Testing for Neisseria gonorrhoeae by nucleic acid amplification testing of chlamydia samples using Roche Cobas Amplicor in a rural area in the north of England does not find more gonorrhoea in primary care

Stanley, B

doi:10.1136/sti.2004.013722

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…5 In particular, concerns that the ratio of false-positives to true positives could be high in areas of low prevalence. 6,7 The APTIMA Combo 2 (AC2) assay combines transcription-mediated amplification (TMA) with magnetic target capture of rRNA to detect both NG and CT in the same tube. With this method specimen collection kits are provided that can be transported to the Laboratory at ambient temperatures for endocervical and vulvovaginal swabs or stabilized urine.…”

Section: Introductionmentioning

confidence: 99%

The contribution of APTIMA Combo 2 assay to the diagnosis of gonorrhoea in genitourinary medicine setting

Moss

Mallinson²

2007

Int J STD AIDS

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For 929 female and 821 male patients attending a genitourinary clinic, samples intended for chlamydia diagnosis were dual tested by nucleic acid amplification for both chlamydia and Neisseria gonorrhoeae (NG). The assay used, Gen-probe APTIMA Combo 2 (AC2) detected all cases of NG found by conventional microscopy and culture. AC2 identified additional patients who had partners with NG, but were themselves negative by microscopy and culture. Few, if any, false-positive AC2 results were found. Use of AC2 increased the number of patients treated for NG. It can reduce the number of specimens required and may limit the need for multiple site testing.

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Section: Introductionmentioning

confidence: 99%

The contribution of APTIMA Combo 2 assay to the diagnosis of gonorrhoea in genitourinary medicine setting

Moss

Mallinson²

2007

Int J STD AIDS

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“…Although a screening programme for gonococcal infection in conjunction with NCSP is likely to meet most of the criteria set by the national screening committee, its cost effectiveness will depend on the prevalence of gonococcal infection in the local population 16 – 18…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Neisseria gonorrhoeae infection in young subjects attending community clinics in South London

Rao

Bacon²,

Evans³

et al. 2008

Sexually Transmitted Infections

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“…1,2 However, there are concerns about specificity of NAATs in low-prevalence NG populations and its associated false positivity (FP), impacting on positive predictive values. 3–5 FP can occur with some NAATs as their target may cross-react with different species of Neisseria. 2,6 The evidence may be less for Aptima Combo 2 (AC2, Gen-Probe, San Diego, CA, USA), but to date this type of FP has not been observed with AC2 or Aptima Gonococcus (AGC) assays.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis, management and prevalence estimation of gonorrhoea: influences of Aptima Combo 2 assay with alternative target confirmation

et al. 2009

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Case-notes and laboratory data were retrospectively reviewed for influences of dual testing by Aptima Combo 2 (AC2) for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) on the diagnosis, management and prevalence estimation of gonorrhoea in the genitourinary (GU) medicine clinic and community. NG positives by AC2 were confirmed by Aptima Gonococcus assay. Unconfirmed positives were rare. Our study showed that in the GU medicine clinic, AC2 detected about 20% extra cases of NG beyond culture. For best standard of care, NG culture and microscopy are still required in some patients to ensure that treatment is rapid and appropriate. Compared to self-referral at the GU medicine clinic, community tests made a substantial contribution to the overall number of NG cases found (40 community versus 35 Macclesfield GU medicine clinic). The ratio of female to male NG cases found was significantly higher (P = 0.002) in the community (13 M, 27 F) than at the GU medicine clinic (25 M, 10 F). In the community, over 60% of NG infections occurred in chlamydia-negative patients. The overall prevalence of NG in the GU medicine clinic was 1.3%, the true prevalence being much lower at 0.9% on primary test. Prevalence in the community was 0.4%. Data from dual testing in the community can clarify NG prevalence beyond the existing KC60 (sexually transmitted infections) reports.

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Testing for Neisseria gonorrhoeae by nucleic acid amplification testing of chlamydia samples using Roche Cobas Amplicor in a rural area in the north of England does not find more gonorrhoea in primary care

Cited by 5 publications

References 5 publications

The contribution of APTIMA Combo 2 assay to the diagnosis of gonorrhoea in genitourinary medicine setting

The contribution of APTIMA Combo 2 assay to the diagnosis of gonorrhoea in genitourinary medicine setting

Prevalence of Neisseria gonorrhoeae infection in young subjects attending community clinics in South London

Diagnosis, management and prevalence estimation of gonorrhoea: influences of Aptima Combo 2 assay with alternative target confirmation

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