Testicular Activity of Mice Treated With MeOH Extract of Achyranthes aspera Leaves

Abstract

Objectives:In India control of fertility is based on the folk use of numerous Indian Traditional Plants that has been practiced for contraception from many years. Present investigation aims that, to evaluate the contraceptive potential of Achyranthes aspera leaves MeOH extract in male albino mice and has shown promising results of antispermatogenic, antiandrogenic and hypolipidaemic activities. Materials & Methods:MeOH extract of A. aspera leaves at the doses of 25 mg and 50 mg/100 gm body weight was administered orally for 30 days to adult male albino mice. On day 31st, the rats were sacrificed and the testis were removed and weighed for gravimetrical analysis. Organs were processed for biochemical estimation, histological evaluation and histomorphometry for testicular analysis.Results: Treatment with A. aspera leaves extract resulted in decrease the weight of testis 0.925±0.015 significantly (P<0.05). Morphometric analysis like diameter of testis 2554.22±18.545 seminiferous tubules 247.074±8.051 and Leydig cells 7.842±0.248 were decreased significantly (P<0.05) at 50mg/100gm body weight when compare to control. Spermatogenic elements like, spermatogonia, spermatocytes and spermatids in the testis were reduced significantly (P<0.05) as well as Leydig cells count in testis when compared to control. Biochemical analysis resulted as, protein, glycogen, and cholesterol content were decreased significantly (P<0.05) in testis of extracts treated mice, whereas alkaline phosphatase increased and acid phosphatase decreased when compared with that of control. Conclusion:MeOH extract of A. aspera leaves arrest the spermatogenesis and inhibited testicular function in male mice without side effects to act as potential contraceptive.

Androgenic and Spermatogenic Effects of Methanolic Root Extract of Waltheria indica (linn) in Male Wistar Rats

“…39 Alkaline phosphatase (ALP) and acid phosphatase (ACP) are widely distributed in the testis and both enzymes have been involved in the intercellular and intracellular transportation of metabolites during steroidogenesis and release of gonadotropin. 40,41 ACP facilitates the exchange of materials between the germinal and Sertoli cells and enhances the maturation of spermatocytes during spermatogenesis. 42 ALP is involved in mobilizing carbohydrates and lipid metabolites to be utilized by accessory sex structure or by the sperm cells in the seminal fluid.…”

Review on effects of obesity on male reproductive system and the role of natural products

“…Many natural products have also shown their potential beneficial effects in treating post-testicular impairment in male obesity as shown in Tables 1 and 2. A study carried out on HFDinduced obese mice for 30 days shows that Achyranthes bidentata Blume (Kamble et al, 2017) decreases spermatogenesis and inhibits testicular function without any side effects suggesting its potential contraceptive property (Rani et al, 2012). Another study carried out by Wu et al (2009) on glucose challenged db/ db mice treated with Acorus calamus Linn.…”

Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

“…[25] The pharmacomedical effects of this plant is believed to be due to the present of certain phytochemicals such as: saponins, [26] β-D galactopyranosyl ester of D-Glucuronic Acid (also known as saponins B) and D-Glucuronic Acid (saponins A). [27] In male reproductive medicine model, A. aspera roots have been reported to possess spermicidal activity in both human and rat [28] and administration of A. aspera leaves extracts causes reduction in spermatogenesis, steroidogenesis, androgen production, and alteration of sexual behavior in Swiss albino mice. [29] Albino mice are one of the two white-colored varieties of the domesticated house mouse, Mus musculus, and according to scientific American report, Albinism in mice is the result of a single genetic color factor being lost, which leads to a loss of pigmentation in the skin and eyes.…”

“…Herbal drugs constitute a major part of many traditional medical systems globally [7] and Plants (including A. aspera) can still serve as possible sources for new drugs and therapeutic chemicals [28] but literature on the effect of A. aspera extract on hematological parameters of mammals is scanty, and effect of this plant's leaf extract on the hematological parameter of albino mice in Northeastern Nigeria have not yet been fully studied therefore, this study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on the hematological parameter of mice in other to elucidate the patho-hematological effects and hematotoxicity of this plant in traditional medicinal care. It is believed that the information obtained in this study will enhance safe and appropriate use of Achyranthes aspera in folkloric medicine in Nigeria.…”