Testicular cell cytoskeleton in the newt,Triturus marmoratus marmoratus, during the annual cycle

Ramos, Purificación; Miguel, María P. De; Arenas, María Isabel; Fraile, Benito; Paniagua, Ricardo

doi:10.1002/(sici)1097-0029(19960415)33:6<501::aid-jemt5>3.0.co;2-n

Cited by 2 publications

(1 citation statement)

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“…Immunohistochemical studies related to IF desmin reveal its presence at varying intensities in the region of the testicular albuginea in the analysed lizards, indicating the probable existence of contractile cells in this connective tissue layer. A similar result was also observed in humans, rats and Korean water deer and Reeves' muntjac testis (Paranko & Pelliniemi, 1992;Sohn et al, 2013;Virtanen et al, 1986); in quails, domestic roosters, turkeys and ducks (Aire & Ozegbe, 2007), and in fish, where the peritubular cells were desmin-positive (Arenas et al, 1995;Ramos et al, 1996).…”

Section: Discussionsupporting

confidence: 78%

Histological study and immunohistochemical location of cytoskeletal proteins in the testis and epididymis of the three species of lizards of the family Leiosauridae (Reptilia: Squamata)

et al. 2022

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The objective of the study was to histologically describe the structure of the testis and epididymis, identifying the GAGs in them. Furthermore, the distribution of smooth muscle alpha‐actin, desmin, and vimentin is described of E. bilineatus, E. perditus, and U. vautieri, through immunohistochemical method. Two adult males of each species of the lizards were collected in southeastern Brazil. In the testicular albuginea, the presence of neutral glycoproteins was detected. In E. bilineatus, the epididymal duct epithelium is simple, composed of secretory cells, cylindrical in shape and acidophilic cytoplasm. In the testis of the three lizards there was a positive immunolocation accentuated for α‐SMA in the dense connective tissue of the tunica albuginea. A moderate reaction was observed in the connective tissue albuginous and in the layer of smooth muscle fibers in a circumferential arrangement of the epididymal duct. The immunolocation for desmin was accentuated in the fibers of the testicular albuginea of E. bilineatus, while in U. vautier and E. perditus was moderate. In the epididymis, only in E. perditus and E. bilineatus positive immunoreaction was verified. In the three lizards there were no observations of immunostaining for vimentin with the antibody utilized.

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