Testicular postgenomics: targeting the regulation of spermatogenesis

Rolland, Antoine; Jégou, Bernard; Pineau, Charles

doi:10.1098/rstb.2009.0294

Cited by 40 publications

(32 citation statements)

References 142 publications

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“…However, the biogenic mechanisms and function of these ncRNAs remain unknown. Previous studies have suggested that the ncRNAs in testis are involved mainly in gene regulation at the posttranscriptional and translational levels during spermatogenesis (34,35). The activities of several autosomal ncRNAs have been associated with the initiation and maintenance of meiotic sex chromosome inactivation, an important gene-silencing process that is established at midpachytene stage and persists to the postmeiotic phase of spermatogenesis (36).…”

Section: Discussionmentioning

confidence: 99%

Male-specific region of the bovine Y chromosome is gene rich with a high transcriptomic activity in testis development

Chang

Yang²,

Retzel

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

118

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The male-specific region of the mammalian Y chromosome (MSY) contains clusters of genes essential for male reproduction. The highly repetitive and degenerative nature of the Y chromosome impedes genomic and transcriptomic characterization. Although the Y chromosome sequence is available for the human, chimpanzee, and macaque, little is known about the annotation and transcriptome of nonprimate MSY. Here, we investigated the transcriptome of the MSY in cattle by direct testis cDNA selection and RNA-seq approaches. The bovine MSY differs radically from the primate Y chromosomes with respect to its structure, gene content, and density. Among the 28 protein-coding genes/families identified on the bovine MSY (12 single- and 16 multicopy genes), 16 are bovid specific. The 1,274 genes identified in this study made the bovine MSY gene density the highest in the genome; in comparison, primate MSYs have only 31–78 genes. Our results, along with the highly transcriptional activities observed from these Y-chromosome genes and 375 additional noncoding RNAs, challenge the widely accepted hypothesis that the MSY is gene poor and transcriptionally inert. The bovine MSY genes are predominantly expressed and are differentially regulated during the testicular development. Synonymous substitution rate analyses of the multicopy MSY genes indicated that two major periods of expansion occurred during the Miocene and Pliocene, contributing to the adaptive radiation of bovids. The massive amplification and vigorous transcription suggest that the MSY serves as a genomic niche regulating male reproduction during bovid expansion.

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Section: Discussionmentioning

confidence: 99%

Male-specific region of the bovine Y chromosome is gene rich with a high transcriptomic activity in testis development

Chang

Yang²,

Retzel

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

118

View full text Add to dashboard Cite

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“…The present review focuses on spermatogenesis-related proteomics in our laboratory and complements other reviews. [16][17][18][19][20] This review aims to improve our understanding of normal spermatogenesis and the cause of abnormalities in spermatogenesis and offers an interpretation of the regulatory mechanisms underlying male fertility and infertility.…”

Section: Introductionmentioning

confidence: 99%

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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“…These include the use of proteomics and genomics (Calvel et al 2010) and mouse genetic models (Verhoeven et al 2010) to As depicted in figure 1c which summarizes different cellular events that occur during spermatogenesis, it is obvious that some of the events listed on the right panel, such as spermiogenesis and its related cellular processes (e.g. spermatid adhesion to Sertoli cells) can become prime targets to disrupt spermatogenesis, leading to male infertility.…”

Section: Spermatogenesis: the Presentmentioning

confidence: 99%

The biology of spermatogenesis: the past, present and future

Cheng

Mruk

2010

Phil. Trans. R. Soc. B

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The physiological function of spermatogenesis in Caenorhabditis elegans, Drosophila melanogaster and mammals is to produce spermatozoa (1n, haploid) that contain only half of the genetic material of spermatogonia (2n, diploid). This half number of chromosomes from a spermatozoon will then be reconstituted to become a diploid cell upon fertilization with an egg, which is also haploid. Thus, genetic information from two parental individuals can be passed onto their offspring. Spermatogenesis takes place in the seminiferous epithelium of the seminiferous tubule, the functional unit of the mammalian testis. In mammals, particularly in rodents, the fascinating morphological changes that occur during spermatogenesis involving cellular differentiation and transformation, mitosis, meiosis, germ cell movement, spermiogenesis and spermiation have been well documented from the 1950s through the 1980s. During this time, however, the regulation of, as well as the biochemical and molecular mechanisms underlying these diverse cellular events occurring throughout spermatogenesis, have remained largely unexplored. In the past two decades, important advancements have been made using new biochemical, cell and molecular biology techniques to understand how different genes, proteins and signalling pathways regulate various aspects of spermatogenesis. These include studies on the differentiation of spermatogonia from gonocytes; regulation of spermatogonial stem cells; regulation of spermatogonial mitosis; regulation of meiosis, spermiogenesis and spermiation; role of hormones (e.g. oestrogens, androgens) in spermatogenesis; transcriptional regulation of spermatogenesis; regulation of apoptosis; cell -cell interactions; and the biology of junction dynamics during spermatogenesis. The impact of environmental toxicants on spermatogenesis has also become an urgent issue in the field in light of declining fertility levels in males. Many of these studies have helped investigators to understand important similarities, differences and evolutionary relationships between C. elegans, D. melanogaster and mammals relating to spermatogenesis. In this Special Issue of the Philosophical Transactions of the Royal Society B: Biological Sciences, we have covered many of these areas, and in this Introduction, we highlight the topic of spermatogenesis by examining its past, present and future.

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Testicular postgenomics: targeting the regulation of spermatogenesis

Cited by 40 publications

References 142 publications

Male-specific region of the bovine Y chromosome is gene rich with a high transcriptomic activity in testis development

Male-specific region of the bovine Y chromosome is gene rich with a high transcriptomic activity in testis development

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

The biology of spermatogenesis: the past, present and future

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