Testing of microsatellite primers with different populations of Eurasian spruces Picea abies (L.) Karst. and Picea obovata Ledeb.

Abstract

From a clone library containing microsatellite DNA fragments of Norway spruce, seven pairs of primers were selected. These primers were tested as markers in the genetic structure analysis of nine popula tions of Eurasian spruce species Picea abies (L.) Karst. and Picea obovata Ledeb. Five pairs of these primers identified polymorphic loci with allele numbers ranging from 6 to 15. In the populations examined, the observed and expected heterozygosity values assessed at five loci varied from 0.1778 to 0.6556 and from 0.7800 to 0.900, respectively. In the populations examined, the values of F st index varied from 0.0691 to 0.2551 with the mean value of 0.1318. On the dendrogram based on Nei genetic distances, the populations formed three groups: Pskov-Ciscarpathya, Komi-Tatarstan-Arkhangelsk, Kazakhstan-Karelia(natural)-Karelia(plan tation)-Krasnoyarsk. Five of the primer pairs tested proved to be useful for analysis of the population genetic structure in Eurasian spruce species.

Use of Nuclear Microsatellite Loci for Evaluating Genetic Diversity of Selected Populations of Picea abies (L.) Karsten in the Czech Republic

“…Occurrences of null alleles were seen especially in loci PAAC19 and SpAGD1. A similar failure of amplifications for locus PAAC19 has been observed by Rungis et al [24] and for locus SpAGD1 by Melnikova et al [23]. Due to their recessive behavior, null alleles cause a shortage of heterozygote genotypes and result in incorrect estimates of allele frequencies.…”

“…SSR markers were used as molecular genetic markers for detecting genetic variation in the selected Norway spruce units. The following markers with dinucleotide motifs, whose PCR products provided single, clear, reproducible patterns were used: PAAC23, PAAC19 [22], WS00716.F13, WS0092.A19, WS0022.B15, WS0073.H08, WS00111.K13, WS0023.B03 [24] derived from expressed sequence tags (EST-SSRs), and SpAGD 1 [23] developed from genomic libraries of Norway spruce [21]. The nine nuclear microsatellite markers were assembled into three multiplexes from the viewpoint of the targeted allele sizes and amplification conditions.…”

“…Knowledge based on DNA analyses regarding the variability of genetic resources will contribute to the quality of the reproduction material and to creating an optimal species composition in forests. Norway spruce has been the subject of numerous genetic surveys using isozymes [10,11], expressed sequence tags markers [12,13], mitochondrial DNA [14,15], sequence tagged site markers [16,17], amplified fragment length polymorphism [18], single nucleotide polymorphisms [19,20], and microsatellite markers [21][22][23][24][25]. Nuclear simple sequence repeats (SSRs, or microsatellites) are widely used for assessing genetic diversity in forestry populations [6].…”

Assessment of phenotypic plasticity of spruce species Picea abies (L.) Karst. and P. obovata (Ledeb.) on provenances tests in European North of Russia

“…they are not plastic in all studied parameters (they can be named as partly plastic). Such genomic clustering of parent populations is typical of Eurasian spruce species (Melnikova et al 2012). According to Garzón et al (2011), different response to climatic changes can be expected for species and provenances with different phenotypic plasticity.…”