Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

Milatović, D.; Nikolić, D.; Krška, B.

doi:10.17221/219/2012-hortsci

Cited by 13 publications

(9 citation statements)

References 28 publications

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“…A wide apricot germplasm was used for assay testing and validation, with the aim of including a wide genetic variability. Results were largely in agreement with genotypes/phenotypes reported in the literature, although some inconsistencies were observed ( Table 1 ): in the case of ‘Harleyne’, characterized by the S 3 /S 20 S-RNAase allele, the presence of the heterozygous SFB C allele is congruent with its well-known SC phenotype [ 35 ], suggesting the hypothesis of a linkage breaking between SFB C and S C in the S -locus haplotype of this accession; in ‘Dorada’, ‘Ladycot’ and ‘Mirlo Naranja’, a heterozygous SFB C allele was detected in contrast to the homozygous S C /S C genotype reported in the literature, probably due to problems with the amplification of the incompatible(s) S-RNAase allele(s) [ 14 ]; in ‘Pricia’, an SC cultivar heterozygous for the S C allele, the SFB C allele was not detected although these cultivars were heterozygous for m -HET genotype; in this case, an erroneous attribution of the cultivars or a sampling error could provide a possible explanation.…”

Section: Discussionsupporting

confidence: 82%

Development of an HRMA-Based Marker Assisted Selection (MAS) Approach for Cost-Effective Genotyping of S and M Loci Controlling Self-Compatibility in Apricot (Prunus armeniaca L.)

Marchesano

Chiozzotto

Baccichet

et al. 2022

Genes

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The apricot species is characterized by a gametophytic self-incompatibility (GSI) system. While GSI is one of the most efficient mechanisms to prevent self-fertilization and increase genetic variability, it represents a limiting factor for fruit production in the orchards. Compatibility among apricot cultivars was usually assessed by either field pollination experiments or by histochemical evaluation of in vitro pollen tube growth. In apricots, self-compatibility is controlled by two unlinked loci, S and M, and associated to transposable element insertion within the coding sequence of SFB and ParM-7 genes, respectively. Self-compatibility has become a primary breeding goal in apricot breeding programmes, stimulating the development of a rapid and cost-effective marker assisted selection (MAS) approach to accelerate screening of self-compatible genotypes. In this work, we demonstrated the feasibility of a novel High Resolution Melting Analysis (HRMA) approach for the massive screening of self-compatible and self-incompatible genotypes for both S and M loci. The different genotypes were unambiguously recognized by HRMA, showing clearly distinguishable melting profiles. The assay was developed and tested in a panel of accessions and breeding selections with known self-compatibility reaction, demonstrating the potential usefulness of this approach to optimize and accelerate apricot breeding programmes.

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Section: Discussionsupporting

confidence: 82%

Development of an HRMA-Based Marker Assisted Selection (MAS) Approach for Cost-Effective Genotyping of S and M Loci Controlling Self-Compatibility in Apricot (Prunus armeniaca L.)

Marchesano

Chiozzotto

Baccichet

et al. 2022

Genes

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“…The self-(in)compatibility of 68 cultivars is reported herein for the first time. The results in the remaining 23 cultivars have been compared with previous reports in which self-(in)compatibility was determined by the evaluation of the percentage of fruit set after self-pollinations in the field (Egea and Burgos, 1996 ; Rodrigo and Herrero, 1996 ; Burgos et al, 1997 ; Egea et al, 2010 ; Muñoz-Sanz et al, 2017 ) or by the observation of pollen tube growth in pistils after self- and cross-pollinations (Egea and Burgos, 1996 ; Rodrigo and Herrero, 1996 ; Egea et al, 2010 ; Milatovic et al, 2013a , b ). Thus, results herein agree with previous reports for “Canino,” “Corbato,” “Luizet,” “Mirlo Blanco,” “Mirlo Anaranjado,” “Mitger,” “Palsteyn,” “Paviot,” “Pepito del Rubio,” “Tadeo,” and “Tom Cot” as self-compatible, and also for “Bergarouge,” “Goldrich,” “Goldstrike,” “Harcot,” “Hargrand,” “Moniqui,” “Orangered,” “Pinkcot,” “Robada,” “Stark Early Orange,” “Stella,” “Sun Glo,” and “Veecot” as self-incompatible.…”

