Terminal and subterminal oxidation of n-alkanes by molds

Pelz, Benjamin; Rehm, Hans‐Jürgen

doi:10.1007/bf00425013

Cited by 24 publications

(5 citation statements)

References 19 publications

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“…P. putida P8 was isolated from phenol-contaminated wastewater (3). The strain was cultivated in a minimal medium which contained (per liter): sodium succinate (4 g), (NH4)2SO4 (1.06 g), MgSO4 (0.1 g), KCl (0.74 g), K2HPO4 (2 g), FeSO4 (1.6 mg), and 1 ml of a trace element solution (27). The pH was set to 6.0 before sterilization.…”

Section: Methodsmentioning

confidence: 99%

Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity

Heipieper

Diefenbach

Keweloh

1992

Appl Environ Microbiol

360

176

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A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of sublethal concentrations of phenol in the medium with changes in the fatty acid composition of the lipids, thereby increasing the degree of saturation. At phenol concentrations which completely inhibited the growth of P. putida, the cells were still able to increase the content of the trans unsaturated fatty acid and simultaneously to decrease the proportion of the corresponding 9-cis-hexadecenoic acid. This conversion of fatty acids was also induced by 4-chlorophenol in nongrowing cells in which the de novo synthesis of lipids had stopped, as shown by incorporation experiments with labeled acetate. The isomerization of the double bond in the presence of chloramphenicol indicates a constitutively operating enzyme system. The cis-to-trans modification of the fatty acids studied here apparently is a new way of adapting the membrane fluidity to the presence of phenols, thereby compensating for the elevation of membrane permeability induced by these toxic substances.

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Section: Methodsmentioning

confidence: 99%

Conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading Pseudomonas putida P8 from substrate toxicity

Heipieper

Diefenbach

Keweloh

1992

Appl Environ Microbiol

360

176

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“…For isolation of PAH-degrading microorganisms, contaminated soil from a former coking plant was shaken with 0.28% Na2P407 for 1 h and after sedimentation of the soil particles the supernatant was diluted and plated on mineral salt agar that contained phenanthrene (100 mg/l) as sole carbon and energy source. The mineral salt medium contained (per litre deionized water) 0.8 g K2HPO4, 0.2 g KH2PO4, 1.0g NHaC1, 0.2g MgSOa'7H20, 0.1g CaCI2.2H20, 0.1 g NaCI, 0.01 g FeSOa.7H20, 1 ml trace element solution (Pelz and Rehm 1973), 1 ml vitamin solution (Kirk 1978) and for production of agar plates 15 g agar-agar. The pH was adjusted to 7.0 by adding 1 M HC1.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil

Keuth

Rehm

1991

Appl Microbiol Biotechnol

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Degradation of phenanthrene by Arthrobacter polychromooenes isolated from a contaminated soil was investigated. In experiments in which [9-14C]-phenanthrene was incubated with cultures of A. polychromooenes containing 150 mg phenanthrene/1 it was shown that after 26 h of incubation 47.7% of the recovered radiolabelled carbon originally present was metabolized to 14CO2, 47.8% was recovered from the aqueous fraction, and 4.5% remained in the dichloromethane fraction. Increasing phenanthrene concentration in the culture medium resulted in improved growth and degradation, rates, probably due to the higher amount of phenanthrene crystals in the medium. Shifting the temperature from 30°C to 35°C did not influence phenanthrene degradation significantly but inhibited cell division of A. polychromogenes. Medium supplementation with glucose led to stimulation of phenanthrene degradation at low amounts of glucose (0.45 g/l) whereas at higher concentrations (3 g/l) phenanthrene mineralization decreased.

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“…Whether hydrocarbon-utilizing yeasts produce more or less lipid than when grown with glucose as the carbon source varies with the strain and environmental conditions. It has also been shown that certain filamentous fungi degrade alkanes (Pelz and Rehm, 1973;Cerniglia and Perry, 1974;Gerasinova et al, 1975). For example, certain Mucorales fungi such as Absidia spinosa, Cunninghamella echinulata, and Mortierella isabellina metabolize C13 to C17 n-alkanes in the terminal and subterminal positions (Hoffmann and Rehm, 1976;1978).…”

Section: And 28)mentioning

confidence: 99%