Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil

Keuth, Sylvia; Rehm, Hans‐Jürgen

doi:10.1007/bf00169354

Cited by 67 publications

(26 citation statements)

References 20 publications

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“…This implies that estimation of specific bacterial growth rates on poorly soluble substrates in batch culture is often not very reliable. The observation that the specific growth rate increases with increasing amounts of (solid) substrate (Keuth and Rehm 1991) can be explained by the mechanism proposed in this paper. It is often recognized that the results of soil remediation plants, when expressed in terms of a residual concentration of polluting components, in casu PAH, do not match those of laboratory experiments.…”

supporting

confidence: 58%

Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Volkering

Breure

Sterkenburg

et al. 1992

Appl Microbiol Biotechnol

191

119

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supporting

confidence: 58%

Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Volkering

Breure

Sterkenburg

et al. 1992

Appl Microbiol Biotechnol

191

119

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“…The method described by T h e m & Fritsche (1995) using the surfactant Sapogenat T-300 as additive was not suitable for selective enrichment of PAH degraders since the detergent could be alternatively utilized as carbon and energy source. The minimal medium described by Kastner et al (1994) was used because it allowed optimal growth of different PAHdegrading reference organisms in comparison to other minimal media tested (Weissenfels et al, 1990;Keuth & Rehm, 1991).…”

Section: Isolation and Taxonomic Affiliation Of The Isolated Pah Degrmentioning

confidence: 99%

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Moser²,

et al. 1999

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Bacteria with ability to degrade polyaromatic hydrocarbons (PAHs), isolated from wastewater and soil samples, were investigated for their taxonomic, physiological and genetic diversity. Eighteen isolates able to metabolize naphthalene or phenanthrene as sole carbon source were taxonomically affiliated to different subclasses of the Proteobacteria (Sphingomonas spp., Acidovorax spp., Comamonas spp. and Pseudomonas spp.) and to phyla of Gram-positive bacteria with low and high DNA G+C content (Paenibacillus sp. and Rhodococcus spp., respectively). Representatives of the genera Pseudomonas and Sphingomonas formed a remarkably high fraction of these isolates; 9 out of 18 strains belonged to these groups. Tests for enzyme activities showed that the majority of the isolates growing with PAHs as sole sources of carbon and energy had an active catechol2,3-dioxygenase (C230). C230 specific activities were very diverse, ranging from 0-1 to 650 mU (mg protein)-'. Pseudomonas and Sphingomonas strains showed considerably higher activities than the other isolates. All PAH degraders were examined for the presence of an initial PAH dioxygenase and C230, which catalyse key steps of PAH degradation, by PCR amplification of gene fragments and subsequent hybridization. PCR primers and internal oligonucleotide probes were developed for the specific detection of the genes of Pseudomonas and Sphingomonas strains. (1996). The aim of the present investigation was to isolate bacterial PAH degraders from aquatic a n d terrestrial sites, to determine their taxonomic affiliation and to characterize them physiologically a n d genetically with respect to their ability to degrade PAHs. al., 1996) are specific for most genuine pseudornonads, distinct P. putida strains, and the genera Comamonas and Acidovorax, respectively. Keywords METHODSFurther examination of the isolates was done by physiological tests as reported previously (Kampfer et al., 1991). Fatty acid methyl ester (FAME) patterns were used as a further marker for identification. Preparation of FAME extracts, gas chromatographic analysis and numerical identification by the MIDI system (Sherlock 2.11) were performed as described previously (Kampfer et al., 1996).Determination of naphthalene and phenanthrene mineralization potential. The potential of the isolated bacteria for mineralization of PAHs was examined by gas chromatographically quantifying remaining PAH and sirnultaneously monitoring bacterial growth in batch cultures.For each tested strain, five airtight flasks were each filled with 10 rnl basal medium containing either 005 ' / o naphthalene or phenanthrene and were inoculated with approximately 10' cells. Cell number was estimated by measuring the optical density at 600nm, Remaining PAH in the cultures was extracted by vigorously shaking (30 rnin) with 5 rnl n-hexane for gas chromatographic analysis. The gas chromatograph HP 6890 was equipped with a flame-ionization detector and a 25 m capillary column (Ultra 2, cross-linked 5 % phenyl methyl siloxan ; Hewlett Packard) ....

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“…Studies have been performed on enhancing the degradation rates of PAHs by using surfactants to increase bioavailability [3,16], and the rates of PAH degradation at concentrations above their solubility limits have been examined [1,12,25].…”

Section: Introductionmentioning

confidence: 99%

Bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene

Shuttleworth

Cerniglia

1996

Microb Ecol

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Phenanthrene-degrading bacteria were isolated from enrichment cultures of soils contaminated with creosote and jet fuel. The isolates from the creosote enrichments were classified by fatty acid methyl ester profiles as Acidovorax delafieldii and Sphingomonas paucimobilis; the bacterium from the jet fuel-contaminated soil was not identified and was designated strain JFD 11. All three isolates used phenanthrene as a sole carbon and energy source, and two of the isolates used fluoranthene as a sole carbon and energy source. Anthracene and fluorene were cometabolized by all three strains, but pyrene was not transformed. Naphthalene inhibited all of the strains, and 28-h cultures ofA. delafieldii were inhibited by naphthalene concentrations as low as 5 ppm.Short-term degradation experiments were undertaken with center-well flasks and concentrations of phenanthrene ranging from 1.2 to 12.0 IxM. Since initial degradation rates were not a function of phenanthrene concentration, it was inferred that the half-saturation constants were less than the lowest phenanthrene concentration tested.

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Biodegradation of phenanthrene by Arthrobacter polychromogenes isolated from a contaminated soil

Cited by 67 publications

References 20 publications

Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Microbial degradation of polycyclic aromatic hydrocarbons: effect of substrate availability on bacterial growth kinetics

Differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using PCR and gene probes

Bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene

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