Test for Escherichia coli Enterotoxin Using Infant Mice: Application in a Study of Diarrhea in Children in Honolulu

Dean, Andrew G.; Ching, Yi-Chuan; Williams, Ronald G.; Harden, Lewis B.

doi:10.1093/infdis/125.4.407

Cited by 828 publications

(331 citation statements)

References 9 publications

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“…Putative campylobacter colonies were identified by biochemical methods (Kaplan, 1980). The rest of the faecal sample was refrigerated and sent for identification of rotaviruses by electron microscopy (Rodriguez et al 1977) and eggs and cysts of parasites and protozoa by a concentration method (Faust, Russell & Jung, 1970 The same E. coli strains from each faecal culture were tested for production of heat-labile (LT) enterotoxin with a GM1-ELISA test (Svennerholm & Holmgren, 1978), using purified ganglioside kindly provided by Professor Lars Svennerholm, Institute of Neurochemistry, Gothenburg, Sweden, and for heat-stable (ST) enterotoxin with the infant mouse assay (Dean et al 1972).…”

Section: Diarrhoeal Disease In Mexican Childrenmentioning

confidence: 99%

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Cravioto¹,

Reyes²,

Ortega³

et al. 1988

Epidemiol. Infect.

137

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SUMMARYColonization of the intestine by putative pathogens was followed longitudinally in a cohort of 56 infants born during one calendar year in a rural Mexican village with faecal cultures taken every fortnight and every time a child had diarrhoea. The frequency of isolation of pathogens during episodes of diarrhoea was compared with that of matched controls from the same cohort. Incidence of diarrhoea during the first year of life was 98%, diminishing to 93% during the second year. The incidence curves for each year were not significantly different (P > 041). Isolation of enteropathogenic Escherichia coli, enterotoxigenic Escherichia coli producing heat-stable (ST) and/or heat-labile (LT) enterotoxins and rotaviruses was significantly higher in infants with diarrhoea during the first 2 years of life. In the case of shigella, although no significant differences were found by semester of life, 13 of 16 children in which these strains were found had diarrhoea. Isolation of Salmonella spp., Campylobacter spp. and protozoa were not significantly different in the two groups during the period studied. Strains showing localized adherence to HEp-2 cells or the presence of colonization factor antigens I or E8775 were found with significantly higher frequency in children with diarrhoea. Eighty-two percent of ST' or LT' ETEC strains isolated produced one of the three known colonization factors.

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Section: Diarrhoeal Disease In Mexican Childrenmentioning

confidence: 99%

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Cravioto¹,

Reyes²,

Ortega³

et al. 1988

Epidemiol. Infect.

137

View full text Add to dashboard Cite

show abstract

“…Specimens were streaked on to MacConkey agar and after overnight incubation, 10 lactose fermenting colonies, presumptive E. coli, were selectively picked and stored on nutrient (Columbia) agar slopes for immediate enterotoxin testing in pools and later biochemical identification and serotyping. Assay for heat stable toxin (ST) was carried out by the suckling mouse assay (Dean et al 1972). Mean gut: body weight ratios > 0-0700 were classified as positive.…”

Section: Methodsmentioning

confidence: 99%

Escherichia coliin gastroenteritis of children in Auckland, New Zealand

Goldwater¹,

Bettelheim²,

Rb³

1981

J. Hyg.

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SUMMARYA study of stool Escherichia coli in 60 children with gastroenteritis and 18 control children was carried out in Auckland, New Zealand in 1977. Toxigenic strains, heat labile and heat stable, predominated in the stools ofonly three children, all ofwhom had concomitant rotavirus. Classical enteropathogenic E. coli (EPEC) were found in patients and controls. Only one patient had many EPEC in the stool (086. H2), they were variably toxigenic and rotavirus was also present. No toxigenic serotype was isolated. Two potential pathogens were sometimes found. Overall there was no evidence for a substantial causative role for disease producing E. coli in these children.

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“…For the ST-like toxin assay, three mice of 3-5 days of age were used for each test [10]. The milk-filled stomach of each mouse was administered with 01 ml of the culture filtrate containing Evans blue (0-01 %).…”

Section: Toxin Assaymentioning

confidence: 99%

High prevalence of thermostable direct hemolysin (TDH)-like toxin inVibrio mimicusstrains isolated from diarrhoeal patients

et al. 1993

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SUMMARYA total of 17 isolates of Vibrio mimicus from patients, 29 from environment and 2 from food was examined for toxigenicity. Sixteen (94 %) clinical isolates and one (50%) from food produced TDH-like toxin, whereas none of the environmental isolates did so. The food from which V. mimicus with TDH-like toxin production was isolated, was one which had caused food poisoning. Only one environmental strain produced CT-like toxin, whilst ST-like toxin was not detected from any strains tested.

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Test for Escherichia coli Enterotoxin Using Infant Mice: Application in a Study of Diarrhea in Children in Honolulu

Cited by 828 publications

References 9 publications

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life

Escherichia coliin gastroenteritis of children in Auckland, New Zealand

High prevalence of thermostable direct hemolysin (TDH)-like toxin inVibrio mimicusstrains isolated from diarrhoeal patients

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