Tessaracoccus coleopterorum sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus

Abstract

A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus. The isolate was Gram-stain-positive, facultatively anaerobic, non-motile, coccus-shaped, and formed pale orange colonies. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %. The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0. The main respiratory quinone was MK-9 (H4). The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 69.0 %. The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan. The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus . We propose the name Tessaracoccus coleopterorum sp. nov. The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).

Brevilactibacter coleopterorum sp. nov., isolated from the intestine of the dark diving beetle Hydrophilus acuminatus, and Weissella coleopterorum sp. nov., isolated from the intestine of the diving beetle Cybister lewisianus

“…Suitable growth temperatures were determined by growing the isolates at 4,10,15,20,25,30,37,40,45, 55 and 65 °C using in trypticase soy broth (TSB; Bacto) for strain HDW11 T or Gifu anaerobic medium (GAM; Nissui) for strain HDW19 T . Growth at 0 °C was assessed by colony formation on TSA and GAM plates for strains HDW11 T and HDW19 T , respectively.…”

“…Genomic DNA of the isolates and W. diestrammenae KACC 16890 T , for which no genome sequence is currently available, were extracted using the MG Genomic DNA Purification kit (Macrogen). A 20 kb sequencing library was constructed, following the method of Hyun et al [30]. Then, 240 min movies were captured for each single-molecule real-time (SMRT) cell using the PacBio RS II (Pacific Biosciences) sequencing platform, based on C4 chemistry.…”

Nocardioides palaemonis sp. nov. and Tessaracoccus palaemonis sp. nov., isolated from the gastrointestinal tract of lake prawn

“…The sample was diluted to 10 −1 -, 10 −2 -and 10 −3 -fold (in order) using phosphate-buffered saline (Bioneer). The diluted samples of strains J2M5 T and J1M15 T were inoculated onto Reasoner's 2A (R2A; Difco) agar plates [18,33] and tryptic soy broth (TSB; Difco) agar (TSA) plates [27] and incubated under aerobic conditions at 30 °C for 1 week. The bacterial cells were subcultured thrice to obtain a pure culture.…”

“…The type species of the genus Tessaracoccus was first isolated from a biomass sample obtained from a laboratory-scale sequencing batch reactor [24]. Other species were isolated from various environments, ranging from soil and marine sediments to the intestines of insects, fish and even humans [25][26][27][28][29][30][31]. The members of Tessaracoccus are mostly Gram-stain-positive and facultatively aerobic, with rod-shaped and non-motile cells.…”

“…The major polar lipids are phosphatidylglycerol and diphosphatidylglycerol. These bacteria show G+C contents of roughly 61-74 % [24,27,28,32].…”