Testcross performance of doubled haploid lines from European flint maize landraces is promising for broadening the genetic base of elite germplasm

Brauner, Pedro Corrêa; Schipprack, Wolfgang; Utz, H. Friedrich; Bauer, Eva; Mayer, Manfred; Schön, Chris‐Carolin; Melchinger, Albrecht E.

doi:10.1007/s00122-019-03325-0

Cited by 28 publications

(22 citation statements)

References 47 publications

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“…S4). The yield gap between the mean testcross yield of the DH lines and the mean testcross yield of two commercial hybrids amounted to about 15% and was comparable to what was reported in the literature for other European landraces (Wilde et al 2010; Brauner et al 2019). The usefulness of the best 10% DH lines in KE and PE, respectively, remained 8% below the performance level of the commercial hybrids for TDMY (Fig.…”

Section: Discussionsupporting

confidence: 85%

European maize landraces made accessible for plant breeding and genome-based studies

et al. 2019

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Key messageDoubled-haploid libraries from landraces capture native genetic diversity for a multitude of quantitative traits and make it accessible for breeding and genome-based studies.AbstractMaize landraces comprise large allelic diversity. We created doubled-haploid (DH) libraries from three European flint maize landraces and characterized them with respect to their molecular diversity, population structure, trait means, variances, and trait correlations. In total, 899 DH lines were evaluated using high-quality genotypic and multi-environment phenotypic data from up to 11 environments. The DH lines covered 95% of the molecular variation present in 35 landraces of an earlier study and represent the original three landrace populations in an unbiased manner. A comprehensive analysis of the target trait plant development at early growth stages as well as other important agronomic traits revealed large genetic variation for line per se and testcross performance. The majority of the 378 DH lines evaluated as testcrosses outperformed the commercial hybrids for early development. For total biomass yield, we observed a yield gap of 15% between mean testcross yield of the commercial hybrids and mean testcross yield of the DH lines. The DH lines also exhibited genetic variation for undesirable traits like root lodging and tillering, but correlations with target traits early development and yield were low or nonsignificant. The presented diversity atlas is a valuable, publicly available resource for genome-based studies to identify novel trait variation and evaluate the prospects of genomic prediction in landrace-derived material.Electronic supplementary materialThe online version of this article (10.1007/s00122-019-03428-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 85%

European maize landraces made accessible for plant breeding and genome-based studies

et al. 2019

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“…Deleterious alleles from such germplasm are readily expressed in the haploid stage and can then be purged through natural or artificial selection. Hence, DH technology is a highly effective tool to access the genetic diversity present in allogamous landraces and to expand the genetic diversity of elite germplasm (Wilde et al 2010; Strigens et al 2013; Böhm et al 2017; Brauner et al 2019; Hölker et al 2019). The DH lines from landraces and OPVs can be evaluated in replicated trials with high precision, which is not possible when using landraces and OPVs due to their high heterogeneity (Strigens et al 2013).…”

Section: Advantages Of Dh Lines In Maize Breedingmentioning

confidence: 99%

Doubled haploid technology for line development in maize: technical advances and prospects

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Key Message Increased efficiencies achieved in different steps of DH line production offer greater benefits to maize breeding programs. Abstract Doubled haploid (DH) technology has become an integral part of many commercial maize breeding programs as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide. The established method for maize DH production covered in this review involves in vivo induction of maternal haploids by a male haploid inducer genotype, identification of haploids from diploids at the seed or seedling stage, chromosome doubling of haploid (D 0) seedlings and finally, selfing of fertile D 0 plants. Development of haploid inducers with high haploid induction rates and adaptation to different target environments have facilitated increased adoption of DH technology in the tropics. New marker systems for haploid identification, such as the red root marker and high oil marker, are being increasingly integrated into new haploid inducers and have the potential to make DH technology accessible in germplasm such as some Flint, landrace, or tropical material, where the standard R1-nj marker is inhibited. Automation holds great promise to further reduce the cost and time in haploid identification. Increasing success rates in chromosome doubling protocols and/or reducing environmental and human toxicity of chromosome doubling protocols, including research on genetic improvement in spontaneous chromosome doubling, have the potential to greatly reduce the production costs per DH line.

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“…European flint landraces are natural candidates for introgression into the flint heterotic pool, because this traces back to lines extracted from a small number of European flint landraces at the beginning of hybrid breeding (Reif et al, 2005;Wilde et al, 2010;Gouesnard et al, 2017;Brauner et al, 2019). For this reason, information about combining ability of the flint landraces in testcrosses with two representative elite tester lines from the dent heterotic pool is important.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it is very important to provide information about heterotic pattern before including gene banks' material into commercial breeding program. For this reason, many studies focused on heterotic grouping of landraces and identification of promising heterotic patterns among them (Revilla et al, 2006;Brauner et al, 2019). Vančetović et al (2015 b) pointed out that the value of initial population for breeding, if F1 hybrid is the main goal, besides characteristics per se, depends on its combining abilities in crosses with another population, group of populations or inbred line.…”

Section: Selection Of Plant Materialmentioning

confidence: 99%

Breeding potential of maize landraces evaluated by their testcross performance

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Kravić

Babić

et al. 2020

Zemdirbyste-Agriculture

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Significant amounts of crop diversity, especially in out-crossing species like maize (Zea mays L.) is, are often distributed both between and within related groups of accessions. Thus, a detailed characterization and classification of gene bank accessions should be performed prior to use of the best of them for introgression programs to enlarge the genetic base of the elite germplasm pool. Based on performances per se, 40 landraces were chosen out of the wide pool of Western Balkan landraces, previously clustered into 11 homogenous groups. The objective of this study was to evaluate the heterotic pattern of landraces in testcrossing with three divergent elite testers L217, L73B013 and L255/75-5. According to the results of a two-year trial conducted at four locations,

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Testcross performance of doubled haploid lines from European flint maize landraces is promising for broadening the genetic base of elite germplasm

Cited by 28 publications

References 47 publications

European maize landraces made accessible for plant breeding and genome-based studies

European maize landraces made accessible for plant breeding and genome-based studies

Doubled haploid technology for line development in maize: technical advances and prospects

Breeding potential of maize landraces evaluated by their testcross performance

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