Testicular hyperthermia reduces testosterone concentrations and alters gene expression in testes of Nelore bulls

Rizzoto, Guilherme; Ferreira, João Carlos Pinheiro; Codognoto, Viviane Maria; Oliveira, Kellen; García, Henry David Mogollón; Pupulim, A. G.; Neto, Francisco José Teixeira; Castilho, A. C. S.; Nunes, Sarah Gomes; Thundathil, Jacob C.; Kastelic, John P.

doi:10.1016/j.theriogenology.2020.04.029

Cited by 19 publications

(2 citation statements)

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“…Downregulation of gene expression was accompanied by a decrease in the diameter, length and volumetric proportion of seminiferous cords in male foetuses from cows fed an ad libitum diet. Rizzoto et al (2020) also observed a profound reduction in testicular testosterone concentrations by heat stress‐induced downregulation of the STAR gene in Nellore bulls. On the other hand, an increase in testicular growth rate in young bulls was associated with the observation of high levels of this protein in bulls treated with Deslorelin 1 (Aspden et al, 1998).…”

Section: Discussionmentioning

confidence: 94%

“…The only candidate gene prioritized by ToppGene analysis, STAR (position 33.312–33.320 Mb on BTA27), is one of the most important factors associated with the steroidogenic pathway, including testosterone production in Leydig cells (Brown & Goldstein, 1997; Eacker et al, 2008; Rizzoto et al, 2020). The protein encoded by the STAR gene plays a key role in the acute regulation of steroid hormone synthesis, increasing the conversion/cleavage of cholesterol to pregnenolone mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane (Stelzer et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Testicular hypoplasia in Nellore Cattle: Genetic analysis and functional analysis of genome‐wide association study results

Silva

Gondro

Fonseca

et al. 2022

J Animal Breeding Genetics

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Characterized by the incomplete development of the germinal epithelium of the seminiferous tubules, Testicular hypoplasia (TH) leads to decreased sperm concentration, increased morphological changes in sperm and azoospermia.Economic losses resulting from the disposal of affected bulls reduce the efficiency of meat production systems. A genome-wide association study and functional analysis were performed to identify genomic windows and the underlying positional candidate genes associated with TH in Nellore cattle. Phenotypic and pedigree data from 207,195 animals and genotypes (461,057 single nucleotide polymorphism, SNP) from 17,326 sires were used in this study. TH was evaluated as a binary trait measured at 18 months of age. A possible correlated response on TH resulting from the selection for scrotal circumference was evaluated by using a two-trait analysis. Thus, estimated breeding values were calculated by fitting a linear-threshold animal model in a Bayesian approach. The SNP effects were estimated using the weighted single-step genomic BLUP method. Twelve nonoverlapping windows of 20 adjacent SNP that explained more than 1% of the additive genetic variance were selected for candidate gene annotation. Functional and gene prioritization analysis of the candidate genes identified genes (KHDRBS3, GPX5, STAR, ERLIN2), which might play an important role in the expression of TH due to their known roles in the spermatogenesis process, synthesis of steroids and lipid metabolism.

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Section: Discussionmentioning

confidence: 94%