Testicular dysfunction induced by aluminum oxide nanoparticle administration in albino rats and the possible protective role of the pumpkin seed oil

Abstract

Background Even though the widespread of nanoalumina and their benefits in all fields, its potential impacts on male reproductive system have limited information. Objective The present study was conducted to investigate the testicular dysfunction of nanoalumina and the protective role of pumpkin seed oil (PSO) against potential adverse impacts induced by alumina nanoparticles (Al2O3-NPs) in male rat. Methodology Al2O3-NPs were administered to the rat orally at a dose of 70 mg/kg body weight once a day for 28 successive days, while pumpkin seed oil was administered to the rat orally at 4 mL/kg b w before administration of Al2O3-NPs, once a day for 28 successive days. After the administration period, sperm concentration, motility, morphology, and DNA damage, as biomarkers of reproductive toxic effects, were evaluated using sperm analysis and comet assays, and histopathological examination of testis was performed. In addition, level of the serum testosterone hormones were estimated, and the levels of oxidative stress biomarkers that take part in the reproductive pathologies such as catalase, glutathione, and malondialdehyde were estimated. Results The present results revealed that Al2O3-NPs induced DNA damage in testicular cells, marked histopathological alterations, and caused a significant elevation in MDA in testicular tissue. There was a significant decline in GSH and CAT activities. Furthermore, there was a significant decline in serum testosterone level in the testicular tissue of Al2O3-NP-administered rats. In contrast, pumpkin seed oil co-administration alleviated DNA damage and improved the histopathological alterations in the testicular tissues. Moreover, pumpkin seed oil co-administration significantly reduced MDA and improved the antioxidant defenses in testicular tissue. Conclusion The current study concluded that Al2O3-NPs caused testicular dysfunction by generating oxidative injury. Otherwise, PSO co-administration successfully attenuated the adverse impacts of Al2O3-NPs via suppression of oxidative stress and apoptosis as well as enhancement of the antioxidant defense system.

Effect of dietary pumpkin (Cucurbita moschata) seed oil supplementation on reproductive performance and serum antioxidant capacity in male and nulliparous female V-Line rabbits

“…Therefore, dietary supplementation with neutraceutical possessing antioxidant properties is crucial to improve the oxidative status and enhance fertility. PS oil was formerly used as a dietary antioxidant to improve male fertility in rats with testicular dysfunction due to oxidative damage induced by aluminium oxide (Hamdi 2020). Additionally, PS oil in combination with vitamin E alleviated testicular injury in the rat (Hashemi 2013).…”

Potential Toxic Effects of Aluminum Nanoparticles: An overview

“…Moreover, CAT and SOD activities were significantly altered (De et al, 2020). Administration of AlNPs to rats orally at the dose of 70 mg/kg daily for 28 days resulted in elevated testicular MDA level and decreased GSH level and CAT activity (Hamdi, 2020). Exposure of male mice to AlNPs (50 and 13 nm) through a nasal drip three times a day at a dose of 50 mg/kg resulted in increased aluminum accumulation in the spleen, with the 13 nm AlNP group having the highest accumulations.…”

Elevated IgG Antibody to Aluminum Bound to Human Serum Albumin in Patients with Crohn’s, Celiac and Alzheimer’s Disease

“…Aluminum has been found to adversely affect the gastrointestinal system, the reproductive system, and particularly the nervous system [ 8 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. However, the impact of aluminum in the gut was not known until the 2013 study by de Chambrun et al [ 15 ].…”

“…The neurotoxicity of aluminum has been demonstrated in tissue culture, animal models, and its link with diseases such as encephalopathy, Parkinson’s disease (PD) and Alzheimer’s disease (AD), and chromic renal failure [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. In relation to the neurotoxicity of the metal in AD, mounting evidence has suggested the induction of β-amyloid oligomerization by aluminum action as a cross-linker and the integration of aluminum into the amyloid cascade [ 8 ].…”