Section: Discussionmentioning

confidence: 99%

Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele Identification, and Incompatibility Group Assignment

et al. 2018

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Apricot (Prunus armeniaca L.) is a species of the Rosaceae that was originated in Central Asia, from where it entered Europe through Armenia. The release of an increasing number of new cultivars from different breeding programs is resulting in an important renewal of plant material worldwide. Although most traditional apricot cultivars in Europe are self-compatible, the use of self-incompatible cultivars as parental genotypes for breeding purposes is leading to the introduction of a number of new cultivars that behave as self-incompatible. As a consequence, there is an increasing need to interplant those new cultivars with cross-compatible cultivars to ensure fruit set in commercial orchards. However, the pollination requirements of many of these new cultivars are unknown. In this work, we analyze the pollination requirements of a group of 92 apricot cultivars, including traditional and newly-released cultivars from different breeding programs and countries. Self-compatibility was established by the observation of pollen tube behavior in self-pollinated flowers under the microscope. Incompatibility relationships between cultivars were established by the identification of S-alleles by PCR analysis. The self-(in)compatibility of 68 cultivars and the S-RNase genotype of 74 cultivars are reported herein for the first time. Approximately half of the cultivars (47) behaved as self-compatible and the other 45 as self-incompatible. Identification of S-alleles in self-incompatible cultivars allowed allocating them in 11 incompatibility groups, six of them reported here for the first time. The determination of pollination requirements and the incompatibility relationships between cultivars is highly valuable for the appropriate selection of apricot cultivars in commercial orchards and of parental genotypes in breeding programs. The approach described can be transferred to other woody perennial crops with similar problems.

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“…Reports show that pollination compatibility is an important factor affecting fruit set (Austin et al 1996;. Fruit set in the method of controlled pollination under field conditions has a disadvantage, which that the fruit set varies from year to year, depending on weather conditions (Milatović et al, 2010(Milatović et al, , 2013.…”

Section: Controlled Pollination In the Field (Cross Pollination)mentioning

confidence: 99%

Contribution to the study of some aspects of pollination in six varieties of apricot in the region of M'sila (Algeria)

Bendif¹,

Bahlouli²

2017

jsa

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The present work consists in contributing to the study of pollination. Field observations and tests were carried out on six varieties of apricot in the region of M'sila, "Pavit", "Boulida" "Alarbi","Tounsi","Ben sarmouk" and "Louzi rouge". For natural self-pollination, the branches were covered to avoid crosspollination, and the fruit set was determined. Controlled pollination was carried out using pollen and pollen from the other trees that bloom at about the same time. The fruit set rate was determined after counting the fruits in relation to the number of blooming flowers. The rate of fruit set varies from one variety to another. Alarbi with 62.5%, Louzi with 69.7%, Tounssi with 56.5%, Bulida with 50.7%, Ben Sermouk with 23.2% and Pavit with 45.8%. The bagging rate of the bagged branch obtained at the end of the physiological fall did not show any significant differences between the varieties and ranged between 77.50% for Alarbi and 41.22% for Pavit. The results show that the number of fruits after manual crossing is zero for all crops. All varieties tested are self-compatible and no cross-compatibility group has been guessed on the tested growths, from self-pollination and inter-pollination.

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Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

Cited by 13 publications

References 28 publications

Development of an HRMA-Based Marker Assisted Selection (MAS) Approach for Cost-Effective Genotyping of S and M Loci Controlling Self-Compatibility in Apricot (Prunus armeniaca L.)

Development of an HRMA-Based Marker Assisted Selection (MAS) Approach for Cost-Effective Genotyping of S and M Loci Controlling Self-Compatibility in Apricot (Prunus armeniaca L.)

Optimizing Production in the New Generation of Apricot Cultivars: Self-incompatibility, S-RNase Allele Identification, and Incompatibility Group Assignment

Contribution to the study of some aspects of pollination in six varieties of apricot in the region of M'sila (Algeria)

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