Tessaracoccus antarcticus sp. nov., a rhodopsin-containing bacterium from an Antarctic environment and emended description of the genus Tessaracoccus

Abstract

A Gram-stain-positive, facultatively anaerobic bacterium, strain JDX10T, was isolated from a soil sample of Fildes Peninsula, Antarctica. Cells of the strain were irregular rod-shaped and non-motile. Cells grew at 4–40 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, 7.5) and with 0.0–3.0 % (w/v) NaCl (optimum, 1.0 %). According to phylogenetic analysis based on 16S rRNA gene sequences, strain JDX10T was associated with the genus Tessaracoccus , and showed highest similarities to Tessaracoccus rhinocerotis CCTCC AB 2013217T (97.2 %), Tessaracoccus flavescens SST-39T (96.9 %) and Tessaracoccus terricola JCM 32157T (96.9 %). The average nucleotide identity scores of strain JDX10T to T. rhinocerotis CCTCC AB 2013217T and T. bendigoensis JCM 13525T were 74.8 and 73.3 %, respectively and the Genome-to-Genome Distance Calculator scores were 19.2 and 18.7 %, respectively. The major (>10.0 %) cellular fatty acid was anteiso-C15 : 0. The predominant isoprenoid quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The phylogenetic analysis and physiological and biochemical data showed that strain JDX10T should be classified as representing a novel species in the genus Tessaracoccus , for which the name Tessaracoccus antarcticus sp. nov. is proposed. The type strain is JDX10T (=MCCC 1H00351T=KCTC 49242T).

Tessaracoccus caeni sp. nov., a novel member of Propionibacteriaceae isolated from activated sludge in Hefei, PR China

“…Bacterial cells were observed by transmission electron microscopy (HT7700, Hitachi) using cells that were negatively stained with 1 % (w/v) phosphotungstic acid and attached to a copper mesh. Motility was determined using the hanging-drop method and gliding motility was observed on motility agar (R2A with 0.3 % agar) [14]. The range and optimum pH for growth were determined by comparing the OD 600 and wet weight of strain HF-7 T in R2A medium at pH 3–11 (at intervals of 0.5 pH unit).…”

Brooklawnia propionicigenes sp. nov., a facultatively anaerobic, propionate-producing bacterium isolated from a methanogenic reactor treating waste from cattle farms

“…The most closely related species to the four strains was B. cerclae BL-34 T [8] isolated from chlorosolvent-contaminated groundwater. As for strain SH051 T (1 468 bp of 16S rRNA gene sequence), the sequence similarity to B. cerclae BL-34 T was 96.4 % and to the next closely related species they were Tessaracoccus lapidicaptus IPBSL-7 T (95.4 %) [15], Arachnia propionica DSM 43307 T (95.2 %) [16, 17] and Tessaracoccus antarcticus JDX10 T (95.1 %) [18]. These values were lower than the recommended threshold for species designation (98.7 %) [19].…”

Tessaracoccus coleopterorum sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus

“…Strain HDW20 T grew on MA, trypticase soy agar (Bacto), and Gifu anaerobic medium (Nissui; GAM) agar. The temperature conditions suitable for growth were determined in GAM broth at 0, 4,10,15,20,25,30,37,40,45, 55 and 65 °C. Bacterial growth under different pH conditions was determined at pH 4-11 (at intervals of 1.0 pH unit) by adjusting the pH of trypticase soy broth (TSB) using 10 mM 2-(N-morpholino) ethanesulfonic acid for pH 4-6, 10 mM N-Tris (hydroxymethyl)-methyl-3-aminopropanesulfonic acid for pH 7-8, and 10 mM Na 2 HPO 4 for pH 9-11.…”

“…ʟʟ-Diaminopimelic acid is the diagnostic cell wall peptidoglycan amino acid, MK-9 or MK-10 is the major isoprenoid quinone, and anteiso-C 15 : 0 is the major fatty acid; the genomic DNA G+C content is 66.7-74.5 mol% [1,[3][4][5][6][7][8][9][10][11][12][13]. Tessaracoccus species have been retrieved from marine environments (marine sediment and sea sand) [6,8], soils (oil-contaminated soils and Antarctican soil) [3,4,13], industrial environments (drainage system of a lindane-producing factory, deep subsurface of the pyrite belt, and metal working fluid) [9][10][11], animals (rockfish and rhinoceros) [5,12], activated sludge biomass [1], and sewage treatment plants [7]. Insect guts harbour a large variety of microbes that affect host physiology including development, nutrition, metabolism, immunity, defence, behaviour, and detoxification [14][15][16][17][18].…”

“…Tessaracoccus species are characterized mainly as Gramstain-positive, non-motile, non-spore-forming, facultatively anaerobic bacteria that are catalase-positive and oxidasenegative. ʟʟ-Diaminopimelic acid is the diagnostic cell wall peptidoglycan amino acid, MK-9 or MK-10 is the major isoprenoid quinone, and anteiso-C 15 : 0 is the major fatty acid; the genomic DNA G+C content is 66.7-74.5 mol% [1, [3][4][5][6][7][8][9][10][11][12][13]. Tessaracoccus species have been retrieved from marine environments (marine sediment and sea sand) [6,8], soils (oil-contaminated soils and Antarctican soil) [3,4,13], industrial environments (drainage system of a lindane-producing factory, deep subsurface of the pyrite belt, and metal working fluid) [9][10][11], animals (rockfish and rhinoceros) [5,12], activated sludge biomass [1], and sewage treatment plants [7].…”