Testing the combined effects of probiotics and prebiotics against neurotoxic effects of propionic acid orally administered to rat pups

Suhaibani, Anwar Al; Bacha, Abir Ben; Alonazi, Mona; Bhat, Ramesa Shafi; El‐Ansary, Afaf

doi:10.1002/fsn3.2418

Cited by 11 publications

(7 citation statements)

References 66 publications

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“…Table 1 and Figure 1 demonstrate the significant decrease in GPX1 and GSH observed in both PPA-treated groups in the rodent model of ASD. This result can find support in the recent work carried out by Al Suhaibani et al [ 39 ], in which PPA-treated animals demonstrated a significant reduction in GSH compared with controls. Additional support can be also found in the previous studies by Macfabe et al and El-Ansary et al [ 30 , 40 ], who both reported reductions in GSH in PPA-treated rats.…”

Section: Discussionsupporting

confidence: 85%

Independent and Combined Effects of Probiotics and Prebiotics as Supplements or Food-Rich Diets on a Propionic-Acid-Induced Rodent Model of Autism Spectrum Disorder

et al. 2022

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The link between nutrition and autism spectrum disorder (ASD) as a neurodevelopmental condition, which is clinically presented as significant delays or deviations in interaction and communication, has provided a fresh point of view and signals that nutrition may play a role in the etiology of ASD, as well as playing an effective role in treatment by improving symptoms. In this study, 36 male albino rat pups were used. They were randomly divided into five groups. The control group was fed only a standard diet and water for the 30 days of the experiment. The second group, which served as a propionic acid (PPA)-induced rodent model of ASD, received orally administered PPA (250 mg/kg body weight (BW)) for 3 days, followed by feeding with a standard diet until the end of the experiment. The three other groups were given PPA (250 mg/kg body weight (BW)) for 3 days and then fed a standard diet and orally administered yogurt (3 mL/kg BW/day), artichokes (400 mL/kg BW/day), and a combination of Lacticaseibacillus rhamnosus GG at 0.2 mL daily (1 × 109 CFU; as the probiotic of yogurt) and luteolin (50 mg/kg BW/day; as the major antioxidant and anti-inflammatory ingredient of artichokes) for 27 days. Biochemical markers, including gamma-aminobutyric acid (GABA), reduced glutathione (GSH), glutathione peroxidase (GPx1), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10), were measured in brain homogenates in all groups. The data showed that while PPA demonstrated oxidative stress and neuroinflammation in the treated rats, yogurt, Lacticaseibacillus rhamnosus GG as a probiotic, and luteolin as a prebiotic ingredient in artichokes were effective in alleviating the biochemical features of ASD. In conclusion, nutritional supplementation seems to be a promising intervention strategy for ASD. A combined dietary approach using pro- and prebiotics resulted in significant amelioration of most of the measured variables, suggesting that multiple interventions might be more relevant for the improvement of biochemical autistic features, as well as psychological traits. Prospective controlled trials are needed before recommendations can be made regarding the ideal ASD diet.

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Section: Discussionsupporting

confidence: 85%

Independent and Combined Effects of Probiotics and Prebiotics as Supplements or Food-Rich Diets on a Propionic-Acid-Induced Rodent Model of Autism Spectrum Disorder

et al. 2022

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“…The final aim of the study was to investigate the prebiotic potential of MOS with three probiotic strains: S. thermophilus, B. subtilis, and L. bulgaricus. The cell viability and cell density were tested to determine whether prebiotic MOS can stimulate the growth and metabolic activity of the probiotics under investigation [53]. The probiotic bacteria B. subtilis was investigated for its ability to utilise SBM-produced prebiotic sugars with great success.…”

