Tessaracoccus flavus sp. nov., isolated from the drainage system of a lindane-producing factory

Abstract

Strain RP1 T , a Gram-stain-positive, non-motile, non-spore-forming, coccus-shaped bacterium, was isolated from drainage of India Pesticides Limited, a lindane-producing unit situated at Chinhat, Lucknow, India. 16S rRNA gene sequence analysis revealed that strain RP1 T belongs to the family Propionibacteriaceae and was closely related to the members of the genus Tessaracoccus with a similarity range of 95.4-97.6 %. Strain RP1 T was facultatively anaerobic, oxidase-negative, catalase-positive and capable of nitrate reduction. Strain RP1 T contained peptidoglycan type A3c9, with LL-diaminopimelic acid as the diagnostic diamino acid and glycine at position 1 of the peptide subunit. The major cellular fatty acid of strain RP1 T was anteiso-C 15 : 0 but a significant amount of iso-C 14 : 0 was also detected. MK-9(H 4 ) was the major respiratory quinone and polyamines detected were spermine and spermidine. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and two unknown phospholipids. The G+C content of the DNA was 66.7 mol%. The levels of DNA-DNA relatedness between RP1 T and Tessaracoccus lubricantis KSS-17Se T , Tessaracoccus oleiagri SL014B-20A1 T and Tessaracoccus flavescens SST-39 T were 49.8, 34.8 and 23.5 %, respectively. Based on the phenotypic and phylogenetic data presented, strain RP1 T can be differentiated from previously described species of the genus Tessaracoccus, and thus represents a novel species, for which the name Tessaracoccus flavus sp. nov. is proposed. The type strain is RP1 T (5DSM 100159 T 5MCC 2769 T 5KCTC 39686 T ).The genus Tessaracoccus was first described by Maszenan et al. (1999) as the member of the family Propionibacteriaceae (Delwiche, 1957), the description of which has been emended twice (Stackebrandt et al., 1997;Zhi et al., 2009) The type strains of the genus Tessaracoccus are Gramstain-positive, facultatively anaerobic (except T. lubricantis), non-spore-forming, non-motile, oval, coccus-to rodshaped bacteria. The cell-wall peptidoglycan is of type A3c9, with LL-diaminopimelic acid (LL-Dpm) as the diagnostic diamino-acid. MK-9(H 4 ) is the major respiratory quinone and anteiso-C 15 : 0 is the predominant cellular fatty acid (Maszenan et al., 1999).We have already characterized several novel bacterial species from stressed environments including fly ash dumping sites of thermal power plants in Delhi and Kanpur, India (Prakash & Lal, 2006;Prakash et al., 2007), oil-contaminated site, Panipat oil refinery, India (Gupta et al., 2008(Gupta et al., , 2009, and a hexachlorocyclohexane dumpsite, situated at Ummari village, Lucknow, India (Kumar et al., 2008(Kumar et al., , 2015Singh & Lal, 2009;Verma et al., 2009Verma et al., , 2015Sharma et al., 2010;Malhotra et al., 2012;Dwivedi et al., 2013; Singh et al., 2013Singh et al., , 2014Singh et al., , 2015 Mahato et al., 2015). To further analyse the bacteria from the hexachlorocyclohexane dumpsite, samples were collected from the drainage system of a lindane-producing unit (Lucknow, Uttar ...

Tessaracoccus antarcticus sp. nov., a rhodopsin-containing bacterium from an Antarctic environment and emended description of the genus Tessaracoccus

“…The draft genome sequence of strain JDX10 T consisted of 13 contigs (>1000 bp) of 3, 649,759 bp with shortest contig size of 1011 bp and longest contig size of 1, 639, 251 bp. From the draft genome sequence, the G+C content of strain JDX10 T was calculated to be 66.9 mol%, which is in accordance with reported values for Tessaracoccus species (66.7-74.5 mol%) [1][2][3][4][5][6][7][8][9][10][11]. The related genome data for strain JDX10 T and closely related type strains are listed in Table S1, available in the online version of this article.…”

“…Moreover, the predominant cellular fatty acid component is anteiso-C 15 : 0 . Species of the genus Tessaracoccus have a high DNA G+C content, ranging from 66.7 to 74.5 mol% [1][2][3][4][5][6][7][8][9][10][11]. The increasing availability of molecular identification methods has provided microbiologists with ever-increasing opportunities to identify novel species, driving an important expansion in bacterial taxonomy.…”

Genome-Based Taxonomic Classification of the Phylum Actinobacteria

“…The description is as before (Kumari et al, 2016 ) with the following modification. The G+C content of the type-strain genome is 68.0%, its approximate size 3.19 Mbp, its GenBank deposit SAMN05428934 .…”

Complete Genome Sequence of Tessaracoccus sp. Strain T2.5-30 Isolated from 139.5 Meters Deep on the Subsurface of the Iberian Pyritic Belt

“…The genus contains nine validly published species, namely, T. bendigoensis (1), T. flavescens (2), T. lubricantis (3), T. lapidicaptus (4), T. oleiagri (5), T. rhinocerotis (6), T. flavus (7), T. massiliensis (8), T. defluvii (9), and one species, T. profundi (10), that has not been validly published. Tessaracoccus species have been isolated from diverse environments and are characterized as Gram-positive non-spore-forming facultative anaerobic bacteria (1).…”