The aim of this study was to evaluate the adequacy of knowledge regarding testicular self-examination (TSE) in final-year medical students and determine the TSE performance rate among male students. Materials and Methods: This cross-sectional study was conducted with final-year students in the Meram Medical Faculty of Necmettin Erbakan University in the 2015-2016 academic year. The target population of the study was 233 people and all 202 people who agreed to participate in the survey were included. A data collection form consisting of 29 questions was prepared for the study and was completed under observation. Questions regarding TSE technique and knowledge of TSE and testicular cancer were scored as 1 point for each correct answer and 0 points for incorrect answers. Scores ≥6 points for TSE technique and ≥10 points for TSE and testicular cancer knowledge were regarded as adequate. Results: The mean age of the participants was 24.12±1.32 years; 44.1% were women and 89.6% were single. Nearly 25% of the students reported they knew how to perform TSE and 32.1% had performed TSE before. History of cancer in a first-degree relative was reported by 17.4% of the students, but no students had family history of testicular cancer. Fifty-three of the students who claimed to know how to perform TSE, only 34% (n=18) scored at least 6 points in the TSE technique questions. Evaluation of scores in the TSE and testicular cancer knowledge section showed that 21.3% (n=37) scored above the 10 point limit. Conclusion: In our study, it was observed that most of the final-year medical students did not have sufficient information on TSE and testicular cancer, and that TSE rates of male students were low.