Section: Discussionmentioning

confidence: 99%

Covalent Immobilisation of an Aspergillus niger Derived Endo-1,4-β-Mannanase, Man26A, on Glutaraldehyde-Activated Chitosan Nanoparticles for the Effective Production of Prebiotic MOS from Soybean Meal

et al. 2022

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An Aspergillus niger endo-1,4-β-mannanase, Man26A, was confirmed by FTIR and XRD to be immobilised on glutaraldehyde-activated chitosan nanoparticles via covalent bonding. The immobilisation (%) and activity yields (%) were 82.25% and 20.75%, respectively. The biochemical properties (pH, temperature optima, and stability) were then comparatively evaluated for both the free and immobilised Man26A. The optimal activity of Man26A shifted to a lower pH after immobilisation (pH 2.0–3.0, from pH 5 for the free enzyme), with the optimum temperature remaining unchanged (60 °C). The two enzymes exhibited identical thermal stability, maintaining 100% activity for the first 6 h at 55 °C. Substrate-specific kinetic analysis showed that the two enzymes had similar affinities towards locust bean gum (LBG) with varied Vmax values. In contrast, they showed various affinities towards soybean meal (SBM) and similar Vmax values. The immobilised enzyme was then employed in the enhancement of the functional feed/prebiotic properties of SBM from poultry feed, increasing mannooligosaccharides (MOS) quantities. The SBM main hydrolysis products were mannobiose (M2) and mannose (M1). The SBM-produced sugars could be utilised as a carbon source by probiotic bacteria; Streptococcus thermophilus, Bacillus subtilis, and Lactobacillus bulgaricus. The results indicate that the immobilised enzyme has the potential for use in the sustainable and cost-effective production of prebiotic MOS from agricultural biomass.

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“…Long-term treatment with Lactobacillus rhamnosus JB-1 influenced the mRNA expressions of GABA receptors in the brain, regulated the gut–brain axis in a vagal-dependent manner, and reduced the depression- and anxiety-like behaviors of pressure-induced mice ( Bravo et al., 2011 ). The treatment of probiotics and prebiotics also effectively restored the level of GABA and normalized the levels of lipid peroxidation and antioxidant glutathione in the brain of rats with cerebral poisoning ( Al Suhaibani et al., 2021 ), indicating that GABA produced by the gut microbiota had great potential in regulating oxidative stress associated with neurodegenerative diseases. Given that gut microbiota has been shown to be influenced by PP and that the level of cerebral GABA was altered significantly, the GABA/Nrf2 pathway might be changed by the communication between PP and the gut–brain axis.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis

Pan

Wei

Yuan

et al. 2022

Front. Cell. Infect. Microbiol.

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Recent evidence demonstrated that functional bacteria were involved in the regulation of Parkinson’s disease (PD). However, the mechanism of probiotics in improving PD was unclear. Here the antioxidant effect and the mechanism of probiotics Pediococcus pentosaceus (PP) on PD were studied by regulating the gut–brain axis. In this study, male C57BL/6J mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneally to establish a PD model and were then treated with PP for 4 weeks. Subsequently, a series of neurobehavioral tests to evaluate the motor function of the mice was performed. Additionally, degeneration of dopaminergic neurons, accumulation of α-synuclein, the production of an oxidative stress response, and the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related proteins were evaluated. Moreover, the gut microbial composition and the level of metabolite γ-aminobutyric acid (GABA) were assessed. The results showed that PP treatment could improve MPTP-induced motor deficits, the degeneration of dopaminergic neurons, and the accumulation of α-synuclein. Moreover, PP treatment significantly increased the levels of SOD1, Gpx1, and Nrf2, while it decreased the levels of Keap1 in the brain of MPTP-induced mice. Notably, PP treatment improved the gut microbial dysbiosis and increased the level of GABA in MPTP-induced mice. These findings indicated that PP might represent a promising candidate, due to the metabolite of GABA, that could be used for the treatment of PD.

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Testing the combined effects of probiotics and prebiotics against neurotoxic effects of propionic acid orally administered to rat pups

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 66 publications

Independent and Combined Effects of Probiotics and Prebiotics as Supplements or Food-Rich Diets on a Propionic-Acid-Induced Rodent Model of Autism Spectrum Disorder

Independent and Combined Effects of Probiotics and Prebiotics as Supplements or Food-Rich Diets on a Propionic-Acid-Induced Rodent Model of Autism Spectrum Disorder

Covalent Immobilisation of an Aspergillus niger Derived Endo-1,4-β-Mannanase, Man26A, on Glutaraldehyde-Activated Chitosan Nanoparticles for the Effective Production of Prebiotic MOS from Soybean Meal

Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis

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