probably acting through increased oxidative stress [26]. A second possible mechanism is hyperinsulinaemia itself.…”
Section: Resultsmentioning
confidence: 99%
“…Multivitamin regimens could protect from such effects, but not if begun after the first pregnancy month (Botto et al, 2000). In cultured rat embryos, NTDs induced by diabetic serum and spontaneously occurring situs inversus were both shown to be preventable by addition of superoxide dismutase to the medium (Lander and Brown, 1994;Wentzel et al, 1997). Moreover, in a set of combined in utero and in vitro mouse studies, maternal diabetes, excess glucose in the medium, and the oxidant stress inducer, antimycin A, were all found to inhibit Pax-3 expression, induce neuralcrest cell apoptosis, and cause neural tube failures.…”
Section: Discussionmentioning
confidence: 99%
“…Afterward, the embryos were dissected out of their yolk sacs and examined under a stereo microscope. The malformation score of the embryos was determined by assigning 0, 1, 5, or 10 points, respectively, to no, minor, less severe, or severe malformation (31). A malformation score of 0 indicated a completely normal embryo, fully rotated with a closed neural tube.…”
Section: Methodsmentioning
confidence: 99%